1887

Abstract

Summary

Morphological transformation of normal mouse embryo cells by murine sarcoma virus () and subsequent development of murine sarcoma virus growth were investigated using cytosine arabinoside (ara-C) and X-irradiation. Inhibition of DNA synthesis, cell division and transformation by ara-C were all reversible by deoxycytidine. The susceptibility of mouse embryo cells to infection and morphological transformation was most sensitive to ara-C during the first 6 hr after the virus-cell encounter. Sensitivity decreased with time, and by 24 to 48 hr virtually all infected cells had become transformed and completed the virus growth cycle, despite ara-C treatment. Irradiation of transformed, virus-producing cells with either 5000 or 100,000 had little effect on the ability of infected cells to produce murine sarcoma virus 24 hr later, and about 10% irradiated cells were still producing virus after 48 hr. Radiation survival and heat (37°) inactivation data for murine sarcoma virus () are presented. The results indicate that the successful infection and morphological transformation of normal mouse embryo cells by murine sarcoma virus () requires a DNA synthetic event immediately after the virus-cell encounter and, that once initiated, successful virus growth no longer depends on DNA synthesis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-7-3-249
1970-06-01
2019-11-19
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-7-3-249
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error