1887

Abstract

SUMMARY

Infection of L-A9 cells with Marituba virus produces a severe inhibition of protein synthesis. This inhibition is temporally correlated with an increase in the intracellular Na concentration and a decrease in the intracellular K concentration. However in Marituba virus-infected cells the intracellular level of Na and K ions and protein synthesis remained unaltered. Incubation of both cell types at high NaCl concentration facilitated the translation of viral RNA whereas the cellular protein synthesis was inhibited. Using a hypotonic medium, the opposite was found. Results are discussed in terms of a possible involvement of these ions in the viral translational process.

Keyword(s): cations , MTBV and protein synthesis
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/content/journal/jgv/10.1099/0022-1317-70-12-3493
1989-12-01
2024-04-19
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References

  1. Alonso M. A., Carrasco L. 1981; Relationship between membrane permeability and the translations capacity of human HeLa cells studied by means of the ionophore nigericin. European Journal of Biochemistry 188:289–294
    [Google Scholar]
  2. Carrasco L. 1977; The inhibition of cell functions after viral infection: a proposed general mechanism. FEBS Letters 76:11–15
    [Google Scholar]
  3. Carrasco L., Smith A. E. 1976; Sodium ions and the shut-off of host cell protein synthesis by picornavirus. Nature., London 264:807–809
    [Google Scholar]
  4. Carvalho M. G. C., Frugulhetti I. C. P. P., Rebello M. A. 1986; Marituba (Bunyaviridae) virus replication in cultured Aedes albopictus cells and L-A9 cells. Archives of Virology 90:325–335
    [Google Scholar]
  5. Causey O. R., Causey C. E., Maroia O. M., Macedo D. G. 1961; The isolation of arthropod-borne viruses, including members of two hitherto undescribed serological groups, in the Amazon region of Brazil. American Journal of Tropical Medicine and Hygiene 10:227–249
    [Google Scholar]
  6. Cherney C. S., Wilhelm J. M. 1979; Differential translation in normal and adenovirus type 5-infected human cells and cell-free systems. Journal of Virology 30:533–542
    [Google Scholar]
  7. Elliott R. M., Wilkle M. 1986; Persistent infection of Aedes albopictus C6/36 cells by Bunyamwera virus. Virology 150:21–32
    [Google Scholar]
  8. Frugulhetti I. C. P. P., Soares M. C. M., Rebello M. A. 1983; Structural proteins of Marituba virus (Bunyaviridae). Intervirology 20:143–148
    [Google Scholar]
  9. Garry R. F., Vlug E. T., Bose H. R. 1982; Membrane-mediated alterations of intracellular Na+ and K+ in lytic virus-infected and retrovirus-transformed cells. Bioscience Reports 2:617–623
    [Google Scholar]
  10. Igarashi A. 1978; Isolation of a Singh’s Aedes albopictus cell clone sensitive to dengue and chikungunya virus. Journal of General Virology 40:531–544
    [Google Scholar]
  11. Kozak M. 1986; Bifunctional messenger RNAs in eukaryotes. Cell 47:481–483
    [Google Scholar]
  12. Lacal J. C., Carrasco L. 1982; Relationship between membrane integrity and the inhibition of host translation in virus-infected mammalian cells. Comparative studies of poliovirus. European Journal of Biochemistry 127:359–366
    [Google Scholar]
  13. Nair C. N. 1981; Monovalent cation metabolism and cytopathic effects of poliovirus infected HeLa cells. Journal of Virology 37:268–273
    [Google Scholar]
  14. Nuss D. L., Oppermann H., Koch G. 1975; Selective blockage of inhibition of host protein synthesis in RNA-virus infected cells. Proceedings of the National Academy of SciencesU.S.A 72:1258–1262
    [Google Scholar]
  15. Rebello M. A., Volkmer N., Frugulhetti I. C. P. P., Soares M. C. M. 1981; Macromolecular synthesis in L-A9 cells infected with Marituba virus. Archives of Virology 68:327–332
    [Google Scholar]
  16. Saborio J. L., Pong S. S., Koch G. 1974; Selective and reversible inhibition of initiation of protein synthesis in mammalian cells. Journal of Molecular Biology 85:195–211
    [Google Scholar]
  17. Schaefer A., Kuhne J., Zibirre R., Koch G. 1982; Poliovirus-induced alteration in HeLa cell membrane function. Journal of Virology 44:444–449
    [Google Scholar]
  18. Verani P., Nicoletti L., Marchi A. 1984; Establishment and maintenance of persistent infection by the Phlebovirus Toscana in Vero cells. Journal of General Virology 65:367–375
    [Google Scholar]
  19. Volkmer N., Rebello M. A. 1981; A plaque assay method for Marituba virus in mouse L-A9 cells. IRCS Medical Science: Membrane Biology 9:157–158
    [Google Scholar]
  20. Volkmer N., Soares M. C. M., Rebello M. A. 1983; Autointerference of Marituba virus (Bunyaviridae) in mouse L cells by defective interfering particles. Intervirology 20:108–113
    [Google Scholar]
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