1887

Abstract

Summary

Complementary DNA copies of the genomic RNA of melon necrotic spot virus (MNSV) have been cloned and the region deduced to encode the coat protein has been sequenced. The putative coat protein coding region, located near the 3′ end of the genome, consists of 1170 nucleotides and has the potential to encode a 390 amino acid protein of 41840. Our data show that although MNSV is a carmovirus, its coat protein more closely resembles those of the tombusviruses than those of the carmoviruses sequenced to date, in both the extent of sequence similarity and in the length of the random/arm and protruding domains of the coat protein. Furthermore, dot matrix comparisons revealed sequence similarity between the coat protein protruding domains of MNSV and the cucumber necrosis tombusvirus. This similarity may be involved in one or more of the biological properties these two viruses share, such as the ability to infect cucumbers naturally and to be transmitted by the soil-inhabiting fungus .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-11-3033
1989-11-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/11/JV0700113033.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-11-3033&mimeType=html&fmt=ahah

References

  1. Alwine J. C., Kemp D. J., Stark G. R. 1977; Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proceedings of the National Academy of SciencesU.S.A. 745350–5354
    [Google Scholar]
  2. Bailey J. M., Davidson N. 1976; Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry 70:75–85
    [Google Scholar]
  3. Barr P. J., Thayer R. M., Laybourn P., Najarian R. C., Seela F., Tolan D. R. 1986; 7-Deaza-2′- deoxyguanosine-5′-triphosphate: enhanced resolution in M13 dideoxy sequencing. BioTechniques 4:428–432
    [Google Scholar]
  4. Bos L., Van dorst H. J. M., Huttinga H., Maat D. Z. 1984; Further characterization of melon necrotic spot virus causing severe disease in glasshouse cucumbers in the Netherlands and its control. Netherlands Journal of Plant Pathology 90:55–69
    [Google Scholar]
  5. Carrington J. C., Morris T. J. 1985; Characterization of the cell-free translation products of carnation mottle virus genomic and subgenomic RNAs. Virology 144:1–10
    [Google Scholar]
  6. Carrington J. C., Morris T. J., Stockley P. G., Harrison S. C. 1987; Structure and assembly of turnip crinkle virus. IV. Analysis of the coat protein gene and implications of the subunit primary structure. Journal of Molecular Biology 194:265–276
    [Google Scholar]
  7. Dayhoff M. O., Eck R. V., Park C. M. 1969; A model of evolutionary change in proteins. In Atlas of Protein Sequence and Structure 475–83 Dayhoff M. O. Silver Spring: National Biomedical Research Foundation;
    [Google Scholar]
  8. Dias H. F., Mckeen C. D. 1972; Cucumber necrosis virus. CMI/AAB Descriptions of Plant Viruses82
    [Google Scholar]
  9. Gergen J. P., Stern R. H., Wensink P. C. 1979; Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Research 7:2115–2136
    [Google Scholar]
  10. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  11. Guilley H., Carrington J. C., Balazs E., Jonard G., Richards K., Morris T. J. 1985; Nucleotide sequence and genome organization of carnation mottle virus RNA. Nucleic AcidsResearch 13:6663–6677
    [Google Scholar]
  12. Harrison B. D. 1987; Plant virus transmission by vectors: mechanisms and consequences. In Symposia of the Society for General Microbiology 40Molecular Basis of Virus Disease319–344 Russell W. C., Almond J. W. New York & Cambridge: Cambridge University Press;
    [Google Scholar]
  13. Harrison S. C., Olson A. J., Schutt C. E., Winkler F. K. 1978; Tomato bushy stunt virus at 2.9 Å resolution. Nature London: 276368–373
    [Google Scholar]
  14. Hibi T., Furuki I. 1985; Melon necrotic spot virus. CMI/ AAB Descriptions of Plant Viruses302
    [Google Scholar]
  15. Hillman B. I., Hearne P., Rochon D., Morris T. J. 1989; Organization of tomato bushy stunt genome: characterization of the coat protein gene and the 3′ terminus. Virology 169:42–50
    [Google Scholar]
