1887

Abstract

SUMMARY

Neutralizing monoclonal antibodies raised against type O foot-and-mouth disease virus have been characterized on the basis of their reactivity with a panel of single site monoclonal antibody-resistant mutants which had defined three antigenic sites. Five antibodies neutralized all these mutants, but by selecting further single site mutants with one of these antibodies it was possible to define a fourth site involved in virus neutralization. Two monoclonal antibodies still neutralized these mutants and all multiple site resistant mutants. One multiple site resistant mutant was resistant to neutralization at each of four antigenic sites but was still efficiently neutralized by type O convalescent cattle sera. The relationship between sites recognized by different monoclonal antibodies generated in different laboratories is discussed.

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/content/journal/jgv/10.1099/0022-1317-70-3-639
1989-03-01
2022-12-01
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References

  1. Blondel B., Crainic R., Fichot O., Dufraisse G., Candrea A., Diamond D., Girard M., Horaud F. 1986; Mutations conferring resistance to neutralization with monoclonal antibodies in type 1 poliovirus can be located outside or inside the antibody binding site. Journal of Virology 57:81–90
    [Google Scholar]
  2. Crowther I. R., McCullough K. C., De Simone F., Brocchi E. 1984; Monoclonal antibodies against FMDV: application and potential use. Report to the Research Group of the Standing Technical Committee of the European Commission for the Control of FMD (Brescia, Italy)40–51 Rome: F.A.O;
    [Google Scholar]
  3. Duchesne M., Cartwright T., Crespo A., Boucher F., Fallourd A. 1984; Location of a neutralization epitope of foot-and-mouth disease virus using neutralizing monoclonal antibodies. Journal of General Virology 65:1559–1566
    [Google Scholar]
  4. Fox G., Stuart D., Acharya K. R., Fry F., Rowlands D., Brown F. 1987; Crystallization and preliminary X- ray diffraction analysis of FMDV. Journal of Molecular Biology 196:591–597
    [Google Scholar]
  5. Hogle J. M., Chow M., Filman D. J. 1985; The three-dimensional structure of poliovirus at 2·9 Å resolution. Science 229:1358–1365
    [Google Scholar]
  6. Mccullough K. C., Crowther I. R., Butcher R. N. 1985; Alteration in antibody reactivity with foot-and-mouth disease virus 146S antigen before and after binding to a solid phase of complexing with specific antibody. Journal of Immunological Methods 82:91–100
    [Google Scholar]
  7. Mccullough K. C., Crowther J. R., Carpenter W. G., Brocchi E., Capucci L., De Simone F., Xie Q., Mccahon D. 1987; Epitopes on foot-and-mouth disease virus particles. Virology 157:516–525
    [Google Scholar]
  8. Meloen R. H., Briaire J., Woortmeyer R. J., Van Zaane D. 1983; The main antigenic determinant detected by neutralizing monoclonal antibodies on the intact foot-and-mouth disease virus particle is absent from isolated VP1. Journal of General Virology 64:1193–1198
    [Google Scholar]
  9. Minor P. D., Ferguson M., Evans D. M. A., Almond J. W., Icenogle J. P. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67:1283–1291
    [Google Scholar]
  10. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffiths J. P., Hecht H. Z., Johnson J. E., Kramor G., Luo M., Mosser A. G., Rueckert R. R., Sherry B., Vriend G. 1985; The structure of a human common cold virus (rhinovirus 14) and its functional relations to other picornaviruses. Nature, London 317:145–153
    [Google Scholar]
  11. Rowlands D. J., Clarke B. E., Carroll A. R., Brown F., Nicholson B. H., Bittle J. L., Houghten R. A., Lerner R. A. 1983; Chemical basis of antigenic variation in foot-and-mouth disease virus. Nature, London 306:694–697
    [Google Scholar]
  12. Sherry B., Mosser A. G., Colonno R. I., Rueckert R. R. 1986; Use of monoclonal antibodies to identify four neutralization immunogens on a common cold picornavirus, human rhinovirus 14. Journal of Virology 57:246–257
    [Google Scholar]
  13. Stave J. W., Cand J. L., Morgan D. O., Vakharia V. N. 1988; Neutralization sites of type Ol foot-and-mouth disease virus defined by monoclonal antibodies and neutralization-escape variants. Virology 162:21–29
    [Google Scholar]
  14. Xie Q-C., Mccahon D., Crowther J. R., Belsham G. J., Mccullough K. C. 1987; Neutralization of foot-and-mouth disease virus can be mediated through any of at least three separate antigenic sites. Journal of General Virology 68:1637–1647
    [Google Scholar]
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