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Journal of General Virology header logo Journal of General Virology

Volume 70, Issue 12

Phosphorylation of Adenovirus DNA-binding Protein No Access

Abstract

SUMMARY

Evidence is presented here which indicates that the adenovirus DNA-binding protein (DBP) is phosphorylated at a tyrosine residue early in infection. This was suggested by the discovery that a proportion of the label in P-labelled DBP was resistant to alkali, and was substantiated by acid hydrolysis of DBP immunoprecipitates and by immunoblotting with a monoclonal antibody against phosphotyrosine. Treatment of [S] methionine-labelled DBPs with chymotrypsin produced fragments of apparent 45K and 39K whereas digestion of P-labelled DBP resulted in fragments of 45K and 26K. Consideration of the distribution of P label and its alkali stability in these fragments suggested that chymotrypsin cleaved populations of DBP at different sites depending on their phosphorylation states. The conservation, in all of the seven adenovirus serotypes sequenced, of a tyrosine residue (at amino acid 195 in adenovirus type 2) together with its surrounding residues, suggests that phosphorylation/dephosphorylation at this tyrosine residue may be important in various functions ascribed to the DBP.

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Keyword(s): adenovirus , DNA-binding protein , phosphorylation and phosphotyrosine
© Society for General Microbiology 1989
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1989-12-01
2025-12-11

