1887

Abstract

SUMMARY

Serological analyses of several different cytoplasmic polyhedrosis viruses (CPVs), including two type 1 CPVs from , type 1 CPV from , type 12 CPV from , type 2 CPV from , type 5 CPV from and type 5 CPV from , demonstrated a close correlation between the antigenic properties of the polyhedrin or virus particle structural proteins and the genomic dsRNA electropherotypes. The dsRNAs of these viruses were analysed by electrophoresis in 3% and 10% polyacrylamide gels with a discontinuous Tris–HC1/Tris–glycine buffer system or by 1% agarose gel electrophoresis using a continuous Tris–acetate–EDTA buffer system. Electrophoretic analysis in agarose gels was found to be the most suitable for the classification of CPV isolates into electropherotypes, and the results obtained showed a close correlation with the observed antigenic relationships between different virus isolates. However, electrophoretic analysis in 10% polyacrylamide gels was most sensitive for the detection of intra-type variation and the presence of mixed virus isolates.

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1989-01-01
2024-04-18
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References

  1. Appleton J. A., Letchworth G. J. 1983; Monoclonal antibody analysis of serotype restricted and unrestricted bluetongue viral antigenic determinants. Virology 124:286–299
    [Google Scholar]
  2. Bodkin D. K., Knudson D. L. 1986; Genetic relatedness of Palyam serogroup viruses by RNA-RNA hybridization. Journal of General Virology 67:683–691
    [Google Scholar]
  3. Both G. W., Siegman L. J., Bellamy A. R., Ikegami N., Shatkin A. J., Furuichi Y. 1984; Comparative sequence analysis of rotavirus genome segment 6-the gene specifying viral subgroups 1 and 2. Journal of Virology 51:97–101
    [Google Scholar]
  4. Brown S. E., Gonzalez H. A., Bodkin D. K., Tesh R. B., Knudson D. L. 1988; Intra- and inter-serogroup genetic relatedness of orbiviruses. II. Blot hybridization and reassortment in vitro of epizootic haemorrhagic disease serogroup, bluetongue type 10 and Pata viruses. Journal of General Virology 69:135–147
    [Google Scholar]
  5. Clarke I. N., Mccrae M. A. 1982; Structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates. Infection and Immunity 36:492–497
    [Google Scholar]
  6. Crook N. E., Payne C. C. 1980; Comparison of three methods of ELISA for baculoviruses. Journal of General Virology 46:29–37
    [Google Scholar]
  7. England T. E., Uhlenbeck O. C. 1978; 3′ termini labelling of RNA T4 RNA ligase. Nature London: 275560–561
    [Google Scholar]
  8. England T. E., Bruce A. G., Uhlenbeck O. C. 1980; Specific labeling of 3′ termini of RNA with T4 RNA ligase. Methods in Enzymology 65:65–74
    [Google Scholar]
  9. Francki R. I. B., Boccardo G. 1983; The plant Reoviridae. In The Reoviridae505–553 Joklik. W. K. New York: Plenum Press;
    [Google Scholar]
  10. Galinski M. W., Stanik V. H., Rohrmann G. F., Beaudreau G. S. 1983; Comparison of sequence diversity in several cytoplasmic polyhedrosis viruses. Virology 130:372–380
    [Google Scholar]
  11. Gonzalez H. A., Knudson D. L. 1987; Genetic relatedness of Corriparta serogroup viruses. Journal of General Virology 68:661–672
    [Google Scholar]
  12. Gonzalez H. A., Knudson D. L. 1988; Intra- and inter-serogroup genetic relatedness of orbiviruses. I. Blot hybridization of viruses of Australian serogroups. Journal of General Virology 69:125–134
    [Google Scholar]
  13. Gorman B. M., Taylor J., Walker P. J. 1983; Orbiviruses. In The Reoviridae287–357 Joklik W. K. New York: Plenum Press;
    [Google Scholar]
  14. Gould A. R. 1987; The complete nucleotide sequence of bluetongue virus serotype 1 RNA3 and a comparison with other geographic serotypes from Australia, South Africa and the United States of America, and with other orbivirus isolates. Virus Research 7:169–183
    [Google Scholar]
  15. Grubman M. J., Appleton J. A., Letchworth G. J. 1983; Identification of bluetongue virus type 17 genome segments coding for polypeptides associated with virus neutralization and intergroup reactivity. Virology 131:355–356
    [Google Scholar]
  16. Harrap K. A., Payne C. C. 1979; The structural properties and identification of insect viruses. Advances in Virus Research 25:273–355
    [Google Scholar]
  17. Holmes I. H. 1983; Rotaviruses. In The Reoviridae359–407 Joklik W. K. New York: Plenum Press;
    [Google Scholar]
  18. Huismans H., Cloete M. 1987; A comparison of different cloned bluetongue virus genome segments as probes for the detection of virus-specified RNA. Virology 158:373–380
    [Google Scholar]
  19. Huismans H., Erasmus B. J. 1981; Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort Journal of Veterinary Research 48:51–58
