The Characterization of Neutralizing and Non-neutralizing Monoclonal Antibodies against Equid Herpesvirus Type 1 Free

Abstract

SUMMARY

Seven monoclonal antibodies (MAbs) were produced which recognized equid herpesvirus type 1 (EHV-1). Three MAbs neutralized the subtype 1 virus (strain Army 183) in the presence of complement, but did not neutralize the subtype 2 virus (strain MD). All three MAbs immunoprecipitated an 83K glycoprotein from a detergent-solubilized virion envelope preparation of the subtype 1 virus. The target antigens of the four non-neutralizing MAbs (6F11, 2A4, 1F10 and 8D9) were identified by immunoblotting against purified EHV-1 virions and had respective apparent values of > 205K, > 205K, 97K and 13K.

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1989-02-01
2024-03-28
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References

  1. Allen G. P., bryans J. T. 1986; Molecular epizootiology, pathogenesis and prophylaxis of equine herpesvirus-1 infections.. Progress in Veterinary Microbiology and Immunology 2:78–144
    [Google Scholar]
  2. Allen G. P., Yeargan M. R. 1987; Use of λgt11 and monoclonal antibodies to map the genes for the six major glycoproteins of equine herpesvirus-1.. Journalof Virology 61:2454–2461
    [Google Scholar]
  3. Bryans J. T. 1980; Herpesviral disease affecting reproduction in the horse.. Veterinary Clinics of North America, Large Animal Practice 2:303–312
    [Google Scholar]
  4. Burrows R. D., Goodridge D. 1972; In vivo and in vitro studies of equine rhinopneumonitis strains. In Proceedings of the Third International Conference on Equine Infectious Diseases306–321 Bryans J. T., Gerber H. Basel: S. Karger;
    [Google Scholar]
  5. Campbell A. M. 1984 In Laboratory Techniques in Biochemistry and Molecular Biology 13 Amsterdam: Elsevier;
    [Google Scholar]
  6. Cook R. F., Avery R. J., Dimmock N. J. 1979; Infection of chicken erythrocytes with influenza and other viruses.. Infection and Immunity 25:396–402
    [Google Scholar]
  7. Cook R. F., Sinclair R., Mumford J. A. 1988; Detection of influenza nucleoprotein antigen in nasal secretions from horses infected with A/equine/influenza (H3N8) viruses.. Journalof Virological Methods 20:1–12
    [Google Scholar]
  8. Edington N., Bridges C. G., Broad S. C. 1987; Rapid diagnosis and characterization of equid herpesvirus 1 using monoclonal antibodies.. Tierarztliche Praxis Supplement 2:37–40
    [Google Scholar]
  9. Hall M. J., Katrak K. 1986; The quest for a herpes simplex virus vaccine: background and recent developments.. Vaccine 4:138–150
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 227:680–685
    [Google Scholar]
  11. Papp-Vidd G., Derbyshire J. B. 1979; The virus neutralizing activity of antibodies specific to the envelope and nucleocapsid of equine herpesvirus type-1.. Canadian Journalof Comparative Medicine 43:231–233
    [Google Scholar]
  12. Sabine M., Robertson G. R., Whalley J. M. 1981; Differentiation of subtypes of equine herpesvirus 1 by restriction endonuclease analyses.. Australian Veterinary Journal 57:148–149
    [Google Scholar]
  13. Studdert M. J., Simpson T., Roizman B. 1981; Differentiation of respiratory and abortogenic isolates of equine herpesvirus 1 by restriction endonucleases.. Science 214:562–564
    [Google Scholar]
  14. Thomson G. R., Mumford J. A., Campbell J., Griffith L., Clapham P. 1976; Serological detection of equid herpesvirus 1 infections in the respiratory tract.. Equine Veterinary Journal 8:58–65
    [Google Scholar]
  15. Turtinen L. W. 1983 Studies on the antigenic and genetic variation between the two subtypes of equine herpesvirus-1. Ph.D. thesis University of Kentucky;
    [Google Scholar]
  16. Turtinen L. W., Allen G. P. 1982; Identification of the envelope surface glycoproteins of equine herpesvirus type 1.. Journalof General Virology 63:481–485
    [Google Scholar]
  17. Turtinen L. W., Allen G. P., Darlington R. W., Bryans J. T. 1981; Serological and molecular comparisons of several equine herpesvirus type 1 strains.. American Journalof Veterinary Research 42:2099–2104
    [Google Scholar]
  18. Yeargan M. R., Allen G. P., Bryans J. T. 1985; Rapid subtyping of equine herpesvirus-1 isolates with monoclonal antibodies.. Journalof Clinical Microbiology 21:644–697
    [Google Scholar]
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