@article{mbs:/content/journal/jgv/10.1099/0022-1317-70-12-3317, author = "Klavinskis, Linda S. and Geckeler, Robert and Oldstone*, Michael B. A.", title = "Cytotoxic T Lymphocyte Control of Acute Lymphocytic Choriomeningitis Virus Infection: Interferon γ, but Not Tumour Necrosis Factor α, Displays Antiviral Activity in vivo", journal= "Journal of General Virology", year = "1989", volume = "70", number = "12", pages = "3317-3325", doi = "https://doi.org/10.1099/0022-1317-70-12-3317", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-70-12-3317", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "interferon", keywords = "cytolysis", keywords = "clearance", keywords = "CTL", keywords = "tumour necrosis factor", abstract = "SUMMARY Virus-specific cytotoxic T lymphocytes (CTL) mediate their antiviral activity either by direct lysis of infected cells, or by the release of soluble lymphokines, or by a combination of the two. We have examined the role played by interferon-gamma (IFN-γ) and tumour necrosis factor (TNFα) in virus clearance. In vitro the amount of IFN-γ synthesized by some lymphocytic choriomeningitis virus-specific H-2-restricted CTL clones was quantitatively too small to correlate with a direct antiviral activity in vivo. However, treatment of mice with a neutralizing monoclonal antibody to IFN-γ significantly inhibited the clearance of virus from the spleens of acutely infected mice given adoptive transfers of immune spleen cells. Additionally, mice treated with exogenous recombinant murine IFN-γ 24 h before or at the same time as virus inoculation showed reduced virus titres in their spleens. Hence, IFN-γ displayed a direct antiviral effect in vivo. In contrast, treatment of mice with recombinant TNFα had no effect on virus clearance and thus TNFα is unlikely to play a significant role in this acute viral infection.", }