Ten Japanese field isolates of beet necrotic yellow vein virus (BNYVV) were transmitted to Tetragonia expansa by inoculation with sap from rootlets of sugar-beet seedlings, to which the virus had been transmitted by the fungus Polymyxa betae. RNA extracted from BNYVV particles obtained from the T. expansa leaves was analysed by agarose gel electrophoresis. Some isolates contained RNA-1 (7·1 kb), RNA-2 (4·8 kb), RNA-3 (1·85 kb) and RNA-4 (1·5 kb) and the others contained, in addition, RNA-5 (1·4 kb). Further isolates, derived from single lesions produced by these isolates, had a variety of RNA compositions. Some contained only RNA-1 and RNA-2. Others contained, in addition, RNA-3, RNA-4, RNA-5 or RNA-6 (1·0 kb), or combinations of two or three of these components. Such isolates generally maintained their RNA composition on further subculture, and their particles had length distributions corresponding to their RNA components. Isolates containing RNA-1 + 2 + 3 caused yellow or strongly chlorotic local lesions in T. expansa, Beta vulgaris, B. macrocarpa and Chenopodium quinoa, and caused systemic stunting and yellow mosaic in B. macrocarpa and, occasionally, in B. vulgaris. In contrast, isolates containing RNA-1 + 2 + 4 or 1 + 2 + 5 induced chlorotic lesions, those containing RNA-1 + 2 + 6 or 1 + 2 induced faint chlorotic lesions, and none of these isolates easily infected B. macrocarpa systemically. Isolates containing different combinations of RNA-3,-4 and -5 induced more severe symptoms than those containing a single RNA. Such synergistic effects occurred between RNA-3 and RNA-4 or RNA-5, or between RNA-4 and RNA-5 or RNA-6, but not between RNA-3 and RNA-6, or between RNA-5 and RNA-6. These small RNA species therefore contain the genetic determinant(s) for lesion type and for ability to infect B. vulgaris and B. macrocarpa systemically. RNA-1 and RNA-2 are viral genome components. The other RNA components have some characteristics of viral satellite nucleic acids but they may not all be dispensable if the BNYVV isolates are to survive in nature.
AbeH.,
UiT.1986; Host range of Polymyxa betae Keskin strains in rhizomania-infested soils of sugar beet in Japan. Annals of the Phytopathological Society of Japan 52:394–403
BouzoubaaS.,
GuilleyH.,
IonardG.,
RichardsK.,
PutzC.1985; Nucleotide sequence analysis of RNA-3 and RNA-4 of beet necrotic yellow vein virus, isolates F2 and G1. Journal of General Virology 66:1553–1564
BrosiusI.,
PalmerM. L.,
KennedyP. J.,
NollerH. F.1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli
. Proceedings of the National Academy of SciencesU.S.A 75:4801–4805
BrosiusJ.,
DullT. J.,
NollerH. F.1980; Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli
. Proceedings of the National Academy of SciencesU.S.A 77:201–204
BurgermeisterW.,
KoenigR.,
WeichH.,
SebaldW.,
LesemannD. E.1986; Diversity of the RNAs in thirteen isolates of beet necrotic yellow vein virus in Chenopodium quinoa detected by means of cloned cDNAs. Journal of Phytopathology 115:229–242
DahlbergA. E.,
DingmanC. W.,
PeacockA. C.1969; Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels. Journal of Molecular Biology 41:139–147
GoeletP.,
LomonossoffG. P.,
ButlerP. I. G.,
AkamM. E.,
GaitM. J.,
KarnJ.1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of SciencesU.S.A 79:5818–5822
KiguchiT.,
TamadaT.,
SaitoM.,
HaradaT.,
UgakiM.,
MotoyoshiF.1988; Comparison of four small RNAs of beet necrotic yellow vein virus in association with symptom expression. Fifth International Congress of Plant PathologyKyoto, Japan, 1988 Abstracts of Papers 453
KoenigR.,
BurgermeisterW.,
WeichH.,
SebaldW.,
KotheC.1986; Uniform RNA patterns of beet necrotic yellow vein virus in sugarbeet roots, but not in leaves from several plant species. Journal of General Virology 67:2043–2046
KuszalaM.,
ZieglerV.,
BouzoubaaS.,
RichardsK.,
PutzC.,
GuilleyH.,
JonardG.1986; Beet necrotic yellow vein virus: different isolates are serologically similar but differ in RNA composition. Annals of Applied Biology 109:155–162
LehrachH.,
DiamondD.,
WozneyJ. M.,
BoedtkerH.1977; RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743–4751
RichardsK.,
JonardG.,
GuilleyH.,
ZieglerV.,
PutzC.1985; In vitro translation of beet necrotic yellow vein virus RNA and studies of sequence homology among the RNA species using cloned cDNA probes. Journal of General Virology 66:345–350
SaitoM.,
HaradaT.,
KiguchiT.,
TamadaT.1988; Cloning of cDNA to beet necrotic yellow vein virus and its use for the RNA detection by photobiotin-labelling. Fifth International Congress of Plant PathologyKyoto, Japan 1988: Abstracts of Papers 45
TamadaT.,
BabaT.1973; Beet necrotic yellow vein virus from rhizomania-affected sugar beet in Japan. Annals of the Phytopathological Society of Japan 39:325–332
TamadaT.,
AbeH.,
SaitoM.,
KiguchiT., &. HaradaT.1988; The role of beet necrotic yellow vein virus RNA species in symptom expression and fungus transmission. Fifth International Congress of Plant PathologyKyoto, Japan 1988: Abstracts of Papers 31