1887

Abstract

SUMMARY

Attempts to identify and classify distinct potyviruses and their strains have frequently been hampered by the presence of variable proportions of cross-reacting antibodies in antisera. Investigations of reactivities in electroblot immunoassays of 11 polyclonal antisera raised by injection of intact particles of potyviruses produced in different laboratories with 12 distinct potyviruses showed that such cross-reacting antibodies were directed towards the homologous core protein region of potyvirus coat proteins. A simple method was developed to obtain virus-specific antibodies using affinity chromatography. It involved removal of the surface-located, virus-specific N-terminal peptide region from particles of one potyvirus using lysyl endopeptidase, coupling of the truncated coat protein to cyanogen bromide-activated Sepharose gel, and passing antisera to different potyviruses through the column. Antibodies that did not bind to the column were found to be highly specific.

Keyword(s): coat proteins , potyviruses and serology
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1989-01-01
2022-08-08
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References

  1. Allison R. F., Dougherty W. G., Parks T. D., Willis L., Johnston R. E., Kelly M. E., Armstrong F. B. 1985; Biochemical analysis of the capsid protein gene and capsid protein of tobacco etch virus: N-terminal amino acids are located on the virion’s surface. Virology 147:309–316
    [Google Scholar]
  2. Barnett O. W., Randles J. W., Burrows P. M. 1987; Relationships among Australian and North American isolates of bean yellow mosaic potyvirus subgroup. Phytopathology 77:791–799
    [Google Scholar]
  3. Domier L. L., Franklin K. M., Shahabuddin M., Hellman G. M., Overmeyer J. H., Hiremath S. T., Siaw M. E. E., Lomonossoff G. P., Shaw J. G., Rhoads R. E. 1986; The nucleotide sequence of tobacco vein mottling virus. Nucleic Acids Research 14:5417–5430
    [Google Scholar]
  4. Dougherty W. G., Willis L., Johnston R. F. 1985; Topographic analysis of tobacco etch virus capsid protein epitopes. Virology 144:66–72
    [Google Scholar]
  5. Ehlers U, Paul H. L. 1986; Characterization of the coat proteins of different types of barley yellow mosaic virus by polyacrylamide gel electrophoresis and electro-blot immunoassay. Journal of Phytopathology 115:294–303
    [Google Scholar]
  6. Francki R. I. B. 1983; Current problems in plant virus taxonomy. In A Critical Appraisal of Viral Taxonomy63–104 Matthews R. E. F. Boca Raton: CRC Press;
    [Google Scholar]
  7. Francki R. I. B., Milne R. G., Hatta T. 1985; Atlas of Plant Viruses. I, II Boca Raton: CRC Press;
    [Google Scholar]
  8. Geysen H. M., Meloen R. H., Barteling S. I. 1984; Use of peptide synthesis to probe viral antigens for epitopes to a resolution of single amino acids. Proceedings of the National Academy of SciencesU.S.A. 81:3998–4002
    [Google Scholar]
  9. Gough K. H., Shukla D. D. 1981; Coat protein of potyviruses 1. Comparison of the four Australian strains of sugarcane mosaic virus. Virology 111:455–462
    [Google Scholar]
  10. Harrison B. D. 1985; Usefulness and limitations of the species concept for plant viruses. Intervirology 25:71–78
    [Google Scholar]
  11. Hewish D. R., Shukla D. D., Gough K. H. 1986; The use of biotin conjugated antisera in immunoassays for plant viruses. Journal of Virological Methods 13:79–85
    [Google Scholar]
  12. Hiebert E., Tremaine J. H., Ronald W. P. 1984; The effect of limited proteolysis on the amino acid composition of five potyviruses and on the serological reaction and peptide maps of the tobacco etch virus capsid protein. Phytopathology 74:411–416
    [Google Scholar]
  13. Hill J. H., Benner H. I. 1980; Properties of soybean mosaic virus and its isolated protein. Phytopathologische Zeitschrift272–281
    [Google Scholar]
  14. Hollings M., Brunt A. A. 1981a; Potyviruses. In Handbook of Plant Virus Infections: Comparative Diagnosis731–807 Kurstak E. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  15. Hollings M, Brunt A. A. 1981b; Potyvirus group. CMI/AAB Descriptions of Plant Viruses245
    [Google Scholar]
  16. Hunst P. L., Tolin S. A. 1982; Isolation and composition of two strains of soybean mosaic virus. Phytopathology 72:710–713
    [Google Scholar]
  17. Johnson D. A., Gautsch J. W., Sportsman J. R., Elder J. H. 1984; Improved technique utilizing non-fat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Gene Analysis Techniques 1:3–8
