1887

Abstract

SUMMARY

Balb/c mouse embryonic fibroblasts were transformed by the murine sarcoma virus (MSV) ( strain). These cells have a disorderly growth pattern. When grown in soft agar 61 % of them formed colonies. They contained ‘C’ type MSV particles and the transplantation antigen of the murine leukosis group of viruses. After 200 passages with interferon in the tissue culture medium, a cell line (MSV-IF) emerged. This cell line recovered orderly growth and only 0·5% of the cells gave rise to colonies in soft agar. It contained about ten times more ‘C’ type particles than the original MSV cells. These particles harboured the MSV and leukaemia genome. The cells also retained the group and transplantation antigens. MSV-IF cells were completely resistant to exogenous interferon. When challenged with Newcastle disease virus they produced about ten times more interferon than the original MSV cells. Newcastle disease virus did not induce a refractory state to further interferon induction. When challenged five times every 48 hr the cells always produced a significant amount of interferon. It was postulated that this MSV-interferon cell line, unlike the original cell population, was not able to produce or use a repressor of interferon synthesis. In addition, the transformed cells recovered, to some degree, properties of the original normal cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-7-3-203
1970-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/7/3/JV0070030203.html?itemId=/content/journal/jgv/10.1099/0022-1317-7-3-203&mimeType=html&fmt=ahah

References

  1. Chany C., Vignal M. 1968; Étude du mécanisme de l’état réfractaire des cellules à la production d’interféron après inductions repétées. Compte rendu hebdomadaire des séances de l’Académie des sciences 367:1798
    [Google Scholar]
  2. Finter N. B. 1964; Protection of mice by interferon against systemic virus infections. British Medical Journal 2:981
    [Google Scholar]
  3. Gresser L., Coppey J., Falcoff E., Fontaine D. 1967; Interferon and murine leukemia I. Inhibitory effect of interferon preparations on development of Friend leukemia in mice. Proceedings of the Society for Experimental Biology and Medicine 124:84
    [Google Scholar]
  4. Gresser I., Bourali C., Levy J. P., Fontaine-Brouty-Boye D., Thomas M. T. 1969; Prolongation de la survie des souris inoculées avec des cellules tumorales et traitées avec des préparations d’interfé7rons. Compte rendu hebdomadaire des séances de l’Académie des sciences 268:994
    [Google Scholar]
  5. Hartley J. W., Rowe W. P. 1966; Production of altered cell foci in tissue cultures by defective Moloney sarcoma virus particles. Proceedings of the National Academy of Sciences of the United States of America 55:780
    [Google Scholar]
  6. Huebner R. J., Hartley J. W., Rowe W. P., Lane W. T., Capps W. I. 1966; Rescueofthe defective genome of Moloney sarcoma virus from a non-infectious hamster tumor and the production of pseudotype sarcoma viruses with various murine leukemia viruses. Proceedings of the National Academy of Sciences of the United States of America 56:1164
    [Google Scholar]
  7. Moloney J. B. 1966; A virus-induced rhabdomyosarcoma of mice. National Cancer Institute Monograph 22:139
    [Google Scholar]
  8. Montagnier L., Macpherson I. 1964; Croissance sélective en gélose de cellules de hamster transformées par le virus du polyome. Compterendu hebdomadaire des séances de l’Académie des sciences 258:4117
    [Google Scholar]
  9. Oxman M. N., Black P. H. 1966; Inhibition of SV40 T antigen formation by interferon. Proceedings of the National Academy of Sciences of the United States of America 55:1133
    [Google Scholar]
  10. Paucker K., Boxaca M. 1967; Cellular resistance of induction of interferon. Bacteriological Reviews 31:145
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-7-3-203
Loading
/content/journal/jgv/10.1099/0022-1317-7-3-203
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error