Protection of Cotton Rats against Human Respiratory Syncytial Virus by Vaccination with a Novel Chimeric FG Glycoprotein Free

Abstract

SUMMARY

The cotton rat model of experimental human respiratory syncytial virus (RSV) infection was used to study the efficacy of FG, a novel chimeric glycoprotein which was expressed in insect cells using a baculovirus vector. FG contained the extracellular regions of the F (fusion) and G (attachment) glycoproteins of RSV. Vaccination with FG resulted in induction of neutralizing antibody and was correlated with protection of lung tissue from RSV challenge against both serogroup A and B virus strains. Both crude FG taken from supernatants of insect cells and affinity-purified FG were immunogenic and active against RSV. FG vaccination was effective by three routes of administration, following a single dose, and when administered with different adjuvants.

Keyword(s): glycoprotein protection , human and RSV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-10-2637
1989-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/10/JV0700102637.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-10-2637&mimeType=html&fmt=ahah

References

  1. Elango N., Prince G. A., Murphy B. R., Venkatesan S., Chanock R. M., Moss B. 1986; Resistance to human respiratory syncytial virus -(RSV)infection induced by immunization of cotton rats with a recombinant vaccinia virus expressing the RSV G glycoprotein. Proceedings of the National Academy of SciencesU.S.A 831906–1910
    [Google Scholar]
  2. Kim H., Canchola J., Brandt C., Pyles G., Chanock R., Jenson K., Parrott R. 1969; R.S. virus disease in infants despite prior administration of antigenic inactivated vaccine. American Journal of Epidemiology 89:422–434
    [Google Scholar]
  3. Kim H. W., Arrobio J. O., Pyles G., Brandt C. D., Camargo E., Chanock R. M., Parrott R. H. 1971; Clinical and immunological response of infants and children to administration of low-temperature adapted respiratory syncytial virus. Pediatrics 58:745–755
    [Google Scholar]
  4. Moore R. M., Walters R. R. 1984; Protein separations on reversed-phase high-performance liquid chromatography minicolumns. Journal of Chromatography 317:119–128
    [Google Scholar]
  5. Olmsted R. A., Elango N., Prince G. A., Murphy B. R., Johnson P. R., Moss B., Chanock R. M., Collins P. L. 1986; Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the F and G glycoproteins to host immunity. Proceedings of the National Academy of SciencesU.S.A. 837462–7466
    [Google Scholar]
  6. Parrott R. H., Kim H. W., Arrobio I. O., Hodes D. S., Murphy B. R., Brandt C. D., Camargo E., Chanock R. M. 1973; Epidemiology of respiratory syncytial virus infection in Washington, DC. II. Infection and disease with respect to age, immunological status, race, and sex. American Journal of Epidemiology 98:289–300
    [Google Scholar]
  7. Petrovskis E. A., Timmins J. G., Armentrout M. A., Marchioli C. C., Yancey R. J., Post L. E. 1986; DNA sequence of the gene for pseudorabies virus gp50, a glycoprotein without N-linked glycosylation. Journal of Virology 59:216–223
    [Google Scholar]
  8. Stott E. J., Ball L. A., Young K., Furze J., Wertz G. W. 1986; Human respiratory syncytial virus glycoprotein G expressed from a recombinant vaccinia virus vector protects mice against live virus challenge. Journal of Virology 60:607–613
    [Google Scholar]
  9. Walsh E. E., Hall C. B., Briselli M., Brandriss M. W., Schlesinger J. J. 1987; Immunization with glycoprotein subunits of respiratory syncytial virus to protect cotton rats against viral infection. Journal of Infectious Diseases 155:1198–1204
    [Google Scholar]
  10. Walters R. R. 1985 In Affinity Chromatography, A Practical Approach25–30 Dean P. D. G., Johnson W. S., Middle F. A. Oxford: IRL Press;
    [Google Scholar]
  11. Wathen M. W., Brideau R. J., Thomsen D. R. 1989a; Immunization of cotton rats with the human respiratory syncytial virus F glycoprotein produced using a baculovirus vector. Journal of Infectious Diseases 159:255–263
    [Google Scholar]
  12. Wathen M. W., Brideau R. J., Thomsen D. R., Murphy B. R. 1989b; Characterization of a novel human respiratory syncytial virus chimeric FG glycoprotein expressed using a baculovirus vector. Journal of General Virology 70:2625–2635
    [Google Scholar]
  13. Wertz G. W., Stott E. J., Young K. Y., Anderson K., Ball L. A. 1987; Expression of the fusion protein of human respiratory syncytial mice. Journal of Virology 61:193–301
    [Google Scholar]
  14. Wright P. F., Belshe R. B., Kim H. W., van Voris L. P., Chanock R. M. 1982; Administration of a highly attenuated, live respiratory syncytial virus vaccine to adults and children. Infection and Immunity 37:397–400
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-10-2637
Loading
/content/journal/jgv/10.1099/0022-1317-70-10-2637
Loading

Data & Media loading...

Most cited Most Cited RSS feed