RT Journal Article SR Electronic(1) A1 Wathen, M. W. A1 Brideau, R. J. A1 Thomsen, D. R. A1 Murphy, B. R.YR 1989 T1 Characterization of a Novel Human Respiratory Syncytial Virus Chimeric FG Glycoprotein Expressed Using a Baculovirus Vector JF Journal of General Virology, VO 70 IS 10 SP 2625 OP 2635 DO https://doi.org/10.1099/0022-1317-70-10-2625 PB Microbiology Society, SN 1465-2099, AB SUMMARY Human respiratory syncytial virus (RSV) codes for two glycoproteins (F and G) which have been shown to be the major targets for the host antibody response. We have expressed a novel chimeric glycoprotein (FG) in insect cells using a baculovirus vector. The chimeric glycoprotein contains the signal and extracellular regions of the RSV F glycoprotein linked to the extracellular region of the RSV G glycoprotein. Beginning at the amino terminus, the chimeric glycoprotein consists of amino acids 1 to 489 from RSV F followed by amino acids 97 to 279 from RSV G. The chimeric FG glycoprotein did not contain an anchor region and was efficiently secreted into the medium of recombinant baculovirus-infected insect cells. The FG glycoprotein ranged in size from 69K to 91K and was heterogeneous with respect to isoelectric point. The cleavage site present on the F glycoprotein was recognized on the chimeric FG, and the glycoprotein appeared to be antigenically similar to the native RSV F and G glycoproteins., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-70-10-2625