    [Google Scholar]
  18. Johnson G., Garvey J. S. 1977; Improved methods for separation and purification by affinity chromatography. Journal of Immunological Methods 15:29–37
    [Google Scholar]
  19. Koenig R., Bercks R. 1968; Änderungen im heterologen Reaktionsvermögen von Antiseren genen Vertreter der potato virus X-Gruppe im Laufe des Immunisierungsprozesses. Phytopathologische Zeitschrift 61:382–398
    [Google Scholar]
  20. Mcdaniel L. L., Gordon D. T. 1985; Identification of a new strain of maize dwarf mosaic virus. Plant Disease 69:602–607
    [Google Scholar]
  21. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  22. Moghal S. M., Francki R. I. B. 1976; Towards a system for the identification and classification of potyviruses I. Serology and amino acid composition of six distinct viruses. Virology 73:350–362
    [Google Scholar]
  23. Moghal S. M., Francki R. I. B. 1981; Towards a system for the identification and classification of potyviruses. II. Virus particle length, symptomatology and cytopathology of six distinct viruses. Virology 112:210–216
    [Google Scholar]
  24. Moyer J. W., Cali B. B. 1985; Properties of sweet potato feathery mottle virus RNA and capsid protein. Journalof General Virology 66:1185–1189
    [Google Scholar]
  25. O’Donnell I. J., Shukla D. D., Gough K. H. 1982; Electro-blot radioimmunoassay of virus-infected plant sap–a powerful new technique for detecting plant viruses. Journal of Virological Methods 4:19–26
    [Google Scholar]
  26. Shukla D. D., Gough K. H. 1979; The use of Protein A, from Staphylococcus aureus, in immune electron microscopy for detecting plant virus particles. Journal of General Virology 45:533–536
    [Google Scholar]
  27. Shukla D. D., Ward C. W. 1988a; Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. Journal of General Virology 69:2703–2710
    [Google Scholar]
  28. Shukla D. D., Ward C W. 1988b; Structure of potyvirus coat proteins and its application in the taxonomy of the potyvirus group. Advances in Virus Research (in press)
    [Google Scholar]
  29. Shukla D. D., Inglis A. S., Mckern N. M., Gough K. H. 1986; Coat protein of potyviruses. 2. Amino acid sequence of coat protein of potato virus Y.. Virology 152:118–125
    [Google Scholar]
  30. Shukla D. D., Gough K. H., Ward C. W. 1987; Coat protein of potyviruses. Comparison of amino acid sequences of the coat proteins of four Australian strains of sugarcane mosaic virus. Archives of Virology 96:59–74
    [Google Scholar]
  31. Shukla D. D., Mckern N. M., Gough K. H., Tracy S. L., Letho S. G. 1988a; Differentiation of potyviruses and their strains by high performance liquid chromatographic peptide profiling of coat proteins. Journal of General Virology 69:493–502
    [Google Scholar]
  32. Shukla D. D., Strike P. M., Tracy S. L., Gough K. H., Ward C. W. 1988b; The N and C termini of the coat proteins of potyviruses are surface-located and the N terminus contains the major virus-specific epitopes. Journal of General Virology 69:1497–1508
    [Google Scholar]
  33. Shukla D. D., Tosic M., Jilka J., Ford R. E., Toler R. W., Langham M. A. C. 1989; Taxonomy of potyviruses infecting maize, sorghum and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology (in press)
    [Google Scholar]
  34. Tosic M., Ford R. E., Moline H. E., Mayhew D. E. 1974; Comparison of techniques for purification of maize dwarf and sugarcane mosaic viruses. Phytopathology 64:439–442
    [Google Scholar]
  35. Vance V. B., Beachy R. N. 1984; Translation of soybean mosaic virus RNA: evidence of protein processing. Virology 132:271–281
    [Google Scholar]
  36. Van Regenmortel M. H. V. 1982; Serology and Immunochemistry of Plant Viruses. New York & London: Academic Press;
    [Google Scholar]
  37. Van Regenmortel M. H. V., Von Wechmar M. B. 1970; A re-examination of the serological relationship between tobacco mosaic virus and cucumber virus 4.. Virology 41:330–338
    [Google Scholar]
  38. Von Baumgarten G., Ford R. E. 1981; Purification and partial characterization of maize dwarf mosaic virus strain A. Phytopathology 71:36–41
    [Google Scholar]
  39. Walkey D. G. A., Webb M. J. W. 1984; The use of a simple electron microscope serology procedure to observe relationships of seven potyviruses. Phytopathologische Zeitschrift 110:319–327
    [Google Scholar]
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