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References

  1. Anderson C. W., Hardy M. M., Dunn J. J., Klessig D. F. 1983; Independent, spontaneous mutants of adenovirus type 2 – simian virus 40 hybrid AD2+ ND3 that grow efficiently in monkey cells possess identical mutations in the adenovirus type 2 DNA-binding protein gene. Journal of Virology 48:31–39
     [Google Scholar]
  2. Antoniw J. F., Cohen P. 1976; Separation of two phosphorylase kinase phosphatases from rabbit skeletal muscle. European Journal of Biochemistry 68:45–54
     [Google Scholar]
  3. Ariga H., Klein A., Levine A., Horwitz M. 1980; A cleavage product of the adenoviral DNA binding protein is active in DNA replication in vitro . Virology 101:307–310
     [Google Scholar]
  4. Brough D. E., Rice S. A., Sell S. I., Klessig D. F. 1985; Restricted changes in the adenovirus DNA-binding protein that lead to extended host range or temperature-sensitive phenotypes. Journal of Virology 55:206–212
     [Google Scholar]
  5. Cleghon V. G., Klessig D. F. 1986; Association of the adenovirus DNA-binding protein with RNA both in vitro and in vivo . Proceedings of the National Academy of SciencesU.S.A 83:8947–8951
     [Google Scholar]
  6. Cleghon V. G., Voelkerding K., Morin N., Delsert C., Klessig D. F. 1989; Isolation and characterisation of a viable adenovirus mutant defective in nuclear transport of the DNA-binding protein. Journal of Virology 63:2289–2299
     [Google Scholar]
  7. Enomoto T., Lichy J. H., Ikeda J. E., Hurwitz J. 1981; Adenovirus DNA replication in vitro: purification of the terminal protein in a functional form. Proceedings of the National Academy of SciencesU.S.A 78:6779–6783
     [Google Scholar]
  8. Hamaguchi M., Grandori C., Hanafusa H. 1988; Phosphorylation of celular proteins in Rous sarcoma virus-infected cells: analyses by the use of phosphotyrosine antibodies. Molecular Cell Biology 8:3035–3042
     [Google Scholar]
  9. Hay R. T., Russell W. C. 1989; Recognition mechanisms in the synthesis of animal virus DNA. Biochemical Journal 258:3–16
     [Google Scholar]
  10. Hunter T., Cooper J. A. 1985; Protein-tyrosine kinases. Annual Review of Biochemistry 54:897–930
     [Google Scholar]
  11. Hunter T., Sefton B. M. 1980; Transforming gene product of Rous sarcoma virus phosphorylates tyrosine. Proceedings of the National Academy of SciencesU.S.A 77:1311–1315
     [Google Scholar]
  12. Hunter T., Sefton B. M. 1983; Detection and quantification of phosphotyrosine in proteins. Methods in Enzymology 99:387–402
     [Google Scholar]
  13. Kamps M. P., Sefton B. M. 1988; Identification of multiple novel polypeptide substrates of the v-src v-yes v-fps and v-erb-B oncogenic tyrosine protein kinases utilising antisera against phosphotyrosine. Oncogene 2:305–315
     [Google Scholar]
  14. Kitchingman G. R. 1985; Sequence of the DNA-binding protein of a human subgroup E adenovirus (type 4): comparisons with subgroup A (type 12), subgroup B (type 7), and subgroup C (type 5). Virology 156:90–101
     [Google Scholar]
  15. Klein H., Maltzman W., Levine A. I. 1979; Structure-function relationships of the adenovirus DNA-binding protein. Journal of Biological Chemistry 254:11051–11060
     [Google Scholar]
  16. Leader D. P., Katan M. 1988; Viral aspects of protein phosphorylation. Journal of General Virology 69:1441–1464
     [Google Scholar]
  17. Linne T., Philipson L. 1980; Further characterisation of the phosphate moiety of the adenovirus type 2 DNA-binding protein. European Journal of Biochemistry 103:259–270
     [Google Scholar]
  18. Linne T., Jornvall H., Philipson L. 1977; Purification and characterisation of the phosphorylated DNA-binding protein from adenovirus type 2 infected cells. European Journal of Biochemistry 76:481–490
     [Google Scholar]
  19. Rice S. A., Klessig D. F. 1984; The function(s) provided by the adenovirus-specified, DNA-binding protein required for viral late gene expression is independent of the role of the protein in viral DNA replication. Journal of Virology 49:35–49
     [Google Scholar]
  20. Russell W. C., Blair G. E. 1977; Polypeptide phosphorylation in adenovirus-infected cells. Journal of General Virology 34:19–35
     [Google Scholar]
  21. Russell W. C., Precious B. 1982; Nucleic acid-binding properties of adenovirus structural polypeptides. Journal of General Virology 63:69–79
     [Google Scholar]
  22. Russell W. C., Hayashi K., Sanderson P. J., Pereira H. G. 1967; Adenovirus antigens - a study of their properties and sequential development in infection. Journal of General Virology 1:495–507
     [Google Scholar]
  23. Russell W. C., Patel G., Precious B., Sharp I., Gardner P. S. 1981; Monoclonal antibodies against adenovirus type 5: preparation and preliminary characterization. Journal of General Virology 56:393–408
     [Google Scholar]
  24. Schechter N. M., Davies W., Anderson C. W. 1980; Adenovirus-coded deoxyribonucleic acid binding protein: isolation, physical properties, and effects of proteolytic digestion. Biochemistry 19:2802–2810
     [Google Scholar]
  25. Seiberg M., Aloni Y., Levine A. J. 1989; The adenovirus type 2 DNA-binding protein interacts with the major late promoter attenuated RNA. Journal of Virology 63:1134–1141
     [Google Scholar]
  26. Swarup G., Cohen S., Garbers D. L. 1982; Inhibition of membrane phosphotyrosyl-protein phosphatase activity by vanadate. Bichemical and Biophysical Research Communications 107:1104–1109
     [Google Scholar]
  27. Tsernoglou D., Tsugita A., Tucker A. D., Van Der Vliet P. C. 1985; Characterisation of the chymotryptic core of the adenovirus DNA-binding protein. FEBS Letters 188:248–252
     [Google Scholar]
  28. Van Der Vliet P. C., Keegstra W., Jansz H. S. 1978; Complex formation between the adenovirus type 5 DNA-binding protein and single-stranded DNA. European Journal of Biochemistry 86:389–398
     [Google Scholar]
  29. Vos H. L., Van Der Lee F. M., Feemst A. M. C. B., Van Loon A. E., Sussenbach J. S. 1988; The genes encoding the DNA-binding protein and the 23K protease of adenovirus type 40 and 41. Virology 163:1–10
     [Google Scholar]
  30. Winters W. D., Russell W. C. 1971; Studies on the assembly of adenovirus in vitro . Journal of General Virology 10:181–194
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-70-12-3249
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Phosphorylation of Adenovirus DNA-binding Protein
J. Gen. Virol. 70, 3249 (1989); https://doi.org/10.1099/0022-1317-70-12-3249
/content/journal/jgv/10.1099/0022-1317-70-12-3249
/content/journal/jgv/10.1099/0022-1317-70-12-3249
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