Inflammatory cells were isolated from West Nile virus (WNV)-infected CBA/H (H-2) mouse brains, and their function and cell surface markers were studied. Two populations of cytolytic lymphocytes were detected. One population, which lysed WNV-infected and uninfected L929 (H-2), YAC-1 (H-2), P815 (H-2), OH (H-2KD) and 2R (H-2KD) target cells, had a phenotype of L3T4 Lyt2 Thyl asialo GM1 and hence were natural killer (NK) cells. The other, which lysed WNV-infected cells to a greater extent than uninfected L929 cells, had a phenotype of L3T4 Lyt2 Thy1 asialo GM1 and were cytotoxic T cells. In addition, the presence of the latter population was demonstrated by the specific lysis of syngeneic WNV-infected astrocytes, a cell type which is resistant to NK cell lysis. The cell surface markers of isolated inflammatory cells were determined by two colour flow cytometry. This showed that 15 to 40% of the cells were Thyl, among which about 3% were Lyt2. No L3T4 cells were detected.

Keyword(s): CNS , cytolytic lymphocytes and WNV

Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error