1887

Abstract

SUMMARY

A total of 41 antibody-secreting hybridoma cells against the HPRS24 strain of Marek’s disease virus (MDV) type 2 (MDV2) have been isolated. Of these monoclonal antibodies (MAbs), 24 were found by immunofiuorescence tests to react specifically with MDV2-infected cells, but not MDV type 1 (MDV1)- or herpesvirus of turkeys (HVT)-infected cells, while eight reacted with MDV1- or MDV2-infected cells and nine with MDV1-, MDV2- or HVT-infected cells. By using these MAbs, seven classes of MDV type-specific or cross-reactive polypeptides were characterized by immuno-precipitation followed by SDS‒PAGE. Among them, a 28K/32K glycoprotein differed from the previously identified gA and gB. The 28K/32K glycoprotein was found on the surface of MDV2-infected cells and in the cytoplasm by an immunofluorescence test with MAbs. In addition, a cross-reactive polypeptide of 25K/29K was also detected in MDV1-infected cells with MAbs reactive with the 28K/32K glycoprotein of MDV2.

Keyword(s): MAbs , MDV2 and polypeptides
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-10-2563
1989-10-01
2024-12-09
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/10/JV0700102563.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-10-2563&mimeType=html&fmt=ahah

References

  1. Biggs P. M., Milne B. S. 1972; Biological properties of a number of Marek’s disease virus isolates. In Oncogenesis and Herpesvirus I88–94 Biggs P. M., De-The G., Payne L. N. Lyon: International Agency for Research on Cancer;
    [Google Scholar]
  2. Buckmaster A. E., Scott S. D., Sanderson M. J., Boursnell M. E. G., Ross N. L. J., Binns M. M. 1988; Gene sequence and mapping data from Marek’s disease virus and herpesvirus of turkeys: implications for herpesvirus classification. Journal of General Virology 69:2033–2042
    [Google Scholar]
  3. Calnek B. W., Schat K. A., Peckham M. C., Fabricant J. 1983; Field trials with a bivalent vaccine (HVT and SB-1) against Marek’s disease. Avian Diseases 27:844–849
    [Google Scholar]
  4. Eidson C. S., Page R. K., Kleven S. H. 1978; Effectiveness of cell-free or cell-associated turkey herpesvirus vaccine against Marek’s disease in chickens as influenced by maternal antibody, vaccine dose, and time of exposure to Marek’s disease virus. Avian Diseases 22:583–597
    [Google Scholar]
  5. Eidson C. S., Ellis M. N., Kleven S. H. 1981; Reduced vaccinal protection of turkey herpesvirus against field strains of Marek’s disease herpesvirus. Poultry Science 60:317–322
    [Google Scholar]
  6. Hirai K., Nakajima K., Ikuta K., Kirisawa R., Mikami T., Kato S. 1986; Similarities and dissimilarities in the structure and expression of viral genomes of various virus strains immunologically related to Marek’s disease virus. Archives of Virology 89:113–130
    [Google Scholar]
  7. Hirose H., Matsuda H., Murata M., Sekiya Y. 1987; Monoclonal antibodies specific for Marek’s disease virus and herpesvirus of turkeys. Japanese Journal of Veterinary Science 48:1263–1266
    [Google Scholar]
  8. Ikuta K., Nishi Y., Kato S., Hirai K. 1981; Immunoprecipitation of Marek’s disease virus-specific polypeptides with chicken antibodies purified by affinity chromatography. Virology 114:277–281
    [Google Scholar]
  9. Ikuta K., Honma H., Maotani K., Ueda S., Kato S., Hirai K. 1982; Monoclonal antibodies specific to and cross-reactive with Marek’s disease virus and herpesvirus of turkeys. Biken Journal 25:171–175
    [Google Scholar]
  10. Ikuta K., Ueda S., Kato S., Hirai K. 1983; Monoclonal antibodies reactive with the surface and secreted glycoproteins of Marek’s disease virus and herpesvirus of turkeys. Journal of General Virology 64:2597–2610
    [Google Scholar]
  11. Ikuta K., Ueda S., Kato S., Hirai K. 1984a; Identification with monoclonal antibodies of glycoproteins of Marek’s disease virus and herpesvirus of turkeys related to virus neutralization. Journal of Virology 49:1014–1017
    [Google Scholar]
  12. Ikuta K., Nakajima K., Ueda S., Kato S., Hirai K. 1984b; Studies on the serological cross-reaction between Marek’s disease virus and herpesvirus of turkeys using monoclonal antibodies to major virus-specific polypeptides. Archives of Virology 81:337–343
    [Google Scholar]
  13. Ikuta K., Nakajima K., Naito M., An S. H., Ueda S., Kato S., Hirai K. 1985; Identification of Marek’s disease virus-specific antigens in Marek’s disease lymphoblastoid cell lines using monoclonal antibody against virus specific phosphorylated polypeptides. International Journal of Cancer 35:257–264
    [Google Scholar]
  14. Kato S., Ono K., Naito M., Doi T., Iwa N., Mori Y., Onoda T. 1970; Isolation of herpes-type virus from chickens with Marek’s disease using duck embryo fibroblast cultures. Biken Journal 13:193–203
    [Google Scholar]
  15. Lee L. F., Liu X., Witter R. L. 1983; Monoclonal antibodies with specificity for three different serotypes of Marek’s disease virus in chickens. Journal of Immunology 130:1003–1006
