1887

Abstract

SUMMARY

Complementary DNAs of two different satellite RNA isolates from cucumber mosaic virus (CMV), Y from Japan and Ra from France, have been cloned in a transcription vector containing the Pr promoter. When inoculated on plants with CMV RNA (strain KIN), the transcripts of the cloned Y satellite cDNA elicit a bright yellow mosaic on tobacco and a lethal necrosis on tomato. Addition of the transcripts of the Ra satellite cDNA to an inoculum of CMV RNA resulted in symptom attenuation on both tobacco and tomato, in agreement with the characterized symptoms of the natural satellite. Recombinant molecules involving these two satellites have been constructed in order to determine which parts of the Y satellite RNA are involved in symptom induction. The determinant for symptom production on tobacco lies in the region between nucleotides 1 and 219. The domain for necrotic symptoms on tomato resides on the 3′ half of the molecule beyond nucleotide 219.

Keyword(s): CMV satellite RNA , tobacco and tomato
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-10-2765
1989-10-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/10/JV0700102765.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-10-2765&mimeType=html&fmt=ahah

References

  1. Ahlquist P., Janda M. 1984; cDNA cloning and in vitro transcription of the complete brome mosaic virus genome. Molecular and Cellular Biology 4:2876–2882
    [Google Scholar]
  2. Avila-Rincon M. J., Collmer C. W., Kaper J. M. 1986; In vitro translation of cucumoviral satellite I. Purification and nucleotide sequence of cucumber mosaic virus strain S. Virology 152:446–454
    [Google Scholar]
  3. Baulcombe D. C., Saunders G. S., Bevan M. W., Mayo M. A., Harrison B.D. 1986; Expression of biologically active viral satellite RNA from the nuclear genome of transformed plants. Nature London: 321446–449
    [Google Scholar]
  4. Collmer C. W., Kaper J. M. 1986; Infectious RNA transcripts from cloned cDNAs of cucumber mosaic viral satellite. Biochemical and Biophysical Research Communications 135:290–296
    [Google Scholar]
  5. Collmer C. W., Kaper J. M. 1988; Site-directed mutagenesis of potential protein-coding regions in expressible cloned cDNAs of cucumber mosaic viral satellites. Virology 163:293–298
    [Google Scholar]
  6. Feinberg A. P., Vogelstein B. 1984; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137:266–267
    [Google Scholar]
  7. Francki R. I. B. 1985; Plant virus satellites. Annual Review of Microbiology 39:151–174
    [Google Scholar]
  8. Garcia-Arenal F., Zaitlin M., Palukaitis P. 1987; Nucleotide sequence analysis of six satellite RNAs of cucumber mosaic virus: primary sequence and secondary structure alterations do not correlate with differences in pathogenicity. Virology 158:339–347
    [Google Scholar]
  9. Gonsalves D., Provvidenti R., Edwards M. C. 1982; Tomato white leaf: the relation of an apparent satellite RNA and cucumber mosaic virus. Phytopathology 72:1533–1538
    [Google Scholar]
  10. Gordon K. H., Symons R. H. 1983; Satellite RNA of cucumber mosaic virus forms a secondary structure with partial 3′-terminal homology to genomic RNAs. Nucleic Acids Research 11:947–960
    [Google Scholar]
  11. Hamilton W. D. O., Baulcombe D. C. 1989; Infectious RNA produced by in vitro transcription of a full-length tobacco rattle virus RNA-1 cDNA. Journal of General Virology 70:963–968
    [Google Scholar]
  12. Harrison B. D., Mayo M. A., Baulcombe D. C. 1987; Virus resistance in transgenic plants that express cucumber mosaic virus. Nature London: 328779–780
    [Google Scholar]
