1887

Abstract

Summary

Three spontaneous mutants of strain TpM-34 of red clover necrotic mosaic virus, designated M-A, M-B and M-C, were isolated. In gel diffusion tests the three mutants were serologically indistinguishable from the parent but when inoculated onto each induced characteristic symptoms which differed from those induced by TpM-34. TpM-34 induced similar numbers of lesions at 17 °C and 26 °C. However, although inoculation with RNA of the mutants induced 70 to 80% as many lesions as inoculation with TpM-34 RNA at 17 °C, very few lesions were induced by any of the mutants at 26 °C. The symptoms induced by heterologous mixtures of RNA 1 or RNA 2 of TpM-34 with RNA 2 or RNA 1 of each mutant indicated that M-A, M-B and M-C each has mutations in both RNA components, but that symptom expression was determined predominantly by RNA 1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-2-491
1989-02-01
2019-10-15
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-2-491
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error