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Journal of General Virology header logo Journal of General Virology

Volume 70, Issue 2

T Cell-dependent Induction of Antibody against Foot-and-Mouth Disease Virus in a Mouse Model No Access

Abstract

SUMMARY

Nude and normal BALB/c mice were primed by intravenous inoculation of purified, infectious foot-and-mouth disease virus (FMDV) type A24, strain Cruzeiro. Frequency estimation of antigen-specific antibody-secreting cells (ASC) and Thy 1 T cells in the spleens of immunized mice identified that the IgM response was similar for both nude and normal mice, whereas substantial numbers of both IgG ASC and Thy 1 cells were present in normal mice only. In contrast, nude and normal mouse sera both contained IgG although the nude mouse serum was deficient in IgG1. Antigen-specific antibody could not be induced in spleen cell cultures from C57BL/6 mice after depletion of T cells with monoclonal antibody plus complement. However, the antibody response could be reconstituted if either a source of exogenous lymphokines or T cells from primed but not unprimed mice were added. Similarly, polyclonal stimulation or unprimed T cells could restore the response and thus complemented the finding of a low frequency of helper T cells in unprimed mice. Taken together, these data identify that the induction of IgG in FMDV-immunized mice is T cell-dependent and regulated by lymphokines. Furthermore, in nude mice a site other than the spleen must be responsible for the synthesis of the observed serum IgG.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antibody-secreting cells , FMDV and T cell
© Society for General Microbiology 1989
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1989-02-01
2025-03-23
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References

