1887

Abstract

SUMMARY

Virus-specific cytotoxic T lymphocytes (CTL) mediate their antiviral activity either by direct lysis of infected cells, or by the release of soluble lymphokines, or by a combination of the two. We have examined the role played by interferon-gamma (IFN-) and tumour necrosis factor (TNF) in virus clearance. the amount of IFN- synthesized by some lymphocytic choriomeningitis virus-specific H-2-restricted CTL clones was quantitatively too small to correlate with a direct antiviral activity . However, treatment of mice with a neutralizing monoclonal antibody to IFN- significantly inhibited the clearance of virus from the spleens of acutely infected mice given adoptive transfers of immune spleen cells. Additionally, mice treated with exogenous recombinant murine IFN- 24 h before or at the same time as virus inoculation showed reduced virus titres in their spleens. Hence, IFN- displayed a direct antiviral effect . In contrast, treatment of mice with recombinant TNF had no effect on virus clearance and thus TNF is unlikely to play a significant role in this acute viral infection.

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1989-12-01
2022-01-24
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