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1887

Journal of General Virology header logo Journal of General Virology

Volume 70, Issue 12

Asynchronous Mixed Infection of with Bluetongue Virus Serotypes 10 and 17 No Access

Abstract

SUMMARY

(Diptera: Ceratopogonidae) the primary vector of bluetongue virus (BTV) in the U.S.A. were asynchronously mixedly infected with two BTV serotypes (BTV-10 and BTV-17); flies first ingested a blood meal that contained BTV-17 and 1, 3, 5, 7, and 9 days later selected flies ingested a second blood meal that contained BTV-10. Control flies ingested each parental virus separately, or both viruses simultaneously, in a single blood meal. Electrophoretic analysis of progeny virus clones indicated that superinfection with BTV-10 occurred when the flies ingested the second virus 1, 3 and 5 days post-initial infection. Parental BTV-17 and reassortant virus clones were isolated from these flies, but parental BTV-10 virus was not isolated from any flies. Reassortant clone frequencies were 67%, 71% and 17% when superinfection occurred on days 1, 3 and 5 after initial infection, respectively, as compared to 48% for simultaneously infected flies. Only parental BTV-17 clones were isolated from flies that ingested the second virus on days 7 and 9 after initial BTV-17 infection. The results indicated that interference to superinfection occurred in by 5 days and flies were refractory to superinfection by 7 days post-initial infection. Analysis of segregation of the parental origin of genome segments in the reassortant clones indicated selection against most segments of BTV-10 parental origin. This occurred both in individual flies and in individual groups. The fact that readily fed on a second blood meal and their ability to produce new viral genotypes suggested that these vectors are highly permissive hosts for evolution of BTV by genome reassortment.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): asynchronous vector infection , bluetongue virus , genome reassortment and interference
© Society for General Microbiology 1989
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1989-12-01
2025-04-30
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/content/journal/jgv/10.1099/0022-1317-70-12-3355
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Asynchronous Mixed Infection of Culicoides variipennis with Bluetongue Virus Serotypes 10 and 17
J. Gen. Virol. 70, 3355 (1989); https://doi.org/10.1099/0022-1317-70-12-3355
/content/journal/jgv/10.1099/0022-1317-70-12-3355
/content/journal/jgv/10.1099/0022-1317-70-12-3355
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