    [Google Scholar]
  16. Naito M., Nakajima K., Iwa N., Ono K., Yoshida I., Konobe T., Ikuta K., Ueda S., Kato S., Hirai K. 1986; Demonstration of a Marek’s disease virus-specific antigen in tumour lesions of chickens with Marek’s disease using monoclonal antibody against a virus phosphorylated protein. Avian Pathology 15:503–510
    [Google Scholar]
  17. Nakajima K., Ikuta K., Naito M., Ueda S., Kato S., Hirai K. 1986; Identification with monoclonal antibodies of virus-specific DNA-binding proteins in the nuclei of cells infected with three serotypes of Marek’s disease virus related viruses. Journal of Virology 59:154–158
    [Google Scholar]
  18. Nakajima K., Ikuta K., Naito M., Ueda S., Kato S., Hirai K. 1987; Analysis of Marek’s disease virus serotype 1-specific phosphorylated polypeptides in virus-infected cells and Marek’s disease lymphoblastoid cells. Journal of General Virology 68:1379–1389
    [Google Scholar]
  19. Ono K., Doi T., Ishikawa T., Iwa N., Naito M., Kato S., Koyama K., Konobe T., Takaku K. 1974; Studies on herpesvirus of turkeys. I. Isolation of herpesvirus from turkeys and its virological characteristics. Japanese Journal of Veterinary Science 36:407–420
    [Google Scholar]
  20. Ono K., Takashima M., Ishikawa T., Hayashi M., Yoshida I., Konobe T., Ikuta K., Nakajima K., Ueda S., Kato S., Hirai K. 1985; Partial protection against Marek’s disease in chickens immunized with glycoprotein gB purified from turkey-herpesvirus-infected cells by affinity chromatography coupled with monoclonal antibodies. Avian Diseases 29:533–539
    [Google Scholar]
  21. Rispens B. H., Von Vloten H., Mastenbroek N., Mass H. J. l., Schat K. A. 1972; Control of Marek’s disease in the Netherlands. II. Field trials on vaccination with an avirulent strain (CVI988) of Marek’s disease virus. Avian Diseases 16:108–125
    [Google Scholar]
  22. Schat K. A., Calnek B. W. 1978; Characterization of an apparently nononcogenic Marek’s disease virus. Journal of the National Cancer Institute 60:1075–1081
    [Google Scholar]
  23. Sevoian M., Chamberlain D. M., Counter F. 1962; Avian lymphomatosis. Experimental reproduction of the neural and visceral forms. Veterinary Medicine 57:500–501
    [Google Scholar]
  24. Silva R. F., Lee L. F. 1984; Monoclonal antibody-mediated immunoprecipitation of proteins from cells infected with Marek’s disease virus or turkey herpesvirus. Virology 136:307–320
    [Google Scholar]
  25. Spencer J. L., Grunder A. A., Robertson A., Speckmann G. W. 1972; Attenuated Marek’s disease herpesvirus: protection conferred on strains of chickens varying in genetic resistance. Avian Diseases 16:94–107
    [Google Scholar]
  26. Van zaane D., Brinkhof J. M. A., Westenbrink F., Gielkens A. L. J. 1982; Molecular-biological characterization of Marek’s disease virus. I. Identification of virus-specific polypeptides in infected cells. Virology 121:116–132
    [Google Scholar]
  27. Von bülow V., Biggs P. M. 1975a; Differentiation between strains of Marek’s disease virus and turkey herpesvirus by immunofluorescence assays. Avian Pathology 4:133–146
    [Google Scholar]
  28. Von bülow V., Biggs P. M. 1975b; Precipitating antigens associated with Marek’s disease virus and a herpesvirus of turkeys. Avian Pathology 4:147–162
    [Google Scholar]
  29. Witter R. L. 1982; Protection by attenuated and polyvalent vaccines against highly virulent strains of Marek’s disease virus. Avian Pathology 11:49–62
    [Google Scholar]
  30. Witter R. L. 1983; Characteristics of Marek’s disease viruses isolated from vaccinated commercial chicken flocks: association of viral pathotype with lymphoma frequency. Avian Diseases 27:113–132
    [Google Scholar]
  31. Witter R. L., Nazerian K., Purchase H. G., Burgoyne G. H. 1970; Isolation from turkeys of a cell-associated herpesvirus antigenically related to Marek’s disease. American Journal of Veterinary Research 31:525–538
    [Google Scholar]
  32. Witter R. L., Sharma J. M., Fadly A. M. 1980; Pathogenicity of variant Marek’s disease virus isolants in vaccinated and unvaccinated chickens. Avian Diseases 24:210–232
    [Google Scholar]
  33. Witter R. L., Sharma J. M., Lee L. F. 1984; Field trials to test the efficacy of polyvalent Marek’s disease vaccines in broilers. Avian Diseases 28:44–60
    [Google Scholar]
  34. Witter R. L., Silva R. F., Lee L. F. 1987; New serotype 2 and attenuated serotype 1 Marek’s disease vaccine viruses: selected biological and molecular characteristics. Avian Diseases 31:829–840
    [Google Scholar]
  35. Wyn-Jones A. P., Kaaden O.-R. 1979; Induction of virus-neutralizing antibody by glycoproteins isolated from chicken cells infected with herpesvirus of turkeys. Infection and Immunity 25:54–59
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-70-10-2563
Loading
/content/journal/jgv/10.1099/0022-1317-70-10-2563
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error