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The nucleotide sequence of the genomic RNA of narcissus mosaic virus (NMV) was deduced from a set of cDNA clones and by direct sequencing of RNA. The genome, with a length of 6955 nucleotides [excluding the 3′poly(A) tail], contains six open reading frames (ORFs) with the capacity to code for polypeptides of more than 10K, with M r values of 186284, 25845, 13998, 11059, 26097 and 10519. The first five of these putative proteins show considerable homology to similar proteins encoded by the RNAs of potato virus X (PVX, 6435 nucleotides) and white clover mosaic virus (WClMV, 5845 nucleotides). The sixth ORF is completely overlapped by the M r 26097 coat protein cistron and has some homology with a similar ORF in WClMV RNA. The difference in length of the RNAs of NMV, PVX and WClMV is due to a non-homologous domain of variable length in the central region of the 5′-proximal ORF of the three viruses.
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