The reassortment of genome segments during mixed infection of Vero cells with bluetongue virus (BTV) serotypes 10 and 17 was investigated, using non-selective conditions for analysis of the progeny of mixed infections. Reassortment was found to be an early event in the BTV replication cycle, indicating that progeny BTV genomes undergo a single round of reassortment. Non-random segregation of individual genome segments was observed in crosses at equal multiplicity of infection, and was confirmed in crosses performed at unequal multiplicity. Asynchronous infections showed that superinfection exclusion resulted in the failure of the superinfecting virus to contribute genome segments to reassortants if the second virus followed the first by more than 4 h. The significance of these results for the evolution and epidemiology of BTV is discussed.
BarberT. L.1979; Temporal appearance, geographic distribution and species of origin of bluetongue virus serotypes in the United States. American Journal of Veterinary Research 40:1654–1656
BeatyB. J.,
TrentD. W.,
RoehrigJ. T.1988; Virus variation and evolution: mechanisms and epidemiological significance. In Arboviruses: Epidemiology and Ecology159–86MonathT. P.
Boca Raton: CRC Press;
CowleyJ. A.,
GormanB. M.1987; Genetic reassortants for identification of the genome segment coding for the bluetongue virus hemagglutinin. Journal of Virology 61:2304–2306
DelwartE. L.,
PanganibanA. T.1989; Role of reticuloendotheliosis virus envelope glycoprotein in superinfection interference. Journal of Virology 63:273–280
DittmarD.,
CastroA.,
HainesH.1982; Demonstration of interference between dengue virus types in cultured mosquito cells using monoclonal antibody probes. Journal of Virology 59:272–282
GibbsE. P. J.,
GreinerE. C.,
TaylorW. P.,
BarberT. L.,
HouseJ. A.,
PearsonJ. E.1983; Isolation of bluetongue virus serotype 2 from cattle in Florida: a serotype of bluetongue virus hitherto unrecognized in the western hemisphere. American Journal of Veterinary Research 44:2226–2228
GrubmanM. J.,
AppletonJ. A.,
LetchworthG. J.1983; Identification of bluetongue virus type 17 genome segments coding for polypeptides associated with virus neutralization and intergroup reactivity. Virology 131:355–366
MertensP. P. C,
BrownF.,
sangarD. V.1984; Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology 135:207–217
OberstR. D.,
SquireK. R. E.,
StottJ. L.,
ChuangR. Y.,
OsburnB. I.1985; The coexistence of multiple bluetongue virus electropherotypes in individual cattle during natural infection. Journal of General Virology 66:1901–1909
RamigR. F.1983; Factors that affect genetic interaction during mixed infection with temperature-sensitivemutants of simian rotavirus SA11. Virology 127:91–99
SamalS. K.,
McconnellS.,
RamigR. F.1985; Growth characteristics of virulent and attenuated strains of bluetongue virus serotypes 10, 11, 13, and 17. In Bluetongue and Related Orbiviruses407–412BarberT. L.,
JochimM. M.
New York: Alan R. Liss;
SamalS. K.,
LivingstonC. W.,
McconnellS.,
RamigR. F.1987a; Analysis of mixed infection of sheep with bluetongue virus serotypes 10 and 17: evidence for reassortment in the vertebrate host. Journal of Virology 61:1086–1091
SamalS. K.,
El husseinA.,
HolbrookF. R.,
BeatyB. J.,
RamigR. F.1987b; Mixed infection of Culicoides variipennis with bluetongue virus serotypes 10 and 17: evidence for high frequency reassortment in the vector. Journal of General Virology 68:2319–2329
ShiphamS. O.,
De La ReyM.1976; The isolation and preliminary genetic classification of temperature-sensitive mutants of bluetongue virus. Onderstepoort Journal of Veterinary Research 43:189–192
ShiphamS. O.,
De La ReyM.1979; Temperature-sensitive mutants of bluetongue virus: genetic and physiological characterization. Onderstepoort Journal of Veterinary Research 46:87–94
StottJ. L.,
OsburnB. I.,
BarberT. L.1982; Recovery of dual serotypes of bluetongue virus from infected sheep and cattle. Veterinary Microbiology 7:197–207
StottJ. L.,
OberstR. D.,
ChannelM. B.,
OsburnB. I.1987; Genome reassortment between two serotypes of bluetongue virus in a natural host. Journal of Virology 61:2670–2674
SundinD. R.,
BeatyB. J.1988; Interference to oral superinfection of Aedes triseriatus infected with La Crossevirus. American Journal of Tropical Medicine and Hygiene 83:428–432