1887

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Volume 70, Issue 2

Interferon Induction by Viruses. XVII. Non-temperature-sensitive Mutations Regulate Interferon Induction by Vesicular Stomatitis Virus Free

Abstract

SUMMARY

Wild-type (wt) strains of vesicular stomatitis virus (VSV) strain Indiana are poor to non-inducers of interferon (IFN) which express IFN induction-suppressing activity. At non-permissive temperatures, temperature-sensitive () mutants of this virus are either like their wt parents, or they are good to excellent inducers of IFN. IFN inducibility and IFN induction-suppressing activity are mutually exclusive phenotypes in VSV-Indiana. With one exception, all Orsay mutants derived by A. Flamand (CNRS, Gif-sur-Yvette, France), representing the five complementation groups, were poor to non-inducers of IFN and were also capable of suppressing IFN induction by other viruses. In contrast, all Glasgow mutants derived by C. R. Pringle (University of Warwick, Coventry, U.K.) were excellent inducers of IFN. We demonstrate that this difference in acquisition of IFN inducibility relates primarily to the origin of the mutations; spontaneous for Orsay, and mutagen-derived for Glasgow. Tests with newly generated spontaneous and mutagen-derived mutants, and temperature-stable revertants of IFN-inducing mutants indicate that IFN inducibility results from non-, multiple mutations rarely acquired spontaneously, but generated frequently upon mutagenesis with 5-fluorouracil. The capacity of VSV-Indiana to induce IFN is considered intrinsic to the virus, but is only manifested when the dominant IFN induction-suppressing phenotype is lost through mutagenesis. Thus, non- mutations appear to regulate the expression of the IFN induction-suppressing phenotype, and hence the IFN inducibility of VSV-Indiana.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): IFN , temperature-sensitive mutants and VSV
© Society for General Microbiology 1989
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1989-02-01
2024-04-19
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References