  1. Ballieux R. E., Heijnen C. J., Uytdehaag F., Zegers B. J. M. 1979; Regulation of B cell activity in man: role of T cells. Immunological Reviews 45:3–39
     [Google Scholar]
  2. Borca M. V., Fernandez F. M., Sadir A. M., Braun M., Schudel A. A. 1986; Immune response to foot-and-mouth disease virus in a murine experimental model: effective thymus-independent primary and secondary reaction. Immunology 59:261–267
     [Google Scholar]
  3. Burns W. H., Billup L. C., Notkins A. L. 1975; Thymus dependence of virus antigen. Nature, London 256:654–655
     [Google Scholar]
  4. Calvert J., Kim M., Gathings W., Cooper M. 1983; Differentiation of B lineage cells from liver of neonatal mice: generation of immunoglobulin isotype diversity in vitro . Journal of Immunology 131:1693–1697
     [Google Scholar]
  5. Coffman R. L., Schrader B., Carty J., Mosmann T. R., Bond M. W. 1987; A mouse T cell product that preferentially enhances IgA production. I. Biologic characterization. Journal of Immunology 139:3685–3690
     [Google Scholar]
  6. Collen T., McCullough K. C., Doel T. R. 1984; Induction of antibody to foot-and-mouth disease virus in presensitised mouse spleen cell cultures. Journal of Virology 52:650–655
     [Google Scholar]
  7. Coutelier J-P., Van Der Logt J. T. M., Heessen F. W. A., Warnier G., Van Snick J. 1987; IgG2a restriction of murine antibodies elicited by viral infections. Journal of Experimental Medicine 165:64–69
     [Google Scholar]
  8. Dimarchi R., Brooke G., Gale C., Cracknell V., Doel T., Mowat N. 1986; Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science 232:639–641
     [Google Scholar]
  9. Good M. F., Maloy W. L., Lunde M. N., Margalit H., Cornette J. L., Smith G. L., Moss B., Miller L. H., Berzofsky J.A. 1987; Construction of a synthetic immunogen: use of a new T-cell epitope on malaria circumsporozoite protein. Science 235:1059–1062
     [Google Scholar]
  10. Hawrylowicz C. M., Klaus G. G. B. 1984; Activation and proliferation signals in mouse B cells. IV. Concanavalin A stimulates B cells to leave G0 but not to proliferate. Immunology 53:703–711
     [Google Scholar]
  11. Ishikawa M., Saito K. 1980; Congenitally athymic nude (nu/nu) mice have Thy-1-bearing immunocompetent helper T cells in their peritoneal cavity. Journal of Experimental Medicine 151:965–968
     [Google Scholar]
  12. Kärber G. 1931; Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Archiv für Experimented Pathologie und Pharmakologie 162:480–483
     [Google Scholar]
  13. Kishimoto T. 1985; Factors affecting B-cell growth and differentiation. Annual Review of Immunology 3:133–157
     [Google Scholar]
  14. Knudsen R. C., Groocock C. M., Andersen A. A. 1979; Immunity to foot-and-mouth disease virus in guinea pigs: clinical and immune responses. Infection and Immunity 24:787–792
     [Google Scholar]
  15. Mackowiak C., Lang C., Fontaine J., Camand R., Petermann H. G. 1962; Relationship between neutralising antibody titre and protection in animals immunised against foot-and-mouth disease. Annales de l’Institut Pasteur 103:252–261
     [Google Scholar]
  16. Melchers F., Andersson J. 1986; Factors controlling the B-cell cycle. Annual Review of Immunology 4:13–36
     [Google Scholar]
  17. Möller S. A., Danielsson L., Borrebaeck C. A. K. 1986; Concanavalin A-induced B-cell proliferation mediated by allogeneically derived helper factors. Immunology 57:387–393
     [Google Scholar]
  18. Mosmann T. R., Coffman R. L. 1987; Two types of mouse helper T-cell clone. Implications for immune regulation. Immunology Today 8:223–227
     [Google Scholar]
  19. Pay T. W. F., Hingley P. J. 1987; Correlation of 140S antigen dose with the serum neutralising antibody response and the level of protection induced in cattle by foot-and-mouth disease vaccines. Vaccine 5:60–64
     [Google Scholar]
  20. Platt H. 1959; The occurrence of pancreatic lesions in adult mice infected with the virus of foot-and-mouth disease. Virology 9:484–186
     [Google Scholar]
  21. Raff M. C. 1973; θ-bearing lymphocytes in nude mice. Nature, London 246:350–351
     [Google Scholar]
  22. Sedgewick J. D., Holt P. G. 1983; A solid-phase immunoenzymatic technique for the enumeration of specific antibody secreting cells. Journal of Immunological Methods 57:301–309
     [Google Scholar]
  23. Sharma R., Prasad S., Ahuja K. L., Rahman M. M., Kumar A. 1985; Cell mediated immune response following foot-and-mouth disease vaccination in buffalo calves. Acta virologica 29:509–513
     [Google Scholar]
  24. Snapper C. M., Paul W. E. 1987; Interferon-γ and B cell stimulatory factor-1 reciprocally regulate Ig isotype production. Science 236:944–947
     [Google Scholar]
  25. Taswell C. 1981; Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis. Journal of Immunology 126:1614–1619
     [Google Scholar]
  26. Uytdehaag F. G. C. M., Loggen H. G., Logtenberg T., Lichveld R. A., Van Steenis B., Van Asten J. A. A. M., Osterhaus A. D. M. E. 1985; Human peripheral blood lymphocytes from recently vaccinated individuals produce both type-specific and intertypic cross-reacting neutralising antibody on in vitro stimulation with one type of poliovirus. Journal of Immunology 135:3094–3101
     [Google Scholar]
  27. Wardley R. C., Chapman W. G., Garland A. J. M. 1979; A blastogenic test for foot-and-mouth disease virus. Journal of Hygiene 83:507–512
     [Google Scholar]
  28. Yuan D., Vitetta E. 1983; Structural studies of cell surface and secreted IgG in LPS-stimulated murine B cells. Molecular Immunology 20:367–375
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-70-2-395
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T Cell-dependent Induction of Antibody against Foot-and-Mouth Disease Virus in a Mouse Model
J. Gen. Virol. 70, 395 (1989); https://doi.org/10.1099/0022-1317-70-2-395
/content/journal/jgv/10.1099/0022-1317-70-2-395
/content/journal/jgv/10.1099/0022-1317-70-2-395
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