1887

Abstract

SUMMARY

The nucleotide sequence of RNA 1 of the Fny strain (Subgroup I) of cucumber mosaic virus (CMV) was determined and compared at both the nucleic acid and protein levels with the corresponding sequence of RNA 1 of the Q strain (Subgroup II) of CMV. Fny-CMV RNA 1 consisted of 3357 nucleotides and contained a single long open reading frame (ORF) of 2979 nucleotides, whereas Q-CMV RNA 1 consists of 3389 nucleotides and contains a single ORF of 2973 nucleotides. The levels of sequence homology between the two RNAs were 76% at the nucleotide level and 85% at the protein level. These homologies were distributed widely over the molecules, with 45% of the non-conservative differences in amino acid sequence located between amino acids 503 and 705, and another 15% of the differences located between amino acids 224 and 298. While the C-terminal 141 amino acids contain more basic than acidic amino acids, the region of greatest amino acid sequence heterogeneity, amino acids 503 to 600, contained a preponderance of acidic amino acids in the putative translation products of RNAs 1 of both Q-CMV and Fny-CMV. The last 180 nucleotides of the 3′-terminal non-coding region of Fny-CMV RNAs 1 and 2 were 96% homologous, whereas the sequence homology between Fny-CMV RNA 1 and Q-CMV RNA 1 was 64% in this region. Furthermore, the tRNA-like secondary structures formed by the 3′-terminal non-coding regions of Fny-CMV RNAs 1 and 2 were virtually identical. By contrast, there was only 84% sequence homology between the 5′-terminal non-coding regions of these two RNAs and 81% sequence homology between the 5′-terminal non-coding regions of Q-CMV RNA 1 and Fny-CMV RNA 1. The non-equivalent divergence in the non-coding regions of these RNAs, as well as possible functions for the translation product of RNA 1, are discussed.

Keyword(s): CMV , nucleotide sequence and RNA 1
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-1-1
1989-01-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/1/JV0700010001.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-1-1&mimeType=html&fmt=ahah

