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Apple chlorotic leaf spot virus (ACLSV) RNA was translated in a rabbit reticulocyte lysate. Two major polypeptides of M r 105000 (105K) and 51 K, and some less prominent polypeptides (88K, 66K, 28K and 23K) were produced. The 23K polypeptide was identified as the coat protein on the basis of its electrophoretic mobility and serological reaction to virus antiserum. Time course and immuno-precipitation experiments suggested that the 23K polypeptide might be generated from the 28K polypeptide by post-translational cleavage.
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