    [Google Scholar]
  20. Hukuhara T. 1971; Variations in cytoplasmic-polyhedrosis virus. In The Cytoplasmic Polyhedrosis Virus of the Silkworm61–78 Aruga H., Tanada Y. Tokyo: University of Tokyo Press;
    [Google Scholar]
  21. Joklik W. K. 1983; The reovirus particle. In The Reoviridae9–70 Joklik W. K. New York: Plenum Press;
    [Google Scholar]
  22. Knudson D. L. 1986; Nucleic acid hybridisation and zoogeography of some Australian orbiviruses. In Arbovirus Research in AustraliaProceedings of the Fourth Symposium199–206 St George T. D., Kay B. H., Blok J. Brisbane: Queensland Institute for Medical Research;
    [Google Scholar]
  23. Kowalik T. F., Li J. K-K. 1987; The genetic relatedness of United States prototype bluetongue viruses by RNA/RNA hybridization. Virology 158:276–284
    [Google Scholar]
  24. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227680–685
    [Google Scholar]
  25. Mccrae M. A., Mertens P. P. C. 1983; In vitro translation studies on and RNA coding assignments for cytoplasmic polyhedrosis viruses. In Double-Stranded RNA Viruses35–41 Compans R. W., Bishop D. H. L. Amsterdam: Elsevier;
    [Google Scholar]
  26. Maniatis R., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  28. Mertens P. P. C., Sangar D. V. 1985; Analysis of the terminal sequence of the genome segments of four orbiviruses. Virology 140:55–67
    [Google Scholar]
  29. Mertens P. P. C., Pedley S., Cowley J., Burroughs I. N. 1987; A comparison of six different bluetongue virus isolates by cross-hybridization of the dsRNA genome segments. Virology 161:438–447
    [Google Scholar]
  30. Payne C. C. 1976; Biochemical and serological studies of a cytoplasmic polyhedrosis virus from Arctia caja: a naturally-occurring mixture of two virus types. Journal of General Virology 30:357–369
    [Google Scholar]
  31. Payne C. C., Churchill M. P. 1977; The specificity of antibodies to double-stranded (ds)RNA in antisera prepared to three distinct cytoplasmic polyhedrosis viruses. Virology 79:251
    [Google Scholar]
  32. Payne C. C., Harrap K. A. 1977; Cytoplasmic polyhedrosis viruses. In The Atlas of Insect and Plant Viruses105–129 Maramorosch K. New York: Academic Press;
    [Google Scholar]
  33. Payne C. C., Kalmakoff J. 1975; Antibodies to double-stranded RNA: specificity and serum nucleases. Journal of Immunological Methods 9:147–156
    [Google Scholar]
  34. Payne C. C., Mertens P. P. C. 1983; Cytoplasmic polyhedrosis viruses. In The Reoviridae425–492 Joklik W. K. New York: Plenum Press;
    [Google Scholar]
  35. Payne C. C, Rivers C. F. 1976; A provisional classification of cytoplasmic polyhedrosis viruses based on the sizes of the RNA genome segments. Journal of General Virology 33:71–85
    [Google Scholar]
  36. Payne C. C, Tinsley T. W. 1974; The structural proteins and RNA components of a cytoplasmic polyhedrosis virus from Nymphalis io (Lepidoptera: Nymphalidae). Journal of General Virology 25:291–302
    [Google Scholar]
  37. Payne C. C, Piasecka-serafin M., Pilley B. 1977; The properties of two recent isolates of cytoplasmic polyhedrosis viruses. Intervirology 8:155–163
    [Google Scholar]
  38. Payne C. C, Mertens P. P. C, Katagiri K. 1978; A comparative study of three closely related cytoplasmic polyhedrosis viruses. Journal of Invertebrate Pathology 32:310–318
    [Google Scholar]
  39. Payne C. C, Rubinstein R., Crook N. E., Mertens P. P. C. 1983; Serological and molecular studies of variation in cytoplasmic polyhedrosis viruses. In Double-Stranded RNA Viruses253–261 Compans R. W., Bishop D. H. L. Amsterdam: Elsevier;
    [Google Scholar]
  40. Pedley S., Mohamed M. E. H., Mertens P. P. C. 1988; Analysis of genome segments from six different isolates of bluetongue virus using RNA-RNA hybridisation: a generalised coding assignment for bluetongue virus. Virus Research 10:381–390
    [Google Scholar]
  41. Purdy M. A., Ghiasi H., Rao C. D., Roy P. 1985; Complete sequence of bluetongue virus L2 RNA that codes for the antigen recognized by neutralizing antibodies. Journal of Virology 55:826–830
    [Google Scholar]
  42. Purdy M. A., Ritter G. D., Roy P. 1986; Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5, of bluetongue virus serotype 10. Journal of General Virology 67:957–962
    [Google Scholar]
  43. Rohrmann G. F., Bailey T. J., Becker R. R., Beaudreau G. S. 1980; Comparison of the structure of C- and N- polyhedrins from two occluded viruses pathogenic for Orgyia pseudotsugata. Journal of Virology 34:360–365
    [Google Scholar]
  44. Rubinstein R., Harley E. H. 1978; The reproducible alterations of cytoplasmic polyhedrosis virus dsRNA genome patterns on laboratory passage. Virology 84:195–198
    [Google Scholar]
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