1887

Abstract

Summary

Three serologically distinct bovine enteroviruses (-5-27; -65-182; -7-19) grew in BHK21 cells to concentrations of approximately 5 × 10 p.f.u./ml. A purification procedure was used which involved sedimentation and gel-filtration through a Sepharose 2 B column. The virus particles were spherical (diameter of 27 nm.), had a buoyant density of 1.34 g./ml. in CsCl and a sedimentation coefficient of approximately 165s. The RNA of each of the virus types had a sedimentation coefficient of 35 in sucrose-gradients and the base compositions were similar, being characterized by high A (30%) and low U (21%).

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/content/journal/jgv/10.1099/0022-1317-7-2-103
1970-05-01
2019-10-22
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-7-2-103
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