1887

Abstract

Summary

Apple chlorotic leaf spot virus (ACLSV) RNA was translated in a rabbit reticulocyte lysate. Two major polypeptides of 105000 (105K) and 51 K, and some less prominent polypeptides (88K, 66K, 28K and 23K) were produced. The 23K polypeptide was identified as the coat protein on the basis of its electrophoretic mobility and serological reaction to virus antiserum. Time course and immuno-precipitation experiments suggested that the 23K polypeptide might be generated from the 28K polypeptide by post-translational cleavage.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-11-3051
1989-11-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/11/JV0700113051.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-11-3051&mimeType=html&fmt=ahah

References

  1. Bem F., Murant A. F. 1979; Comparison of particle properties of heracleum latent and apple chlorotic leaf spot viruses. Journal of General Virology 44:817–826
    [Google Scholar]
  2. Dougherty W. G., Hiebert E. 1980; Translation of potyvirus RNA in a rabbit reticulocyte lysate: identification of nuclear inclusion proteins as products of tobacco etch virus RNA translation and cylindrical inclusion protein as a product of the potyvirus genome. Virology 104:174–182
    [Google Scholar]
  3. Dougherty W. G., Hiebert E. 1985; Genome structure and gene expression of plant RNA viruses. In Molecular Plant Virology II Replication and Gene Expression 23–81 Davies J. W. Boca Raton: CRC Press;
    [Google Scholar]
  4. Forster A. C, McInnes J. L., Skingle D. C., Symons R. H. 1985; Nonradioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent. Photobiotin. Nucleic Acids Research 13:745–761
    [Google Scholar]
  5. Francki R. I. B., Milne R. G., Hatta T. 1985; Closterovirus group. In Atlas of Plant Viruses II219–234 Boca Raton: CRC Press;
    [Google Scholar]
  6. Habili N., Mcinnes J. L., Symons R. H. 1987; Nonradioactive, photobiotin-labelled DNA probes for the routine diagnosis of barley yellow dwarf virus. Journal of Virological Methods 16:225–237
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  8. Lister R. M. 1970; Apple chlorotic leaf spot virus. CMI/AAB Descriptions of Plant Viruses30
    [Google Scholar]
  9. Lister R. M., Bar-Joseph M. 1981; Closteroviruses. In Handbook of Plant Virus Infections and Comparative Diagnosis809–944 Kurstak E. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  10. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Németh M. 1986 Virus, Mycoplasma and Rickettsia Diseases of Fruit Trees Akadémiai KiadÓ;
    [Google Scholar]
  12. Shirako Y., Ehara Y. 1986; Comparison of the in vitro translation products of wild-type and a deletion mutant of soil-borne wheat mosaic virus. Journal of General Virology 67:1237–1245
    [Google Scholar]
  13. yanase H. 1974; Studies on apple latent viruses in Japan. Bulletin of the Fruit Tree Research Station Japan, Series C1:47–109
    [Google Scholar]
  14. Yanase H., Yamaguchi A., Mink G. I., Sawamura K. 1979; Back transmission of apple chlorotic leafspot virus (type strain) to apple and production of apple topworking disease symptoms in Maruba Kaido (Malus prunifolia Borkh. var ringo Asami). Annals of the Phytopathological Society of Japan 45:369–374
    [Google Scholar]
  15. Yoshikawa N., Takahashi T. 1988; Properties of RNAs and proteins of apple stem grooving and apple chlorotic leaf spot viruses. Journal of General Virology 69:241–245
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-11-3051
Loading
/content/journal/jgv/10.1099/0022-1317-70-11-3051
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error