1887

Abstract

SUMMARY

Polyclonal and monoclonal antibodies have been raised against a fusion protein containing -galactosidase and part of the major capsid protein L1 of the human papillomavirus (HPV) type 16. The polyclonal antibodies cross-reacted with the L1 protein of several HPV types including HPV-1, -2, -6 and -11 when reacted with virus-infected tissue sections, and with HPV-6 and -18 L1 fusion proteins on Western blotting. Monoclonal antibodies against the L1 fusion protein of HPV-16 reacted only with HPV-16 L1 fusion proteins on Western blots and with HPV-16-containing biopsy sections as assessed by DNA–DNA hybridization. These antibodies did not detect HPV-6 L1 protein after Western blotting or in HPV-6-infected tissue sections, although one did react with an HPV-18 fusion protein after Western blotting. The monoclonal antibodies were able to detect HPV-16 antigens in routine formaldehyde-fixed, wax-embedded sections of cervical intraepithelial neoplasia sections. HPV-16 L1 proteins were seen in one-third of biopsies that were positive using the polyclonal cross-reacting antisera. Polyclonal antibodies to fusion proteins containing part of the minor capsid protein L2 of HPV-6 or -16 appeared to be more type-specific as no cross-reactivity was seen when these antibodies were reacted with HPV-1- and -2-infected tissue sections.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-1-69
1989-01-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/1/JV0700010069.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-1-69&mimeType=html&fmt=ahah

References

  1. Baker C. C. 1987; Sequence analysis of papillomavirus genomes. In The Papovaviridae 2321–385 Salzman N. P., Howley P. M. New York: Plenum Press;
    [Google Scholar]
  2. Banks L., Matlashewski C., Pim D., Churcher M., Roberts C., Crawford L. 1987; Expression of human papillomavirus type 6 and type 16 capsid proteins in bacteria and their antigenic characterization. Journal of General Virology 68:3081–3089
    [Google Scholar]
  3. Boshart M., Gissmann L., Ikenberg H., Kleinberg A., Scheurlen W., Zur Hausen H. 1984; A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO Journal 3:1151–1157
    [Google Scholar]
  4. Campion M. J., McCance D. J., Cuzick I., Singer A. 1986; The progressive potential of mild cervical atypia: a rospective cytological, colposcopic and virological study. Lancet ii:237–240
    [Google Scholar]
  5. Collins J., Jenkins J. D., McCance D. J. 1988; HPV DNA sequences in premalignant and malignant disease of the cervix: a retrospective study. Journal of Clinical Pathology 41:289–295
    [Google Scholar]
  6. Doorbar J., Campbell D., Grand R. J. A., Gallimore P. H. 1986; Identification of the human papillomavirus-1a E4 gene products. EMBO Journal 5:355–362
    [Google Scholar]
  7. Dürst M., Gissmann L., Ikenberg H., Zur hausen H. 1983; A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from a different geographical region. Proceedings of the National Academy of SciencesU.S.A. 80:3182–3815
    [Google Scholar]
  8. Gissmann L., Wolnick L., Ikenberg H., Koldovsky U., Schnurch H. G., Zur Hausen H. 1983; Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers. Proceedings of the National Academy of SciencesU.S.A. 80:560–563
    [Google Scholar]
  9. Jenkins D., Tay S. K., McCance D. J., Campion M. J., Clarkson T. K., Singer A. 1986; Histological and immunocytochemical study of cervical intraepithelial neoplasia (CIN) with associated HPV-6 and HPV-16 infection. Journal of Clinical Pathology 39:1177–1180
    [Google Scholar]
  10. Köhler G., Howe S. C., Milstein C. 1976; Fusion between immunoglobulin-secreting and non-secreting myeloma cell lines. European Journal of Immunology 6:292–295
    [Google Scholar]
  11. McCance D. J. 1986; Human papillomaviruses and cancer. Biochimica et biophysica acta 823:195–205
    [Google Scholar]
  12. McCance D. J., Campion M. J., Clarkson P. K., Chesters P. M., Jenkins D., Singer A. 1985; The prevalence of human papillomavirus type 16 DNA sequences in cervical intraepithelial neoplasia and invasive carcinoma of the cervix. British Journal of Obstetrics and Gynaecology 92:1101–1105
    [Google Scholar]
  13. McCance D. J., Lowe D., Simmons P., Thomson J. P. S. 1986; Human papillomavirus in condylomata acuminata of the anus. Journal of Clinical Pathology 39:927
    [Google Scholar]
  14. Stanley K. K., Luzio J. P. 1984; Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins. EMBO Journal 3:1429–1434
    [Google Scholar]
  15. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of SciencesU.S.A. 76:4350–4354
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-1-69
Loading
/content/journal/jgv/10.1099/0022-1317-70-1-69
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error