1887

Abstract

Summary

Unambiguous assignment of restriction enzyme patterns to six individual serotypes of human rhinovirus was accomplished after amplification of a 380 bp DNA fragment derived from the 5′ non-coding region. This was possible even though serotypes 1A and 1B and serotypes 2 and 49 differed only at 10 and 15 positions respectively. The method utilizes the conserved and variable components of this part of the genome and provides the basis for a simple and rapid method for typing of human rhinoviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-11-3111
1989-11-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/11/JV0700113111.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-11-3111&mimeType=html&fmt=ahah

References

  1. Bruce C. B., Al-Nakib W., Tyrrell D. A. J., Almond J. W. 1988; Synthetic oligonucleotides as diagnostic probes. Lancet 8601:53
    [Google Scholar]
  2. Callahan P. L., Mizutani S., Colonno R. J. 1985; Molecular cloning and complete sequence determination of RNA sequence of human rhinovirus type 14. Proceedings of the National Academy of SciencesU.S.A 82732–736
    [Google Scholar]
  3. Cooney M. K., Fox J. P., Kenney G. E. 1982; Antigenic groupings of 90 rhinovirus serotypes. Infection And Immunity 37:642–647
    [Google Scholar]
  4. Duechler M., Skern T., Sommergruber W., Neubauer CH., Gruendler P., Fogy I., Blaas D., Kuechler E. 1987; Evolutionary relationships within the human rhinovirus genus; comparison of serotypes 89, 2 and 14. Proceedings of the National Academy of SciencesU.S.A 842605–2609
    [Google Scholar]
  5. Gama R. E., Hughes P. J., Bruce C. B., Stanway G. 1988; Polymerase chain reaction amplification of rhinovirus nucleic acids from clinical material. Nucleic Acids Research 16:9346
    [Google Scholar]
  6. Hamparian V. V., Colonno R. J., Cooney M. K., Dick E. C., Gwaltney J. M. JR, Hughes J. H., Jordan W. S., Kapikian A. Z., Mogabgab W. J., Monto A., Philips C. A., Rueckert R. R., Schieble J. H., Stott E. J., Tyrrell D. A. J. 1987; A collaborative report: Rhinoviruses – extension of the numbering system from 89 to 100. Virology 159:191–192
    [Google Scholar]
  7. Hughes P. J., North C., Jellis C. H., Minor P. D., Stanway G. 1988; The nucleotide sequence of human rhinovirus 1B: molecular relationships within the rhinovirus genus. Journal of General Virology 69:49–58
    [Google Scholar]
  8. Isono K. 1982; Computer programs to analyze DNA and amino acid sequence data. Nucleic Acids Research 10:85–89
    [Google Scholar]
  9. Kellner G., Popow-kraupp T., Kundi M., Binder C., Wallner H., Kunz C. 1988; Contribution of rhinoviruses to respiratory infections in childhood: a prospective study in a mainly hospitalized infant population. Journal of Medical Virology 25:455–469
    [Google Scholar]
  10. Kogan S. C., Doherty M., Gitschier J. 1987; An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences. New England Journal of Medicine 317:985–990
    [Google Scholar]
  11. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Minor P. D., Dunn G. 1988; The effect of sequences in the 5′ non-coding region on the replication of polioviruses in the human gut. Journal of General Virology 69:1091–1096
    [Google Scholar]
  13. Ou C-Y., Kwok S., Mitchell S. W., Mack D., Sninsky J., Krebs J., Feorino P., Warfield D., Schochetman G. 1988; DNA amplification for direct detection of HIV-1 DNA of peripheral blood mononuclear cells. Science 239:295–297
    [Google Scholar]
  14. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  15. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of SciencesU.S.A 745463–5467
    [Google Scholar]
  16. Skern T., Sommergruber W., Blaas D., Gruendler P., Fraundorfer F., Pieler C., Fogy I., Kuechler E. 1985; Human rhinovirus 2: complete nucleotide sequence and proteolytic processing signals in the capsid protein region. Nucleic Acids Research 13:2111–2126
    [Google Scholar]
  17. Stanway G., Hughes P. J., Mountford R. C., Minor P. D., Almond J. W. 1984; The complete nucleotide sequence of a common cold virus: human rhinovirus 14. Nucleic Acids Research 12:7859–7875
    [Google Scholar]
  18. Stott E. J., Killington R. A. 1972; Rhinoviruses. Annual Review of Microbiology 26:503–525
    [Google Scholar]
  19. Torgersen H., Blaas D., Skern T. 1989; Low cost apparatus for primer-directed DNA amplification. Analytical Biochemistry 176:33–35
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-70-11-3111
Loading
/content/journal/jgv/10.1099/0022-1317-70-11-3111
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error