  1. Banerjee A. 1987; Transcription And replication of rhabdoviruses. Microbiological Reviews 51:66–87
     [Google Scholar]
  2. Flamand A. 1970; Etude génétique du virus de la stomatite vésiculaire: classement de mutants thermosensibles spontanés en groupes de complémentation. Journal of General Virology 8:187–195
     [Google Scholar]
  3. Flamand A., Pringle C. R. 1971; The homologies of spontaneous and induced temperature-sensitive mutants of vesicular stomatitis virus isolated in chick embryo and BHK 21 cells. Journal of General Virology 11:81–85
     [Google Scholar]
  4. Francoeur A. M., Lam T., Stanners C. P. 1980; PIF, a highly sensitive plaque assay for the induction of interferon. Virology 105:526–536
     [Google Scholar]
  5. Francoeur A. M., Poliquin L., Stanners C. P. 1987; The isolation of interferon-inducing mutants of vesicular stomatitis virus with altered viral P function for the inhibition of total protein synthesis. Virology 160:236–245
     [Google Scholar]
  6. Gallione C. J., Rose J. K. 1985; A single amino-acid substitution in a hydrophobic domain causes temperature-sensitive cell-surface transport of a mutant viral glycoprotein. Journal of Virology 54:374–382
     [Google Scholar]
  7. Grinnell B. W., Wagner R. R. 1984; Nucleotide sequence and secondary structure of VSV leader RNA and homologous DNA involved in inhibition of DNA-dependent transcription. Cell 36:533–543
     [Google Scholar]
  8. Mcgowan J. J., Emerson S. U., Wagner R. R. 1982; The plus-strand leader RNA of vesicular stomatitis virus inhibits DNA-dependent transcription of adenovirus and SV40 genes in a soluble whole-cell extract. Cell 28:325–333
     [Google Scholar]
  9. Marcus P. I. 1982; Interferon induction by viruses. IX. Antagonistic activities of virus particles modulate interferon production. Journal of Interferon Research 2:511–518
     [Google Scholar]
  10. Marcus P. I. 1983; Interferon induction by viruses: one molecule of double-stranded RNA as the threshold for interferon induction. In Interferon 5115–180 Gresser I. New York & London: Academic Press;
     [Google Scholar]
  11. Marcus P. I. 1986; Interferon induction dose-response curves. Methods in Enzymology 119:106–114
     [Google Scholar]
  12. Marcus P. I., Carver D. H. 1965; Hemadsorption-negative plaque test: new assay for rubella virus revealing a unique interference. Science 149:983–986
     [Google Scholar]
  13. Marcus P. I., Sekellick M. J. 1976; Cell killing by viruses. III. The interferon system and inhibition of cell killing by vesicular stomatitis virus. Virology 69:378–393
     [Google Scholar]
  14. Marcus P. I., Sekellick M. J. 1977; Defective interfering particles with covalently linked [ + / – ] RNA induce interferon. Nature, London 226:815–818
     [Google Scholar]
  15. Marcus P. I., Sekellick M. J. 1980; Interferon induction by viruses. III. Vesicular stomatitis virus: interferon-inducing particle activity requires partial transcription of gene N. Journal of General Virology 47:89–96
     [Google Scholar]
  16. Marcus P. I., Sekellick M. J. 1985; Interferon induction by viruses. XIII. Detection and assay of interferon induction-suppressing particles. Virology 142:411–415
     [Google Scholar]
  17. Marcus P. I., Sekellick M. I. 1987; Interferon induction by viruses. XV. Biological characteristics of interferon induction-suppressing particles of vesicular stomatitis virus. Journal of Interferon Research 7:269–284
     [Google Scholar]
  18. Marcus P. I., Svitlik C., Sekellick M. J. 1983; Interferon induction by viruses. X. A model for interferon induction by Newcastle disease virus. Journal of General Virology 64:2419–2431
     [Google Scholar]
  19. Marcus P. I., Gaccione C., Sekellick M. J. 1987; Interferon induction by viruses: a wildtype strain of vesicular stomatitis virus that induces interferon. Journal of Interferon Research 7:714
     [Google Scholar]
  20. Morita K., Vanderoef R., Lenard J. 1987; Phenotypic revertants of temperature-sensitive m protein mutants of vesicular stomatitis virus: sequence analysis and functional characterization. Journal of Virology 61:256–263
     [Google Scholar]
  21. Pringle C. R. 1970; Genetic characteristics of conditional lethal mutants of vesicular stomatitis virus induced by 5-fluorouracil, 5-azacytidine and ethylmethane sulfonate. Journal of Virology 5:559–567
     [Google Scholar]
  22. Pringle C. R. 1975; Conditional lethal mutants of vesicular stomatitis virus. Current Topics in Microbiology and Immunobiology 69:85–116
     [Google Scholar]
  23. Rae B. P., Elliott R. M. 1986; Characterization of the mutations responsible for the electrophoretic mobility differences in the NS proteins of vesicular stomatitis virus New Jersey complementation group E mutants. Journal of General Virology 67:2635–2643
     [Google Scholar]
  24. Rice A. P., Duncan R., Hershey J. W. B., Kerr I. M. 1985; Double-stranded RNA-dependent protein kinase and 2-5 A system are both activated in interferon-treated, encephalomyocarditis virus-infected HeLa cells. Journal of Virology 54:894–898
     [Google Scholar]
  25. Sekellick M. J., Marcus P. I. 1979; Persistent infection. II. Interferon-inducing temperature-sensitive mutants as mediators of cell sparing: possible role in persistent infection by vesicular stomatitis virus. Virology 95:36–47
     [Google Scholar]
  26. Sekellick M. I., Marcus P. I. 1980; Viral interference by defective particles of vesicular stomatitis virus measured in individual cells. Virology 104:247–252
     [Google Scholar]
  27. Sekellick M. J., Marcus P. I. 1985; Interferon induction by viruses. XIV. Development of interferon inducibility and its inhibition in chick embryo cells aged in vitro. Journal of Interferon Research 5:651–667
     [Google Scholar]
  28. Sekellick M. J., Marcus P. I. 1986; Induction of high titer chicken interferon. Methods in Enzymology 119:115–125
     [Google Scholar]
  29. Stanners C. P., Francoeur A. M., Lam T. 1977; Analysis of a vesicular stomatitis virus mutant with attenuated cytopathogenicity: mutation in viral function, P, for inhibition of protein synthesis. Cell 11:273–281
     [Google Scholar]
  30. Wagner R. R., Huang A. S. 1966; Inhibition of RNA and interferon synthesis in Krebs-2-cells infected with vesicular stomatitis virus. Virology 28:1–10
     [Google Scholar]
  31. Wagner R. R., Levy A. H., Snyder R. M., Ratcliff G. A., Hyatt D. F. 1963; Biological properties of two plaque variants of vesicular stomatitis virus. Journal of Immunology 91:112–121
     [Google Scholar]
  32. Wertz G. W., Youngner J. S. 1970; Interferon production and inhibition of host cell synthesis in cells infected with vesicular stomatitis virus. Journal of Virology 6:476–484
     [Google Scholar]
  33. Youngner J. S., Dubovi E. J., Quagliana D. O., Kelly M., Preble O. T. 1976; Role of temperature-sensitive mutants in persistent infections initiated with vesicular somatitis virus. Journal of Virology 19:90–101
     [Google Scholar]
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Interferon Induction by Viruses. XVII. Non-temperature-sensitive Mutations Regulate Interferon Induction by Vesicular Stomatitis Virus
J. Gen. Virol. 70, 405 (1989); https://doi.org/10.1099/0022-1317-70-2-405
/content/journal/jgv/10.1099/0022-1317-70-2-405
/content/journal/jgv/10.1099/0022-1317-70-2-405
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