1887

Abstract

SUMMARY

The replication of flaviviruses results in the secretion of four virus-coded proteins into the extracellular environment. Three of these proteins, E, C and M (or pre-M), are found in purified virions. A fourth virus-specified extracellular protein which was not present in either the slowly sedimenting haemagglutinin particles or in virions is described. The relationship of this protein to the intracellular NS1 polypeptide was investigated along with its similarity to the soluble complement-fixing antigen (SCF) reported for mosquito-borne flaviviruses. The difference in the of NS1 and SCF is the result of additional glycosylation of SCF, mostly by the addition of fucose molecules. The synthesis of E and NS1 is sequential but their secretion is simultaneous, suggesting a role for NS1 in virion protein transport or virion release.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-2-335
1989-02-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/2/JV0700020335.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-2-335&mimeType=html&fmt=ahah

References

  1. Brandt W. E., Cardiff R. D., Russell P. K. 1970; Dengue virions and antigens in brain and serum of infected mice. Journal of Virology 6:500–506
    [Google Scholar]
  2. Cardiff R. D., McCloud T. G., Brandt W. E., Russell P. K. 1970; Molecular size and charge relationships of the soluble complement-fixing antigens of dengue viruses. Virology 41:569–572
    [Google Scholar]
  3. De Madrid A. T., Porterfield J. S. 1974; The flaviviruses (group B arboviruses): a cross-neutralization study. Journal of General Virology 23:91–96
    [Google Scholar]
  4. Gould E. A., Buckley A., Barrett A. D. T., Cammack N. 1986; Neutralizing (54K) and non-neutralizing (54K and 48K) monoclonal antibodies against structural and non-structural yellow fever virus proteins confer immunity in mice. Journal of General Virology 67:591–595
    [Google Scholar]
  5. Hambleton P., Stephenson J. R., Baskerville A., Wiblin C. N. 1983; Pathogenesis and immune response of vaccinated and unvaccinated rhesus monkeys to TBEV. Infection and Immunity 40:995–1003
    [Google Scholar]
  6. Heinz F. X., Kunz C. 1977; Characterization of TBEV and immunogenicity of its surface components in mice. Acta virologica 21:308–316
    [Google Scholar]
  7. Lavrova N. A. 1977; The dynamics of replication of infectious WNV and accumulation of its antigens in the blood and brain of experimentally infected mice. Voprosy virusologii 22:193–196
    [Google Scholar]
  8. Mason P. W., Mcada P. C., Dalrymple J. M., Fournier M. J., Mason T. L. 1987; Expression of Japanese encephalitis virus antigens in E. coli. Virology 158:361–372
    [Google Scholar]
  9. Phillpotts R. J., Stephenson J. R., Porterfield J. S. 1985; Antibody-dependent enhancement of tick-borne encephalitis virus infectivity. Journal of General Virology 66:1831–1837
    [Google Scholar]
  10. Phillpotts R. J., Stephenson J. R., Porterfield J. S. 1986; Passive protection of mice with monoclonal antibodies raised against tick-borne encephalitis virus. Archives of Virology 93:295–301
    [Google Scholar]
  11. Rai J., Gosh S. N. 1976; Studies on soluble complement fixing antigen of Japanese encephalitis virus. Indian Journal of Medical Research 64:981–991
    [Google Scholar]
  12. Rice C. M., Lenches E. M., Eddy S. R., Shin S. J., Sheets R. L., Strauss J. H. 1985; Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229:726–733
    [Google Scholar]
  13. Russell P. K., Brandt W. E., Dalrymple J. M. 1980; Chemical and antigenic structure of flaviviruses. In The Togaviruses503–529 Schlesinger R. W. London: Academic Press;
    [Google Scholar]
  14. Schlesinger J. J., Brandriss M. W., Monath T. P. 1983; Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition and immune precipitation of the virus envelope protein. Virology 125:8–17
    [Google Scholar]
  15. Schlesinger J. J., Brandriss M. W., Cropp C. B., Monath T. P. 1986; Protection against yellow fever in monkeys by immunization with yellow fever virus nonstructural NS1. Journal of Virology 60:1153–1155
    [Google Scholar]
  16. Schlesinger J. J., Brandriss M. W., Walsh E. E. 1987; Protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein NS1. Journal of General Virology 68:853–857
    [Google Scholar]
  17. Shapiro D., Brandt W. E., Cardiff R. D., Russell P. K. 1971; The proteins of Japanese encephalitis virus. Virology 44:108–124
    [Google Scholar]
  18. Smith G. W., Wright P. J. 1985; Synthesis of proteins and glycoproteins in dengue type 2 virus-infected Vero and Aedes albopictus cells. Journal of General Virology 66:559–571
    [Google Scholar]
  19. Speight G., Coia G., Parker M. D., Westaway E. G. 1988; Gene mapping and positive identification of the non-structural proteins NS2A, NS2B, NS3, NS4B and NS5 of the flavivirus Kunjin and their cleavage sites. Journal of General Virology 69:23–34
    [Google Scholar]
  20. Stephenson J. R., Hay A. J., Skehel J. J. 1977; Characterization of virus-specific messenger RNAs from avian fibroblasts infected with fowl plague virus. Journal of General Virology 36:237–248
    [Google Scholar]
  21. Stephenson J. R., Lee J. M., Wilton-Smith P. D. 1984; Antigenic variation among members of the tick-borne encephalitis complex. Journal of General Virology 65:81–89
    [Google Scholar]
  22. Stephenson J. R., Crooks A. J., Lee J. M. 1987; The synthesis of immunogenic polypeptides encoded by tick-borne encephalitis virus. Journal of General Virology 68:1307–1316
    [Google Scholar]
  23. Westaway E. G. 1987; Flavivirus replication strategy. Advances in Virus Research 33:45–90
    [Google Scholar]
  24. Winkler G., Randolph V. B., Cleaves G. R., Ryan T. E., Stollar V. 1988; Evidence that the mature form of the flavivirus nonstructural protein NS1 is a dimer. Virology 162:187–196
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-70-2-335
Loading
/content/journal/jgv/10.1099/0022-1317-70-2-335
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error