1887

Abstract

SUMMARY

Antigenic variants of tick-borne encephalitis virus were selected by the use of six neutralizing monoclonal antibodies (MAbs), each defining a different epitope of the envelope glycoprotein E. These variants were characterized with respect to antigenic changes by analysing the binding of each of 18 precisely mapped MAbs in ELISA and haemagglutination inhibition tests. The results yielded information about interrelations between epitopes exceeding that obtained previously from competitive binding studies. In addition, variants were tested for their specific haemagglutination activities, which revealed a significant reduction of this functional activity in one of the variants.

Keyword(s): MAbs , neutralization and TBE virus
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-1-219
1989-01-01
2022-09-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/1/JV0700010219.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-1-219&mimeType=html&fmt=ahah

References

  1. Blondel B., Crainic R., Fichot O., Dufraisse G., Candrea A., Diamond D., Girard M., Horaud F. 1986; Mutations conferring resistance to neutralization with monoclonal antibodies in type 1 poliovirus can be located outside or inside the antibody-binding site. Journal of Virology 57:81–90
    [Google Scholar]
  2. Brinton M. A. 1986; Replication of flaviviruses. In The Togaviridae and Flaviviridae327–374 Schlesinger S., Schlesinger M. J. New York & London: Plenum Press;
    [Google Scholar]
  3. Brown L. E., Ffrench R. A., Gawler J. M., Jackson D. C., Dyall-Smith M. L., Anders E. M., Tregear G. W., Duncan L., underwood P. A., white D. O. 1988; Distinct epitopes recognized by I-Ad-restricted T-cell clones within antigenic site E on influenza virus hemagglutinin. Journal of Virology 62:305–312
    [Google Scholar]
  4. Clarke D. H., Casals J. 1958; Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  5. Heinz F. X. 1986; Epitope mapping of flavivirus glycoproteins. Advances in Virus Research 31:103–168
    [Google Scholar]
  6. Heinz F. X., Kunz C. 1981; Homogeneity of the structural glycoprotein from European isolates of tick-borne encephalitis virus: comparison with other flaviviruses. Journal of General Virology 57:263–274
    [Google Scholar]
  7. Heinz F. X., Berger R., Tuma W., Kunz C. 1983; A topological and functional model of epitopes on the structural glycoprotein of tick-borne encephalitis virus defined by monoclonal antibodies. Virology 126:525–537
    [Google Scholar]
  8. Heinz F. X., Tuma W., Guirakhoo F., Kunz C. 1986; A model study of the use of monoclonal antibodies in capture enzyme immunoassays for antigen quantification exploiting the epitope map of tick-borne encephalitis virus. Journal of Biological Standardization 14:133–141
    [Google Scholar]
  9. Iorio R. M., Bratt M. A. 1985; Selection of unique antigenic variants of Newcastle disease virus with neutralizing monoclonal antibodies and anti-immunoglobulin. Proceedings of the National Academy of SciencesU.S.A. 82:7106–7110
    [Google Scholar]
  10. Mandl C. W., Guirakhoo F., Holzmann H., Heinz F. X., Kunz C. 1989; Antigenic structure of the flavivirus envelope protein E at the molecular level using tick-borne encephalitis virus as a model. Journal of Virology (in press)
    [Google Scholar]
  11. Page S. G., Mosser A. G., Hogle J. M., Filman D. J., Rueckert R. R., Chow N. 1988; Three-dimensional structure of poliovirus serotype 1 neutralizing determinants. Journal of Virology 62:1781–1794
    [Google Scholar]
  12. Rice C. M., Strauss E. G., Strauss J. H. 1986; Structure of the flavivirus genome. In The Togaviridae and Flaviviridae279–320 Schlesinger S., Schlesinger M. J. New York & London: Plenum Press;
    [Google Scholar]
  13. yewdell J. W., Caton A. J., Gerhard W. 1986; Selection of influenza A virus adsorptive mutants by growth in the presence of a mixture of monoclonal antihemagglutinin antibodies. Journal of Virology 57:623–628
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-1-219
Loading
/content/journal/jgv/10.1099/0022-1317-70-1-219
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error