1887

Abstract

Summary

Inflammatory cells were isolated from West Nile virus (WNV)-infected CBA/H (H-2) mouse brains, and their function and cell surface markers were studied. Two populations of cytolytic lymphocytes were detected. One population, which lysed WNV-infected and uninfected L929 (H-2), YAC-1 (H-2), P815 (H-2), OH (H-2KD) and 2R (H-2KD) target cells, had a phenotype of L3T4 Lyt2 Thyl asialo GM1 and hence were natural killer (NK) cells. The other, which lysed WNV-infected cells to a greater extent than uninfected L929 cells, had a phenotype of L3T4 Lyt2 Thy1 asialo GM1 and were cytotoxic T cells. In addition, the presence of the latter population was demonstrated by the specific lysis of syngeneic WNV-infected astrocytes, a cell type which is resistant to NK cell lysis. The cell surface markers of isolated inflammatory cells were determined by two colour flow cytometry. This showed that 15 to 40% of the cells were Thyl, among which about 3% were Lyt2. No L3T4 cells were detected.

Keyword(s): CNS , cytolytic lymphocytes and WNV
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/content/journal/jgv/10.1099/0022-1317-70-3-565
1989-03-01
2019-11-12
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-3-565
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