1887

Abstract

SUMMARY

Serological analyses of several different cytoplasmic polyhedrosis viruses (CPVs), including two type 1 CPVs from , type 1 CPV from , type 12 CPV from , type 2 CPV from , type 5 CPV from and type 5 CPV from , demonstrated a close correlation between the antigenic properties of the polyhedrin or virus particle structural proteins and the genomic dsRNA electropherotypes. The dsRNAs of these viruses were analysed by electrophoresis in 3% and 10% polyacrylamide gels with a discontinuous Tris–HC1/Tris–glycine buffer system or by 1% agarose gel electrophoresis using a continuous Tris–acetate–EDTA buffer system. Electrophoretic analysis in agarose gels was found to be the most suitable for the classification of CPV isolates into electropherotypes, and the results obtained showed a close correlation with the observed antigenic relationships between different virus isolates. However, electrophoretic analysis in 10% polyacrylamide gels was most sensitive for the detection of intra-type variation and the presence of mixed virus isolates.

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1989-01-01
2022-01-16
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