Detection of Defective Genomes in Hepatitis A Virus Particles Present in Clinical Specimens Free

Abstract

SUMMARY

Hepatitis A virus (HAV) particles harbouring a physically defective RNA genome have been reported to occur in all HAV-infected cell culture systems analysed so far. The most prominent defects consist of three distinct overlapping deletions in the region of the HAV genome encoding the structural proteins. By probing for the endpoints of these deletions in RNA samples using S1 nuclease and exonuclease VII mapping, we obtained suggestive evidence for the existence also of defective genomes in HAV particles present in faecal specimens, in viraemic blood collected in the course of hepatitis A virus infection in man, as well as in the liver of an experimentally infected marmoset monkey. The deletions identified extend from nucleotide (nt) 1200 to nt 3820 and from nt 1200 to nt 3240 of the HAV genome. They are compatible with two of the deletions detected in particles grown in cell cultures and shown to interfere with the replication of standard hepatitis A virions.

Keyword(s): clinical specimens , DI particles and HAV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-12-3475
1989-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/12/JV0700123475.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-12-3475&mimeType=html&fmt=ahah

References

  1. Barrett A. D. T., Dimmock N. J. 1986; Defective interfering viruses and infections of animals. Current Topics in Microbiology and Immunology 128:55–84
    [Google Scholar]
  2. De Chastonay S., Siegl G. 1987; Replicative events in hepatitis A virus-infected MRC-5 cells. Virology 157:268–275
    [Google Scholar]
  3. Frösner G. G., Deinhardt F., Scheid R., Gauss-Muller V., Holmes N., Messelberger V., Siegl G., Alexander J. J. 1979; Propagation of human hepatitis A virus in a hepatoma cell line. Infection 7:303–306
    [Google Scholar]
  4. Hollinger F. B., Khan N. C., Oefinger P. E., Yawn D. H., Schmulen A. C., Dreesman G. R., Melnick J. L. 1983; Post-transfusion hepatitis type A. Journal of the American Medical Association 250:2313–2317
    [Google Scholar]
  5. Mcclure M. A., Perrault J. 1986; RNA virus genomes hybridize to cellular rRNAs and to each other. Journal of Virology 57:917–921
    [Google Scholar]
  6. NüescH J., Krech S., Siegl G. 1988; Detection and characterization of subgenomic RNAs in hepatitis A virus particles. Virology 165:419–427
    [Google Scholar]
  7. Provost P. J., Wolanski B. S., Miller W. J., Ittensohn O. L., Mcaleer W. J., Hilleman M. R. 1975; Physical, chemical and morphologic dimensions of human hepatitis A virus strain CR326 (38578). Proceedings of the Society for Experimental Biology and Medicine 148:532–539
    [Google Scholar]
  8. Schilt U., Burkhardt F., Siegl G. 1982; Virological and serological data of a hepatitis A outbreak in Wasen i.E., Switzerland. Experientia 38:1366
    [Google Scholar]
  9. Siegl G., De Chastonay J., Kronauer G. 1984; Propagation and assay of hepatitis A virus in vitro . Journal of Virological Methods 9:53–67
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-12-3475
Loading
/content/journal/jgv/10.1099/0022-1317-70-12-3475
Loading

Data & Media loading...

Most cited Most Cited RSS feed