1887

Abstract

SUMMARY

Inflammatory cells were isolated from West Nile virus (WNV)-infected CBA/H (H-2) mouse brains, and their function and cell surface markers were studied. Two populations of cytolytic lymphocytes were detected. One population, which lysed WNV-infected and uninfected L929 (H-2), YAC-1 (H-2), P815 (H-2), OH (H-2KD) and 2R (H-2KD) target cells, had a phenotype of L3T4 Lyt2 Thy1 asialo GM1 and hence were natural killer (NK) cells. The other, which lysed WNV-infected cells to a greater extent than uninfected L929 cells, had a phenotype of L3T4 Lyt2 Thy1 asialo GM1 and were cytotoxic T cells. In addition, the presence of the latter population was demonstrated by the specific lysis of syngeneic WNV-infected astrocytes, a cell type which is resistant to NK cell lysis. The cell surface markers of isolated inflammatory cells were determined by two colour flow cytometry. This showed that 15 to 40% of the cells were Thy1, among which about 3% were Lyt2. No L3T4 cells were detected.

Keyword(s): CNS , cytolytic lymphocytes and WNV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-3-565
1989-03-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/3/JV0700030565.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-3-565&mimeType=html&fmt=ahah

References

  1. Allan J. E., Doherty P. C. 1986; Natural killer cells contribute to inflammation but do not appear to be essential for the induction of clinical lymphocytic choriomeningitis. Scandinavian Journal of Immunology 24:153–162
    [Google Scholar]
  2. Allan J. E., Dixon J. E., Doherty P. C. 1987; Nature of the inflammatory process in the central nervous system of mice infected with lymphocytic choriomeningitis virus. Current Topics in Microbiology and Immunology 134:131–143
    [Google Scholar]
  3. Baenziger J., Hengartner H., Zinkernagel R. M., Cole G. A. 1986; Induction or prevention of immunopathological disease by cloned cytotoxic T cell lines specific for lymphocytic choriomeningitis virus. European Journal of Immunology 16:387–393
    [Google Scholar]
  4. Berger M. L. 1980; Humoral and cell mediated immune mechanisms in the production of pathology in avirulent Semliki Forest virus encephalitis. Infection and Immunity 30:244–253
    [Google Scholar]
  5. Brier A. M., Synderman R., Mergenhagen S. E., Notkins A. L. 1970; Inflammation and herpes simplex virus release of a chemotaxis generating factor from infected cells. Science 170:1104–1106
    [Google Scholar]
  6. Cantor H., Boyse E. A. 1975; Functional subclasses of T lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T cell subclasses is a differentiative process independent of antigen. Journal of Experimental Medicine 141:1376–1389
    [Google Scholar]
  7. Ceredig R., Lowenthal J. W., Nabholz M., Mcdonald H. R. 1985; Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells. Nature, London 314:98–100
    [Google Scholar]
  8. Ceredig R., Allan J. E., Tabi Z., Lynch F., Doherty P. C. 1987; Phenotypic analysis of the inflammatory exudate in murine lymphocytic choriomeningitis. Journal of Experimental Medicine 165:1539–1551
    [Google Scholar]
  9. Cole G. A. 1968; Potentiation of experimental arbovirus encephalitis by immunosuppressive doses of cyclophosphamide. Nature, London 220:399–401
    [Google Scholar]
  10. Dialynas D. P., Quan Z. S., Wall K. A., Pierres A., Quintans J., Loken M. R., Pierres M., Fitch F. W. 1983a; Characterization of the murine T cell surface molecule, designated L3T4 identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. Journal of Immunology 131:2445–2451
    [Google Scholar]
  11. Dialynas D. P., Wilde D. B., Marrack P., Pierres A., Wall K. A., Havran W., Otten G., Loken M. R., Pierres M., Kappler J., Fitch F. W. 1983b; Characterization of the murine antigenic determinant designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen reactivity. Immunological Reviews 74:29–56
    [Google Scholar]
  12. Doherty P. C. 1973; Quantitative studies of inflammatory process in fatal viral meningoencephalitis. American Journal of Pathology 73:607–622
    [Google Scholar]
  13. Doherty P. C., Korngold W. 1983; Characteristics of viral meningoencephalitis: distribution of natural killer cells and cytotoxic T lymphocytes in a poxvirus induced inflammatory exudate. Scandinavian Journal of Immunology 15:1–7
    [Google Scholar]
  14. Doherty P. C., Zinkernagel R. M. 1974; T cell mediated immunopathology in viral infection. Transplantation Reviews 19:89–120
    [Google Scholar]
  15. Erard F., Nabholz M., Dupuy-D’Angeac A., Mcdonald H. R. 1985; Differential requirements for the induction of interleukin 2 responsiveness in L3T4+ and Lyt2+ T cells subsets. Journal of Experimental Medicine 162:1738–1743
