@article{mbs:/content/journal/jgv/10.1099/0022-1317-7-3-211, author = "Way, Hilary J. and Garwes, D. J.", title = "Serum Accessory Factors in the Measurement of Arbovirus Neutralization Reactions", journal= "Journal of General Virology", year = "1970", volume = "7", number = "3", pages = "211-223", doi = "https://doi.org/10.1099/0022-1317-7-3-211", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-7-3-211", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Factors in normal serum which enhance Semliki Forest virus neutralization by specific immune guinea-pig serum have been demonstrated in a range of normal domestic and laboratory animals. The factors alfecting the in vitro assay system for accessory factors have been determined and enhancement of neutralization by normal guinea-pig serum in other virus/cell systems has been demonstrated. Accessory factors are denatured by various physical and chemical procedures but survive freeze-drying. The effect of ageing on accessory factors in vivo in normal and germ-free rats and rabbits and in normal calves, lambs, guinea-pigs and mice revealed marked species differences but showed no difference between normal and germ-free animals of the same species. There was no correlation between haemolytic complement and accessory factor levels present in a serum, except in rats, although the two complexes had several properties in common.", }