RT Journal Article SR Electronic(1) A1 Nakajima, Kazuhiro A1 Shibayama, Takeshi A1 Yokota, Miya A1 Ikuta, Kazuyoshi A1 Kato, Shiro A1 Hirai, KanjiYR 1989 T1 Identification of Virus-specific Polypeptides by Monoclonal Antibodies against Serotype 2 Marek’s Disease Virus JF Journal of General Virology, VO 70 IS 10 SP 2563 OP 2571 DO https://doi.org/10.1099/0022-1317-70-10-2563 PB Microbiology Society, SN 1465-2099, AB SUMMARY A total of 41 antibody-secreting hybridoma cells against the HPRS24 strain of Marek’s disease virus (MDV) type 2 (MDV2) have been isolated. Of these monoclonal antibodies (MAbs), 24 were found by immunofiuorescence tests to react specifically with MDV2-infected cells, but not MDV type 1 (MDV1)- or herpesvirus of turkeys (HVT)-infected cells, while eight reacted with MDV1- or MDV2-infected cells and nine with MDV1-, MDV2- or HVT-infected cells. By using these MAbs, seven classes of MDV type-specific or cross-reactive polypeptides were characterized by immuno-precipitation followed by SDS‒PAGE. Among them, a 28K/32K glycoprotein differed from the previously identified gA and gB. The 28K/32K glycoprotein was found on the surface of MDV2-infected cells and in the cytoplasm by an immunofluorescence test with MAbs. In addition, a cross-reactive polypeptide of 25K/29K was also detected in MDV1-infected cells with MAbs reactive with the 28K/32K glycoprotein of MDV2., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-70-10-2563