Seven monoclonal antibodies (MAbs) were produced which recognized equid herpesvirus type 1 (EHV-1). Three MAbs neutralized the subtype 1 virus (strain Army 183) in the presence of complement, but did not neutralize the subtype 2 virus (strain MD). All three MAbs immunoprecipitated an Mr 83K glycoprotein from a detergent-solubilized virion envelope preparation of the subtype 1 virus. The target antigens of the four non-neutralizing MAbs (6F11, 2A4, 1F10 and 8D9) were identified by immunoblotting against purified EHV-1 virions and had respective apparent Mr values of > 205K, > 205K, 97K and 13K.
AllenG. P.,
bryansJ. T.1986; Molecular epizootiology, pathogenesis and prophylaxis of equine herpesvirus-1 infections.. Progress in Veterinary Microbiology and Immunology 2:78–144
AllenG. P.,
YearganM. R.1987; Use of λgt11 and monoclonal antibodies to map the genes for the six major glycoproteins of equine herpesvirus-1.. Journalof Virology 61:2454–2461
BurrowsR. D.,
GoodridgeD.1972; In vivo and in vitro studies of equine rhinopneumonitis strains. In Proceedings of the Third International Conference on Equine Infectious Diseases306–321BryansJ. T.,
GerberH.
Basel: S. Karger;
Papp-ViddG.,
DerbyshireJ. B.1979; The virus neutralizing activity of antibodies specific to the envelope and nucleocapsid of equine herpesvirus type-1.. Canadian Journalof Comparative Medicine 43:231–233
SabineM.,
RobertsonG. R.,
WhalleyJ. M.1981; Differentiation of subtypes of equine herpesvirus 1 by restriction endonuclease analyses.. Australian Veterinary Journal 57:148–149
StuddertM. J.,
SimpsonT.,
RoizmanB.1981; Differentiation of respiratory and abortogenic isolates of equine herpesvirus 1 by restriction endonucleases.. Science 214:562–564
ThomsonG. R.,
MumfordJ. A.,
CampbellJ.,
GriffithL.,
ClaphamP.1976; Serological detection of equid herpesvirus 1 infections in the respiratory tract.. Equine Veterinary Journal 8:58–65
TurtinenL. W.,
AllenG. P.1982; Identification of the envelope surface glycoproteins of equine herpesvirus type 1.. Journalof General Virology 63:481–485
TurtinenL. W.,
AllenG. P.,
DarlingtonR. W.,
BryansJ. T.1981; Serological and molecular comparisons of several equine herpesvirus type 1 strains.. American Journalof Veterinary Research 42:2099–2104