  13. Hidaka S., Ishikawa K., Takanami Y., Susumu K., Miura K. I. 1984; Complete nucleotide sequence of RNAs from cucumber mosaic virus (strain Y). FEBS Letters 174:38–42
    [Google Scholar]
  14. Hidaka S., Hanada K., Ishikawa K., Miura K. I. 1988; Complete nucleotide sequence of two new satellite RNAs associated with cucumber mosaic virus. Journal of Virology 164:326–333
    [Google Scholar]
  15. Jacquemond M. 1982; Phenomenes d’interference entre les types d’ARN satellite du virus de la mosaïque du concombre. Protection des tomates vis à vis de la necrose lethale. Compte rendu de l’Académie des sciences 294:991–994
    [Google Scholar]
  16. Jacquemond M., Lauquin G. 1988; The cDNA of cucumber mosaic virus-associated satellite RNA has in vivo biological properties. Biochemical and Biophysical Research Communications 151:388–395
    [Google Scholar]
  17. Jacquemond M., Lot H. 1981; L’ARN satellite du virus de la mosaïque du concombre. I. Comparaison de l’aptitude à induire la necrose de la tomate d’ARN satellites isolés de plusieurs souches du virus. Agronomie 1:927–932
    [Google Scholar]
  18. Kaper J. M., Duriat A. S., Tousignant M. E. 1986; The 368-nucleotide satellite of cucumber mosaic virus strain Y from Japan does not cause lethal necrosis in tomato. Journal of General Virology 67:2241–2246
    [Google Scholar]
  19. Kaper J. M., Tousignant M. E., Steen M. T. 1988; Cucumber mosaic virus associated RNAs. XI. Comparison of 14 CARNA 5 variants relate ability to induce tomato necrosis to a conserved nucleotide sequence. Virology 163:284–292
    [Google Scholar]
  20. Kurath G., Palukaitis P. 1989; Satellite RNAs of cucumber mosaic virus: recombinants constructed in vitro reveal independent functional domains for chlorosis and necrosis in tomato. Molecular Plant-Microbe Interactions 2:91–96
    [Google Scholar]
  21. Masuta C., Kuwata S., Takanami Y. 1988; Disease modulation on several plants by cucumber mosaic virus satellite RNA (Y strain). Annals of the Phytopathological Society of Japan 54:332–336
    [Google Scholar]
  22. Mossop D. W., Francki R. I. B. 1979; Comparative studies on two satellite RNAs of cucumber mosaic virus. Virology 95:395–404
    [Google Scholar]
  23. Murphy G., Kavanagh T. 1988; Speeding up the sequencing of double-stranded DNA. Nucleic Acids Research 16:5198
    [Google Scholar]
  24. Palukaitis P. 1988; Pathogenicity regulation by satellite RNAs of cucumber mosaic virus: minor nucleotide sequence changes alter host responses. Molecular Plant-Microbe Interactions 1:175–181
    [Google Scholar]
  25. Scharf S. J., Horn G. T., Erlich H. A. 1986; Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 233:1076–1078
    [Google Scholar]
  26. Sippel A. E. 1973; Purification and characterization of adenosine triphosphate :ribonucleic acid adenyltransfer-ase from Escherichia coli. European Journal of Biochemistry 37:31–40
    [Google Scholar]
  27. Takanami Y. 1981; A striking change on cucumber mosaic virus infected tobacco plants induced by satellite RNA. Virology 109:120–126
    [Google Scholar]
  28. Van Emmelo J., Ameloot P., Fiers W. 1987; Expression in plants of the cloned satellite tobacco virus genome and of derived insertion mutants. Virology 157:480–487
    [Google Scholar]
  29. Waterworth H. E., Kaper J. M., Tousignant M. E. 1979; CARNA 5, the small cucumber mosaic virus-dependent replicating RNA regulates disease expression. Science 204:845–847
    [Google Scholar]
  30. Zuker M., Stiegler P. 1981; Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleic Acids Research 9:133–148
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-10-2765
Loading
/content/journal/jgv/10.1099/0022-1317-70-10-2765
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error