1887

Abstract

SUMMARY

After a first exposure to single-stranded polyriboinosinic and polyribocytidylic acids, which initiates interferon production, rabbit kidney cultures become refractory for about 3 days to further stimulation with polyriboinosinic and polyribocytidylic acids. However, when polyriboinosinic and polyribocytidylic acids are left in contact with the cells for several days, the interferon response is not restored to normal, as appears from a gradually decreasing production rate and a decreasing responsiveness to a higher dose. This is interpreted as indicating that refractoriness is not merely due to exhaustion of interferon precursors, but also to a blocking of synthesis of interferon or interferon precursors. Small doses of polyriboinosinic and polyribocytidylic acids which did not induce measurable interferon, but which did stimulate resistance to virus, caused a hyper-reactivity to a second exposure to a high dose of polyriboinosinic and polyribocytidylic acids. It was concluded that in rabbit kidney cells, refractoriness is not correlated with the antiviral state resulting from stimulation of the interferon mechanism.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-7-3-225
1970-06-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/7/3/JV0070030225.html?itemId=/content/journal/jgv/10.1099/0022-1317-7-3-225&mimeType=html&fmt=ahah

References

  1. Burke D. C., Buchan A. 1965; Interferon production in chick embryo cells. I. Production by ultraviolet-inactivated virus. Virology 26:28
    [Google Scholar]
  2. Burke D. C., Isaacs A. 1958; Some factors affecting the production of interferon. British Journal of Experimental Pathology 39:452
    [Google Scholar]
  3. Cantell K., Paucker K. 1963; Quantitative studies on viral interference in suspended L cells. IV. Production and assay of interferon. Virology 21:11
    [Google Scholar]
  4. Field A. K., Tytell A. A., Lampson G. P., Hilleman M. R. 1967; Inducers of interferon and host resistance. II. Multistranded synthetic polynucleotide complexes. Proceedings of the National Academy of Sciences of the United States of America 58:1004
    [Google Scholar]
  5. Friedman R. M. 1966; Effect of interferon treatment on interferon production. Journal of Immunology 96:872
    [Google Scholar]
  6. Huppert J., Hillova J., Gresland L. 1969; Viral RNA synthesis in chicken cells infected with ultraviolet irradiated Newcastle disease virus. Nature; London: 2231015
    [Google Scholar]
  7. Isaacs A., Burke D. C. 1958; Mode of action of interferon. Nature; London: 1821073
    [Google Scholar]
  8. Levy H. B., Buckler C. E., Baron S. 1966; Effect of interferon on early interferon production. Science; New York: 1521274
    [Google Scholar]
  9. Lindenmann J., Bucke D. C., Issacs A. 1957; Studies on the production, mode of action and properties of interferon. British Journal of Experimental Pathology 38551
    [Google Scholar]
  10. Lockart R. Z. 1963; Production of an interferon by L cells infected with Western equine encephalomyelitis virus. Journal of Bacteriology 85:556
    [Google Scholar]
  11. Margolis S. A., Levy H. B. 1969; The action of poly-In.Cn on the RNA metabolism of cultured cells. Proceedings of the Second International Symposium on Antiviral Substances, New York Academy of Sciences In the Press
    [Google Scholar]
  12. Paucker K., Boxaca M. 1967; Cellular resistance to induction of interferon. Bacteriological Reviews 31:145
    [Google Scholar]
  13. Skehel J. J., Burke D. C. 1968; A temperature-sensitive event in interferon production. Journal of General Virology 3:191
    [Google Scholar]
  14. Vilcek J. 1969; Metabolic determinants of the induction of interferon by a synthetic double-stranded polynucleotide in rabbit kidney cells. Proceedings of the Second International Symposium on Antiviral Substances, New York Academy of Sciences In the Press
    [Google Scholar]
  15. Vilcek J. 1962; Studies on an interferon from tick-borne encephalitis virus-infected cells (IF). IV. Comparison of IF with interferon from influenza virus-infected cells. Acta Virologica 6:144
    [Google Scholar]
  16. Vilcek J., Rada B. 1962; Studies on an interferon from tick-borne encephalitis virus-infected cells (IF). III. Antiviral action of IF. Acta Virologica 6:9
    [Google Scholar]
  17. Youngner J. S., Hallum J. V. 1969; Inhibition of induction of interferon synthesis in L cells pretreated with interferon. Virology 37:473
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-7-3-225
Loading
/content/journal/jgv/10.1099/0022-1317-7-3-225
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error