References

  1. Ahlquist P. 1986; In vitro transcription of infectious viral RNA from cloned cDNA. Methods in Enzymology 118:709–716
    [Google Scholar]
  2. Davies C., Symons R. H. 1988; Further implications for the evolutionary relationships between tripartite plant viruses based on cucumber mosaic virus RNA 3. Virology 165:216–224
    [Google Scholar]
  3. Dayhoff M. O., Eck R. V., Park C. M. 1972; A model of evolutionary change in proteins. In Atlas of Protein Sequence and Structure 589–99 Dayhoff M. O. Washington, D.C: National Biomedical Research Foundation;
    [Google Scholar]
  4. Devergne J. C., Cardin L. 1975; Relations serologiques entre cucumovirus (CMV, TAV, PSV). Annales de Phytopathologie 7:255–276
    [Google Scholar]
  5. Edwards M. C., Gonsalves D. 1983; Grouping of seven biologically defined isolates of cucumber mosaic virus by peptide mapping. Phytopathology 73:1117–1120
    [Google Scholar]
  6. Edwards M. C., Gonsalves D., Prowidenti R. 1983; Genetic analysis of cucumber mosaic virus in relation to host resistance: location of determinants for pathogenicity to certain legumes and Lactuca saligna. Phytopathology 73:269–273
    [Google Scholar]
  7. Galibert F., Mandart E., Fitoussi F., Tiollais P., Charnay P. 1979; Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature London: 281646–650
    [Google Scholar]
  8. Garcia-arenal F., Zaitlin M., Palukaitis P. 1987; Nucleotide sequence analysis of six satellite RNAs of cucumber mosaic virus: primary sequence and secondary structure alterations do not correlate with differences in pathogenicity. Virology 158:339–347
    [Google Scholar]
  9. Gonda T. J., Symons R. H. 1978; The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: its application to several strains of cucumo viruses. Virology 88:361–370
    [Google Scholar]
  10. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  11. Hanada K., Tochihara H. 1980; Genetic analysis of cucumber mosaic, peanut stunt and chrysanthemum mild mottle viruses. Annals of the Phytopathological Society of Japan 46:159–168
    [Google Scholar]
  12. Haseloff J., Symons R. H. 1981; Chrysanthemum stunt viroid: primary sequence and secondary structure. Nucleic Acids Research 9:2741–2752
    [Google Scholar]
  13. Haseloff J., Gqelet P., Zimmern D., Ahlquist P., Dasgupta R., Kaesberg P. 1984; Striking similarities in amino acid sequence among nonstructural proteins encoded by RNA viruses that have dissimilar genomic organization. Proceedings of the National Academy of SciencesU.S.A. 81:4358–4362
    [Google Scholar]
  14. Hodgman T. C. 1988; A new superfamily of replicative proteins. Nature London: 33322–23
    [Google Scholar]
  15. Jessee J. 1986; New subcloning efficiency competent cells: >1 × 106 transformants/μg. Focus 849–10 Gaithersburg: Bethesda Research Laboratories;
    [Google Scholar]
  16. Kamer G., Argos P. 1984; Primary structural comparison of RNA-dependent RNA polymerases from plant, animal and bacterial viruses. Nucleic Acids Research 12:7269–7282
    [Google Scholar]
  17. Kiberstis P. A., Loesch-Fries L. S., Hall T. C. 1981; Viral protein synthesis in barley protoplasts inoculated with native and fractionated brome mosaic virus RNA. Virology 112:804–808
    [Google Scholar]
  18. Lakshman D. K., Gonsalves D. 1985; Genetic analyses of the large lesion mutants of two cucumber mosaic virus strains. Phytopathology 75:758–762
    [Google Scholar]
  19. Liss L. R. 1987; New M13 host: DH5αF′ competent cells. Focus 9313 Gaithersburg: Bethesda Research Laboratories;
    [Google Scholar]
  20. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Marchoux G., Marou J., Quiot J.-B. 1974; Complémentation entre ARN de différentes souches du virus de la mosaique du concombre. Mise en évidence d’une interaction entre deux ARN pour déterminer un type de symptôme. Comptes rendus hebdomadaires des séances de l’Académie des sciences, Série D 278:1943–1946
    [Google Scholar]
  22. Messing J. 1979; A multipurpose cloning system based on the single-stranded DNA bacteriophage M13. Recombinant DNA Technical Bulletin Bethesda: NIH; Publication No. 79–99 2243–48
    [Google Scholar]
  23. Messing I. 1983; New M13 vectors for cloning. Methods in Enzymology 101:20–78
    [Google Scholar]
  24. Nassuth A., Bol J. F. 1983; Altered balance of the synthesis of plus- and minus-strand RNAs induced by RNAs 1 and 2 of alfalfa mosaic virus in the absence of RNA 3. Virology 124:75–85
    [Google Scholar]
  25. Norrander J., Kempe T., Messing J. 1983; Construction of improved M13 vectors using oligonucleotide-directed mutagenesis. Gene 16:101–106
    [Google Scholar]
  26. Palukaitis P., Zaitlin M. 1984; Satellite RNAs of cucumber mosaic virus: characterization of two new satellites. Virology 132:426–435
    [Google Scholar]
  27. Pasek M., Goto T., Gilbert W., Zink B., Schaller H., Mackay P., Leadbetter G., Murray K. 1979; Hepatitis B virus genes and their expression in E. coli. Nature London: 282575–579
    [Google Scholar]
  28. Peden K. W. C., Symons R. H. 1973; Cucumber mosaic virus contains a functionally divided genome. Virology 53:487–492
    [Google Scholar]
  29. Piazzolla P., Diaz-Ruiz J. R., Kaper J. M. 1979; Nucleic acid homologies of eighteen cucumber mosaic virus isolates determined by competition hybridization. Journal of General Virology 45:361–369
    [Google Scholar]
  30. Poncz M., Solowiejczyk D., Ballantine M., Schwartz E., Surrey S. 1982; “Nonrandom” DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method. Proceedings of the National Academy of SciencesU.S.A. 79:4298–4302
    [Google Scholar]
  31. Rao A. T. N., Francki R. I . B. 1982; Distribution of determinants for symptom production and host range on the three RNA components of cucumber mosaic virus. Journal of General Virology 61:197–205
    [Google Scholar]
  32. Rezaian M. A., Williams R. H. V., Gould A. R., Symons R. H. 1984; Nucleotide sequence of cucumber-mosaic-virus RNA 2 reveals a translation product significantly homologous to corresponding proteins of other viruses. European Journal of Biochemistry 143:277–284
    [Google Scholar]
  33. Rezaian M. A., Williams R. H. V., Symons R. H. 1985; Nucleotide sequence of cucumber mosaic virus RNA 1. Presence of a sequence complementary to part of the viral satellite RNA and homologies with other viral RNAs. European Journal of Biochemistry 150:331–339
    [Google Scholar]
  34. Rizzo T. M., Palukaitis P. 1988; Nucleotide sequence and evolutionary relationships of cucumber mosaic virus (CMV) strains: CMV RNA 2. Journal of General Virology 69:1777–1787
    [Google Scholar]
  35. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of SciencesU.S.A. 74:5463–5467
    [Google Scholar]
  36. Sanger F., Coulson A. R., Barrell B. G., Smith A. I. H., Roe B. A. 1980; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  37. Schwinghamer M. W., Symons R. H. 1977; Translation of the four major RNA species of cucumber mosaic virus in plant and animal cell-free systems and in toad oocytes. Virology 79:88–108
    [Google Scholar]
  38. yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13 mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  39. Zitter T. A., Gonsalves D. 1986; Analysis of pseudorecombinants of two strains of cucumber mosaic virus differing in symptom expression and aphid transmissibility in squash. Proceedings of the Workshop on Epidemiology of Plant Virus Diseases, Orlando, FloridaAugust 6–8 198619–21
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-1-1
Loading
/content/journal/jgv/10.1099/0022-1317-70-1-1
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error