    [Google Scholar]
  16. Gardner I. D., Bowern N. A., Blanden R. V. 1975; Cell mediated cytotoxicity against ectromelia virus-infected target cells. European Journal of Immunology 5:122–127
    [Google Scholar]
  17. Gates M. C, Sheahan B. J., Atkins G. J. 1984; The pathogenicity of the M9 mutant of Semliki Forest virus in immune-compromised mice. Journal of General Virology 65:73–80
    [Google Scholar]
  18. Griffin D. E., Hess J. L. 1986; Cell with natural killer activity in the cerebrospinal fluid of normal mice and athymic nude mice with acute Sindbis virus encephalitis. Journal of Immunology 136:1841–1845
    [Google Scholar]
  19. Hirsch M. S., Murphy F. A. 1967; Effect of anti-thymocyte serum on 17-D yellow fever infection in adult mice. Nature, London 216:179–180
    [Google Scholar]
  20. Hirsch R. L. 1981; Natural killer cells appear to play no role in the recovery of mice from Sindbis virus infection. Immunology 43:81–89
    [Google Scholar]
  21. Jacoby R. O., Bhatt P. N., Schwartz A. 1980; Protection of mice from lethal flavivirus encephalitis by adoptive transfer of splenic cells from donors infected with live virus. Journal of Infectious Diseases 141:617–624
    [Google Scholar]
  22. Liu Y., Müllbacher A. 1988; Astrocytes are not susceptible to natural killer cell lysis. Journal of Neuroimmunology 19:101–110
    [Google Scholar]
  23. Liu Y., King N., Kesson A., Blanden R. V., Mullbacher A. 1988; West Nile virus infection up-regulates the expression of class I and class II MHC antigens on and enhances T cell recognition of astrocytes in vitro . Journal of Neuroimmunology (in press)
    [Google Scholar]
  24. McCarthy K. D., De Vellis J. 1980; Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. Journal of Cell Biology 85:890–902
    [Google Scholar]
  25. Marshak-Rothstein A., ., Fink P., Gridley T., Raulet D. H., Bevan M. J., Gefter M. L. 1979; Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. Journal of Immunology 122:2491–2497
    [Google Scholar]
  26. Mathur A., Arora K. L., Chaturvedi U. C. 1983; Host defence mechanisms against Japanese encephalitis virus infection in mice. Journal of General Virology 64:805–811
    [Google Scholar]
  27. Moench T. R., Griffin D. E. 1984; Immunocytochemical identification and quantitation of mononuclear cells in cerebrospinal fluid, meninges, and brain during acute viral encephalitis. Journal of Experimental Medicine 159:77–88
    [Google Scholar]
  28. Monath T. P. 1986; Pathobiology of flaviviruses. In The Togaviridae and Flaviviridae375–440 Schlesinger S., Schlesinger M. J. New York & London: Plenum Press;
    [Google Scholar]
  29. Müllbacher A., Ashman R. B., Ada G. L. 1984; Alloreactive cytotoxic lymphocytes lyse syngeneic influenza-infected tumor cell targets. Scandinavian Journal of Immunology 19:365–371
    [Google Scholar]
  30. Oldstone M. B. A., Blount P., Southern P. J., Lampert P. W. 1986; Cytoimmunotherapy for persistent virus infection reveals a unique clearance pattern for the central nervous system. Nature, London 321:239–243
    [Google Scholar]
  31. Sarmiento M., Glasebrook A. L., Fitch F. W. 1980; IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt2 antigen block T cell-mediated cytolysis in the absence of complement. Journal of Immunology 125:2665–2672
    [Google Scholar]
  32. Storkus W. J., Howell D. N., Salter R. D., Dawson J. R., Cresswell P. 1987; NK susceptibility varies inversely with target cell class I HLA antigen expression. Journal of Immunology 138:1657–1659
    [Google Scholar]
  33. Taylor W. P., Marshall I. D. 1975; Adaptation studies with Ross River virus: laboratory mice and cell cultures. Journal of General Virology 28:59–72
    [Google Scholar]
  34. Webb H. E., Wight D. G. D., Wiernik G., Platt G. S., Smith C. E. G. 1968; Langat virus encephalitis in mice. II. The effect of irradiation. Journal of Hygiene 66:355–364
    [Google Scholar]
  35. Williams A. F., Gagnon J. 1982; Neuronal cell surface Thy-1 glycoprotein: homology and immunoglobulin. Science 216:696–703
    [Google Scholar]
  36. Wong G. H. W., Bartlett P. F., Clark-Lewis I., ., Mckimm-Breschkin I. L., Schrader I. W. 1985; Interferon-γ induces the expression of H-2 and 1a antigen on brain cells. Journal of Neuroimmunology 7:255–278
    [Google Scholar]
  37. Yap K. L., Ada G. L. 1977; Cytotoxic T cells specific for influenza virus infected target cells. Immunology 32:151–159
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-3-565
Loading
/content/journal/jgv/10.1099/0022-1317-70-3-565
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error