1887

Abstract

SUMMARY

We have determined the nucleotide sequence of an almost full-length clone of porcine parvovirus (PPV). The sequence is 4973 nucleotides (nt) long. The 3′ end of virion DNA shows a Y-shaped configuration homologous to rodent parvoviruses. The 5′ end of virion DNA shows a repetition of 127 nt at the carboxy terminus of the capsid proteins. The overall organization of the PPV genome is similar to those of other autonomous parvoviruses. There are two large open reading frames (ORFs) that almost entirely cover the genome, both located in the same frame of the complementary strand. The left ORF encodes the non-structural protein NS1 and the right ORF encodes the capsid proteins (VP1, VP2 and VP3). Promoter analysis, location of splicing sites and putative amino acid sequences for the viral proteins show a high homology of PPV with feline panleukopenia virus and canine parvoviruses (FPV and CPV) and rodent parvovirus. Therefore we conclude that PPV is related to the Kilham rat virus (KRV) group of autonomous parvoviruses formed by KRV, minute virus of mice, Lu III, H-1, FPV and CPV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-10-2541
1989-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/10/JV0700102541.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-10-2541&mimeType=html&fmt=ahah

References

  1. Armentrout R., Bates R., Berns K., Carter B., Chow M., Dressler D., Fife K., Hauswirth W., Hayward G., Lavelle G., Rhode S., Straus S., Tattersall P., Ward D. 1978; A standardized nomenclature for restriction endonuclease fragments. In Replication of Mammalian Parvoviruses523–526 Ward D., Tattersall P. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  2. Astell C. R., Gardiner E. M., Tattersall P. 1986; DNA sequence of the lymphotropic variant of minute virus of mice, MVM (i), and its comparison with the DNA sequence of the fibrotopic prototype strain. Journal of Virology 57:656–659
    [Google Scholar]
  3. Bensinhon M., Gabarro-arpa J., Ehrlich R., Reiss C. 1983; Physical characteristics in eucaryotic promoters. Nucleic Acids Research 11:4521–4540
    [Google Scholar]
  4. Berget S. M. 1984; Are U4 small nuclear riboproteins involved in polyadenylation?. Nature, London 309:179–182
    [Google Scholar]
  5. Birnstiel M. L., Busslinger M., Strub K. 1985; Transcription termination and 3′ processing: the end is in site. Cell 41:349–359
    [Google Scholar]
  6. Carlson J. R., Rushlow K., Maxwell I., Maxwell F., Winston S., Hahn W. 1985; Cloning and sequence of DNA encoding structural proteins of the autonomous parvovirus feline panleukopenia virus. Journal of Virology 55:574–582
    [Google Scholar]
  7. Chen K. C., Shull B. C., Moses E. A., Lederman M., Stout F. R., Bates R. C. 1986; Complete nucleotide sequence and genome organization of bovine parvovirus. Journal of Virology 60:1085–1097
    [Google Scholar]
  8. Choi C. S., Molitor T. W., Joo H. S. 1987; Inhibition of porcine parvovirus replication by empty virus particles. Archives of Virology 96:75–87
    [Google Scholar]
  9. Cotmore S. F., Tattersall P. 1987; The autonomously replicating parvoviruses of vertebrates. Advances in Virus Research 33:91–174
    [Google Scholar]
  10. Halling S. M., Smith S. 1985; Expression in Escherichia coli of multiple products from a chimaeric gene fusion: evidence for the presence of procaryotic translational control regions within eucaryotic genes. Bio/Technology 3:715–720
    [Google Scholar]
  11. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:557–580
    [Google Scholar]
  12. Hauswirth W. W. 1984; Autonomous parvovirus DNA structure and replication. In The Parvoviruses129–150 Berns K. I. New York: Plenum Press;
    [Google Scholar]
  13. Jongeneel C. V., Sahli R., Mcmaster G. K., Hirt B. 1986; A precise map of splice junctions in the mRNAs of minute virus of mice, an autonomous parvovirus. Journal of Virology 59:564–573
