1887

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Volume 70, Issue 3

Differential Expression of Interferon Alpha and Beta Induced with Newcastle Disease Virus in Mouse Macrophage Cultures Free

Abstract

SUMMARY

Stimulation of mouse macrophages with Newcastle disease virus (NDV) leads to a rapid and high interferon (IFN) response. The magnitude of this response is influenced by the mouse genotype. We have analysed NDV-induced IFN production at the protein and mRNA levels in two different populations of macrophages derived from ‘high producer’ C57BL/6 and ‘low producer’ BALB/c mice . The data indicate that bone marrow and peritoneal macrophages from both strains grown in the presence of L cell conditioned medium (CM) as a source of macrophage colony-stimulating factor 1 (M-CSF) or purified murine M-CSF produce 10- to 50-fold more IFN on a per cell basis than cultures of resident peritoneal macrophages. These differences were also found when steady state levels of IFN mRNA were analysed. Differential analysis for the ratios of IFN- and IFN- showed that CM- or M-CSF-cultured macrophages produced equal amounts of both IFN species as determined by specific monoclonal antibodies and hybridization experiments using IFN- and IFN- DNA probes, whereas resident peritoneal macrophages induced under identical conditions produced almost exclusively IFN-. This suggests a stimulating effect of M-CSF on IFN synthesis in NDV-induced cultures of mouse macrophages, which is in part due to additional activation of IFN- gene expression.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): -β) , hybridization , interferon (MuIFN-α and NDV
©Society for General Microbiology 1989
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1989-03-01
2024-04-19
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Differential Expression of Interferon Alpha and Beta Induced with Newcastle Disease Virus in Mouse Macrophage Cultures
J. Gen. Virol. 70, 575 (1989); https://doi.org/10.1099/0022-1317-70-3-575
/content/journal/jgv/10.1099/0022-1317-70-3-575
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