1887

Abstract

SUMMARY

Three serologically distinct bovine enteroviruses (VG-5–27; vc-65–182; vp-7–19) grew in BHK21 cells to concentrations of approximately 5 × 10 p.f.u./ml. A purification procedure was used which involved sedimentation and gel-filtration through a Sepharose 2B column. The virus particles were spherical (diameter of 27 nm.), had a buoyant density of 1·34 g./ml. in CsCl and a sedimentation coefficient of approximately 165 s. The RNA of each of the virus types had a sedimentation coefficient of 35 s in sucrose-gradients and the base compositions were similar, being characterized by high A (30 %) and low U (21 %).

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1970-05-01
2024-12-14
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References

  1. Brown F., Cartwright B. 1963; Purification of radioactive foot-and-mouth disease virus. Nature; London: 1991168
    [Google Scholar]
  2. Brown F., Martin S. J. 1965; Base composition of ribosomal RNA fractions from five mammalian tissue-culture strains. Biochemical Journal 97:20c
    [Google Scholar]
  3. Davidson J. N. 1952; Phosphorus compounds in the cell. 3. The incorporation of radioactive phosphorus into the ribonucleotide fraction of liver tissue. Biochemical Journal 52:599
    [Google Scholar]
  4. Flamm W. G., Bond H. E. 1966; Density-gradient centrifugation of DNA in a fixed-angle rotor: A higher order of resolution. Biochemical et Biophysica Acta 129:310
    [Google Scholar]
  5. Hausen P., Schäfer W. 1962; Untersuchungen ber ein Mausencephalomyelitis virus. Reinigung und physikalischchemische Eigenschaften des Virus. Zeitschrift für Naturforschung 17b:15
    [Google Scholar]
  6. Huck R. 1964; Isolation and classification of viruses from cattle during outbreaks of mucosal or respiratory disease and from herds with reproductive disorders. Journal of Comparative Pathology and Therapeutics 74:346
    [Google Scholar]
  7. Kaighn M. E., Mascarello M. A., Fuerst C. R. 1964; Purification of murine encephalomyocarditis virus. Virology 23:183
    [Google Scholar]
  8. La Plaça M., Portolani M., Lamieri C. 1965; The basis for classification of bovine enteroviruses. Archiv für die gesamte Virusforschung 17:98
    [Google Scholar]
  9. Levintow L., Darnell J. E. 1960; A simplified procedure for purification of large amounts of poliovirus. Journal of Biological Chemistry 235:70
    [Google Scholar]
  10. Macpherson I. A., Stoker M. G. P. 1962; Polyoma transformation of Hamster Cell Clones-an investigation of genetic factors affecting cell competence. Virology 16:147
    [Google Scholar]
  11. Mcferran J. B. 1962; Bovine Enteroviruses. Annals of the New York Academy of Sciences 101:436
    [Google Scholar]
  12. Mcferran J. B., Nelson R., Mccracken J., Ross J. G. 1969; Various types of virus isolated from sheep. Nature; London: 221194
    [Google Scholar]
  13. Markham R. 1959 In The Viruses 2 p 33 Burnet F. M., Stanley W. M. Ed. by New York: Academic Press;
    [Google Scholar]
  14. Mowat G. N., Chapman W. G. 1962; Growth of foot-and-mouth disease virus in a fibroblastic line derived from hamster kidneys. Nature; London: 194253
    [Google Scholar]
  15. Oellermann R. A., Els H. J., Verwoerd D. W. 1967; Purification and Physico-chemical characterisation of Ecbovirus type SA-I. Onderstepoort Journal of Veterinary Research 34:53
    [Google Scholar]
  16. Poison A., Kipps A. 1965; Physico-chemical investigation of an enteric cytopathogenic bovine orphan virus Ecbo SA-l. Archiv für die gesamte Virusforschung 17:488
    [Google Scholar]
  17. Wild T. F., Martin S. J., Brown F. 1968; A study of the heterogeneous 37s RNA induced by foot-and-mouth disease virus. Biochemical Journal 107:395
    [Google Scholar]
  18. Verwoerd D. W., Oellermann R. A., Brockman J., Weiss K. E. 1967; The serological relationship of South African Bovine enterovirus strains (Ecbo SA-i and -n) and the growth characteristics in cell culture of the prototype strain (Ecbo SA-i). Onderstepoort Journal of Veterinary Research 34:41
    [Google Scholar]
  19. Vinograd J., Hearst J. E. 1962; . Fortschritte der Chemie organischer Naturstoffe 20:373
    [Google Scholar]
  20. Yamada S. 1965; Studies on bovine enteroviruses. Japanese Journal of Veterinary Science 27:171
    [Google Scholar]
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