    [Google Scholar]
  14. Joo H. S., Johnson R. H. 1976; Porcine parvovirus: a review. Veterinary Bulletin 46:653–660
    [Google Scholar]
  15. Kozak M. 1983; Comparison of initiation of protein synthesis in procaryotes, eucaryotes and organelles. Microbiological Reviews 47:1–45
    [Google Scholar]
  16. Langridge J., Langridge P., Bergquist P. L. 1980; Extraction of nucleic acids from agarose gel. Analytical Biochemistry 103:264–271
    [Google Scholar]
  17. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Mengeling W. L. 1978; Prevalence of porcine parvovirus induced reproductive failure: an abortion study. Journal of the American Veterinary Medical Association 111:1291–1294
    [Google Scholar]
  19. Mengeling W. L., Cutlip R. C. 1976; Reproductive disease experimentally induced by exposing pregnant gilts to porcine parvovirus. American Journal of Veterinary Research 37:1393–1399
    [Google Scholar]
  20. Mengeling W. L., Paul P. S., Bunn T. O., Ridpath J. F. 1986; Antigenic relationships among autonomous parvoviruses. Journal of General Virology 67:2839–2844
    [Google Scholar]
  21. Messing J. 1983; New M13 vectors for cloning. Methods in Enzymology 101:20–76
    [Google Scholar]
  22. Molitor T. W., Joo H. S., Oollett M. S. 1983; Porcine parvovirus: virus purification and structural and antigenic properties of virion polypeptides. Journal of Virology 45:842–854
    [Google Scholar]
  23. Molitor T. W., Joo H. S., Collett M. S. 1984; Porcine parvovirus DNA: characterization of the genomic and replicative form DNA of two virus isolates. Virology 137:241–254
    [Google Scholar]
  24. Molitor T. W., Joo H. S., Collett M. S. 1985; Identification and characterization of a porcine parvovirus nonstructural polypeptide. Journal of Virology 55:554–559
    [Google Scholar]
  25. Mount S. M. 1982; A catalogue of splice junction sequences. Nucleic Acids Research 10:459–472
    [Google Scholar]
  26. Paradiso P. R. 1984; Identification of multiple forms of the noncapsid parvovirus protein NCVP1in H-1 parvovirus-infected cells. Journal of Virology 52:82–87
    [Google Scholar]
  27. Pirtle E. C. 1984; Titration of two porcine respiratory viruses in mammalian cell culture by direct fluorescent antibody staining. American Journal of Veterinary Research 34:249–250
    [Google Scholar]
  28. Queen C., Korn L. S. 1984; A comprehensive sequence analysis program for the IBM PC. Nucleic Acids Research 12:581–599
    [Google Scholar]
  29. Reed A. P., Jones E. V., Miller T. J. 1988; Nucleotide sequence and genome organization of canine parvovirus. Journal of Virology 62:266–276
    [Google Scholar]
  30. Rhode S. L. 1985; Nucleotide sequence of the coat protein gene of canine parvovirus. Journal of Virology 54:630–633
    [Google Scholar]
  31. Rhode S. L., Paradiso P. K. 1983; Parvovirus genome: nucleotide sequence of H-1 and mapping of its genes by hybrid-arrested translation. Journal of Virology 45:173–184
    [Google Scholar]
  32. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of SciencesU.S.A. 74:5463–5467
    [Google Scholar]
  33. Shade R. O., Blundell M. C., Cotmore S. F., Tattersall P., Astell R. C. 1986; Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis. Journal of Virology 58:921–936
    [Google Scholar]
  34. Srivastava A., Lubsky E. W., Berns K. I. 1983; Nucleotide sequence and organization of the adeno-associated virus 2 genome. Journal of Virology 45:555–564
    [Google Scholar]
  35. Stephens R. M. 1985; A sequencers’ sequence analysis package for the IBM PC. Gene Analysis Techniques 2:67–75
    [Google Scholar]
  36. Wickens M., Stephenson P. 1984; Role of the conserved AAUA AA sequence: four AAUAAA point mutations prevent messenger RNA 3′ end formation. Science 226:1045
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-10-2541
Loading
/content/journal/jgv/10.1099/0022-1317-70-10-2541
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error