1887

Abstract

SUMMARY

Human sera containing respiratory syncytial (RS) virus-specific antibodies enhance RS virus infection of the U937 macrophage cell line. There was an increase in the number of cells expressing virus antigen when U937 cells were infected with RS virus in the presence of human serum compared to cells infected in the absence of human serum. Human sera enhanced virus yield, as measured by the cell-released infectious virus, by an average of 50-fold compared to virus infection in the absence of human serum. The comparison of the enhancing activities of paired acute and convalescent human sera showed that the titre of enhancing antibody increased in parallel with the titre of RS virus-specific antibody measured by complement fixation and virus neutralization. An RS virus-specific neutralizing monoclonal antibody directed to the virus F protein enhanced virus infection of U937 cells. A non-neutralizing monoclonal antibody directed to the virus nucleoprotein did not enhance virus infection. The possible role of enhancing antibodies is discussed.

Keyword(s): MAbs , macrophage and RS virus
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1989-01-01
2022-01-22
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References

  1. Brandt W. E., Gentry M. K., Russell P. K. 1982; Infection enhancement of dengue type 2 virus in the U-937 human monocyte cell line by antibodies to flavivirus cross-reactive determinants. Infection and Immunity 36:1036–1041
    [Google Scholar]
  2. Burstin S. J., Brandriss M. W., Schlesinger J. I. 1983; Infection of a macrophage-like cell line, P388D1, with reovirus; effects of immune ascitic fluids and monoclonal antibodies on neutralization and on enhancement of viral growth. Journal of Immunology 130:2915–2919
    [Google Scholar]
  3. Cardosa M. J., Gordon S., Hirsch S., Springer T. A., Porterfield J. S. 1986; Interaction of West Nile virus with primary murine macrophages: role of cell activation and receptors for antibody and complement. Journal of Virology 57:952–959
    [Google Scholar]
  4. Chin J., Magoffin R. L., Shearer L. A., Schieble J. H., Lennette E. H. 1969; Field evaluation of a respiratory syncytial virus vaccine and a trivalent parainfluenza virus vaccine in a pediatric population. American Journal of Epidemiology 89:449–163
    [Google Scholar]
  5. Dumorat F., Roberts N. J., Walsh E. E., Dagan R. 1985; Respiratory syncytial virus infection of human mononuclear leukocytes in vitro and in vivo. Journal of Infectious Diseases 152:895–902
    [Google Scholar]
  6. Gimenez H. B., Cash P., Melvin W. T. 1984; Monoclonal antibodies to human respiratory syncytial virus and their use in comparison of different virus isolates. Journal of General Virology 65:963–971
    [Google Scholar]
  7. Gimenez H. B., Hardman N., Keir H. M., Cash P. 1986; Antigenic variation between human respiratory syncytial virus isolates. Journal of General Virology 67:863–870
    [Google Scholar]
  8. Gimenez H. B., Keir H. M., Cash P. 1987; Immunoblot analysis of the human antibody response to respiratory syncytial virus infection. Journal of General Virology 68:1267–1275
    [Google Scholar]
  9. Glezen W. P., Paredes A., Allison J. E., Taber L. H., Frank A. L. 1981; Risk of respiratory syncytial virus infection for infants from low-income families in relationship to age, sex, ethnic group and maternal antibody level. Journal of Pediatrics 98:708–715
    [Google Scholar]
  10. Gollins S. W., Porterfield J. S. 1984; Flavivirus infection enhancement in macrophages: radioactive and biological studies on the effect of antibody on viral fate. Journal of General Virology 65:1261–1272
    [Google Scholar]
  11. Halstead S. B. 1982; Immune enhancement of viral infection. Progress in Allergy 31:301–364
    [Google Scholar]
  12. Halstead S. B., Larsen K., Kliks S., Peiris S. M., Cardosa J., Porterfield J. S. 1983; Comparison of P388D1 mouse macrophage cell line and human monocytes for assay of dengue-2 infection-enhancing antibodies. American Journal of Tropical Medicine and Hygiene 32:157–163
    [Google Scholar]
  13. Halstead S. B., Venkateshan C. N., Gentry M. K., Larsen L. K. 1984; Heterogeneity of infection enhancement of dengue 2 strains by monoclonal antibodies. Journal of Immunology 132:1529–1532
    [Google Scholar]
  14. Kim H. W., Canchola J. G., Brandt C. D., Pyles G., Chanock R. M., Jensen K., Parrott R. H. 1969; Respiratory syncytial virus disease in infants despite administration of antigenic inactivated vaccine. American Journal of Epidemiology 89:422–434
    [Google Scholar]
  15. Krilov L. R., Hendry R. M., Godfrey E., Mclntosh K. 1987; Respiratory virus infection of peripheral blood monocytes: correlation with ageing of cells and interferon production in vitro. Journal of General Virology 68:1749–1753
    [Google Scholar]
  16. Murphy B. R., Prince G. A., Walsh E. E., Kim H. W., Parrott R. H., Hemming V. G., Rodgriguez W. J., Chanock R. M. 1986; Dissociation between serum neutralizing and glycoprotein antibody responses of infants and children who received inactivated respiratory syncytial virus vaccine. Journal of Clinical Microbiology 24:197–202
    [Google Scholar]
  17. Peiris J. S. M., Porterfield J. S. 1981; Antibody-dependent enhancement of plaque formation on cell lines of macrophage origin - a sensitive assay for antiviral antibody. Journal of General Virology 57:119–125
    [Google Scholar]
  18. Peiris J. S. M., Gordon S., Unkeless J. C., Porterfield J. S. 1981; Monoclonal anti-Fc receptor IgG blocks antibody enhancement of viral replication in macrophages. Nature London: 289189–191
    [Google Scholar]
  19. Porterfield J. S. 1982; Immunological enhancement and the pathogenesis of dengue haemorrhagic fever. Journal of Hygiene 89:355–364
    [Google Scholar]
  20. Pringle C. R., Cross A. 1978; Neutralisation of respiratory syncytial virus by cat serum. Nature London: 276501–502
    [Google Scholar]
  21. Schlesinger J. J., Brandriss M. W. 1981a; Antibody-mediated infection of macrophages and macrophage-like cell lines with 17D yellow fever virus. Journal of Medical Virology 8:103–117
    [Google Scholar]
  22. Schlesinger J. J., Brandriss M. W. 1981b; Growth of 17D yellow fever virus in a macrophage-like cell line, U-937: role of Fc and viral receptors in antibody-mediated infection. Journal of Immunology 127:659–665
    [Google Scholar]
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