  16. Hogle J. M., Maeda A., Harrison S. C. 1986; Structure and assembly of turnip crinkle virus. I. X-ray crystallographic structure analysis at 3.2 Å resolution. Journal of Molecular Biology 191:625–638
    [Google Scholar]
  17. Hopper P., Harrison S. C., Sauer R. T. 1984; Structure of tomato bushy stunt virus. V. Coat protein sequence determination and its structural implications. Journal of Molecular Biology 177:701–713
    [Google Scholar]
  18. Koenig R., Kunze L. 1982; Identification of tombusvirus isolates from cherry in southern Germany as petunia asteroid mosaic virus. Phytopathologische Zeitschrift 103:361–368
    [Google Scholar]
  19. Korneluk R. G., Quan F., Gravel R. A. 1985; Rapid and reliable dideoxy sequencingof double-stranded DNA. Gene 40:317–323
    [Google Scholar]
  20. Lutcke H. A., Chow K. C., Mickel F. S., Moss K. A., Kern H. F., Scheele G. A. 1987; Selection of AUG initiation codons differs in plants and animals. EMBO Journal 6:43–48
    [Google Scholar]
  21. Martelli G. P., Gallitelli D., Russo M. 1988; Tombusviruses. In The Plant Viruses 313–72 Koenig R. New York: Plenum Press;
    [Google Scholar]
  22. Martin R. 1987; Overcoming DNA sequencing artifacts: stops and compressions. Focus 98–9 Gaithersburg: Bethesda Research Laboratories;
    [Google Scholar]
  23. Morris T. J., Carrington J. C. 1988; Carnation mottle virus and viruses with similar properties. In The Plant Viruses 373–112 Koenig R. New York: Plenum Press;
    [Google Scholar]
  24. Morris T. J., Dodds J. A. 1979; Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858
    [Google Scholar]
  25. Needleman S. B., Wunsch C. D. 1970; General method applicable to the search for similarities in the amino acid sequence of two proteins. Journal of Molecular Biology 48:443–453
    [Google Scholar]
  26. Pustell J., Kafatos F. C. 1984; Convenient and adaptable package of computer programs for DNA and protein sequence management, analysis and homology determination. Nucleic Acids Research 12:643–655
    [Google Scholar]
  27. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  28. Riviere C. J., Rochon D. M. 1988; Sequence and organization of the melon necrotic spot virus genome. Canadian Journal of Plant Pathology (abstract) 10:372
    [Google Scholar]
  29. Rochon D., Tremaine J. H. 1988; Cucumber necrosis virus is a member of the tombusvirus group. Journal of General Virology 69:395–400
    [Google Scholar]
  30. Rochon D. M., Tremaine J. H. 1989; Complete nucleotide sequence of the cucumber necrosis virus genome. Virology 169:251–259
    [Google Scholar]
  31. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of SciencesU.S.A. 745463–5467
    [Google Scholar]
  32. Sippel A. 1973; Purification and characterization of adenosinetriphosphate : ribonucleic acid adenyl transferase from Escherichia coli. European Journal of Biochemistry 37:31–34
    [Google Scholar]
  33. Staden R. 1982; An interactive graphics program for comparing and aligning nucleic acid andamino acid sequences. Nucleic Acids Research 10:2951–2961
    [Google Scholar]
  34. Tabor S., Richardson C. C. 1987; DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proceedings of the National Academy of SciencesU.S.A. 844767–4771
    [Google Scholar]
  35. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:324–330
    [Google Scholar]
  36. Toneguzzo F., Glynn S., Levi E., Mjolsness S., Hayday A. 1988; Use of a chemically modified T7 DNA polymerase for manual and automated sequencing of supercoiled DNA. BioTechniques 6:460–469
    [Google Scholar]
  37. Tremaine J. H., Ronald W. P., Mcgauley E. M. 1983; Effect of sodium dextran sulfate on some isometric plant viruses. Phytopathology 73:1241–1246
    [Google Scholar]
  38. Zimmern D. 1988; Evolution of RNA viruses. In RNA Genetics IIRetroviruses, Viroids and RNA Recombination211–240 Domingo E., Holland J. J., Ahlquist P. Boca Raton: CRC Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-11-3033
Loading
/content/journal/jgv/10.1099/0022-1317-70-11-3033
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error