Morphological Characteristics of Rice Stripe Virus Free

Abstract

SUMMARY

The morphological characteristics of particles of rice stripe virus (RSV) were examined. Each of four components (M1, M2, B and nB) of RSV, isolated by repeated cycles of sucrose density gradient centrifugation, contained circular filaments; the modal lengths were 510 nm for the M1 component, 610 nm for the M2 component, 840 nm for the B component and 2110 nm for the nB component. Each component was associated with a single-stranded species and a double-stranded species of RNA although a fifth component (the T component) did not form a distinct band after the density gradient centrifugation and was associated with circular filaments of 290 nm in length and a ssRNA species.

Keyword(s): circular filaments , RSV and tenuiviruses
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-12-3465
1989-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/12/JV0700123465.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-12-3465&mimeType=html&fmt=ahah

References

  1. Falk B. W., Tsai J. H. 1984; Identification of single- and double-stranded RNAs associated with maize stripe virus. Phytopathology 74:909–915
    [Google Scholar]
  2. Gingery R. E. 1987; The rice stripe virus group. In The Plant Viruses 4297–329 Milne R. G. New York: Plenum Press;
    [Google Scholar]
  3. Gingery R. E., Nault L. R., Bradfute O. E. 1981; Maize stripe virus: characteristics of a member of a new virus class. Virology 112:99–108
    [Google Scholar]
  4. Gingery R. E., Nault L. R., Yamashita S. 1983; Relationship between maize stripe virus and rice stripe virus. Journal of General Virology 64:1765–1770
    [Google Scholar]
  5. Hibino H., Usugi T., Omura T., Tsuchlzakl T., Shohara K., Iwasaki M. 1985; Rice grassy stunt virus: a planthopper-borne circular filament. Phytopathology 75:894–899
    [Google Scholar]
  6. Ishikawa K., Omura T., Tsuchizaki T. 1989; Association of double- and single-stranded RNAs with each four components of rice stripe virus. Annals of the Phytopathological Society of Japan (in press)
    [Google Scholar]
  7. Koganezawa H. 1977; Purification and properties of rice stripe virus. In Symposium on Virus Diseases of Tropical Crops, Tropical Agriculture Research Series no. 10151–154 Tropical Agriculture Research Center, Ministry of Agriculture and Forestry; Japan:
    [Google Scholar]
  8. Morales F. J., Niessen A. I. 1983; Association of spiral filamentous viruslike particles with rice hoja bianca. Phytopathology 73:971–974
    [Google Scholar]
  9. Omura T., Takahashi Y., Shohara K., Minobe Y., Tsuchizaki T., Nozu Y. 1986; Production of monoclonal antibodies against rice stripe virus for the detection of virus antigen in infected plants and viruliferous insects. Annals of the Phytopathological Society of Japan 52:270–277
    [Google Scholar]
  10. Pares R. D., Whitecross M. I. 1982; Gold-labelled antibody decoration (GLAD) in the diagnosis of plant viruses by immuno-electron microscopy. Journal of Immunological Methods 51:23–28
    [Google Scholar]
  11. Toriyama S. 1982; Characterization of rice stripe virus: a heavy component carrying infectivity. Journal of General Virology 61:187–195
    [Google Scholar]
  12. Toriyama S. 1983; Rice stripe virus. CMI/AAB Descriptions of Plant Viruses no. 269
    [Google Scholar]
  13. Toriyama S. 1987; Ribonucleic acid polymerase activity in filamentous nucleoproteins of rice grassy stunt virus. Journal of General Virology 68:925–929
    [Google Scholar]
  14. Toriyama S., Watanabe Y. 1989; Characterization of single- and double-stranded RNAs in particles of rice stripe virus. Journal of General Virology 70:505–511
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-12-3465
Loading
/content/journal/jgv/10.1099/0022-1317-70-12-3465
Loading

Data & Media loading...

Most cited Most Cited RSS feed