Three serologically distinct bovine enteroviruses (VG-5–27; vc-65–182; vp-7–19) grew in BHK21 cells to concentrations of approximately 5 × 108 p.f.u./ml. A purification procedure was used which involved sedimentation and gel-filtration through a Sepharose 2B column. The virus particles were spherical (diameter of 27 nm.), had a buoyant density of 1·34 g./ml. in CsCl and a sedimentation coefficient of approximately 165 s. The RNA of each of the virus types had a sedimentation coefficient of 35 s in sucrose-gradients and the base compositions were similar, being characterized by high A (30 %) and low U (21 %).
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