When synchronized chicken embryo cells were infected with Rous sarcoma virus in the the G-1 period of the cell cycle, virus replication was delayed by several hours compared with cells infected in the S period, and virus release began only after mitosis. The delay in replication was not due to a change in the rate of virus adsorption and penetration. There was no delay in replication of Newcastle disease virus, indicating that the cells in G-1 were able to support replication of other RNA viruses. The shortest latent period for RSV (12 to 14 hr) occurred when cells were infected between S and mitosis. The latent period increased for cells infected after the maximum of mitosis. Cells which had been in culture for more than 15 hr rapidly lost their susceptibility to infection by RSV.


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