1887

Abstract

SUMMARY

The addition of either isatin--thiosemicarbazone (IBT) or its -methyl derivative effectively inhibited the multiplication of various isolates of infectious bovine rhinotracheitis virus. Treatment of infected cultures with IBT early or late in the log. phase of replication markedly suppressed the production of infectious virus, indicating that the drug inhibits virus maturation. The time course of formation of infectious virus and of synthesis of viral DNA and viral protein was determined with the inhibitors FUdR and cycloheximide, respectively. In radioisotope experiments, IBT inhibited the incorporation of label into both RNA and DNA at a fairly early stage of the virus growth cycle.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-7-1-55
1970-04-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/7/1/JV0070010055.html?itemId=/content/journal/jgv/10.1099/0022-1317-7-1-55&mimeType=html&fmt=ahah

References

  1. Appleyard G., Hume V. B. M., Westwood J. C. N. 1965; The effect of thiosemicarbazones on the growth of rabbit pox virus in tissue culture. Annals of the New York Academy of Sciences 130:92
    [Google Scholar]
  2. Bach M. K., Magee W. E. 1962; Biochemical effect of isatin β-thiosemicarbazone on development of vaccinia virus. Proceedings of the Society for Experimental Biology and Medicine 110:565
    [Google Scholar]
  3. Bauer D. J. 1955; The antiviral and synergic actions of isatin thiosemicarbazone and certain phenoxypyrimidines in vaccinia infection in mice. British Journal of Experimental Pathology 36:105
    [Google Scholar]
  4. Bauer D. J. 1965; Clinical experience with the antiviral drug Marboran (1-methylisatin 3-thiosemicarbazone). Annals of the New York Academy of Sciences 130:110
    [Google Scholar]
  5. Bauer D. J., Apostolov K. 1966; Adenovirus multiplication: Inhibition by methisazone. Science; New York: 154796
    [Google Scholar]
  6. Burton K. 1956; A study of the conditions and mechanisms of diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315
    [Google Scholar]
  7. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science; New York: 130432
    [Google Scholar]
  8. Easterbrook K. B. 1962; Interference with the maturation of vaccinia virus by isatin β-thiosemicarbazone. Virology 17:245
    [Google Scholar]
  9. Hamada C., Tomoya K., Kaplan A. S. 1966; Serological analysis of some enzymes present in pseudorabies virus-infected and noninfected cells. Virology 28:271
    [Google Scholar]
  10. Hamre D., Bernstein J., Donovick R. 1950; Activity of p-aminobenzaldehyde 3-thiosemicarbazone on vaccinia virus in the chick embryo and in the mouse. Proceedings of the Society for Experimental Biology and Medicine 73:275
    [Google Scholar]
  11. Lowry O.H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin reagent. Journal of Biological Chemistry 236:1141
    [Google Scholar]
  12. Magee W. E., Bach M. K. 1965; Biochemical studies in the antiviral activities of the isatin /9-thiosemicarbazones. Annals of the New York Academy of Sciences 130:80
    [Google Scholar]
  13. Mejbaum W. 1939; Über die Bestimmung kleiner Pentcsemengen, insbesondere in Derivaten der Adenysaure. Zeitschrift für physiologische Chemie 258:117
    [Google Scholar]
  14. Sabina L. R., Parker R. C. 1963; Studies of infectious bovine rhinotracheitis virus. I. Plaque assay and some characteristics in bovine kidney cells. Canadian Journal of Microbiology 9:887
    [Google Scholar]
  15. Sabina L. R., Munro T. W. 1969; Effects of trypsin on replication of parainfluenza-3 virus in HEp-2 cell cultures. Canadian Journal of Microbiology 15:577
    [Google Scholar]
  16. Schmidt G., Thannhauser S. J. 1945; A method for the determination of deoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues. Journal of Biological Chemistry 161:293
    [Google Scholar]
  17. Sheffield F. W., Bauer D. J., Stephenson S. M. 1960; The protection of tissue cultures by isatin fi-thiosemicarbazone from cytopathic effects of certain pox viruses. British Journal of Experimental Pathology 41:638
    [Google Scholar]
  18. Thompson R. L., Minton S. A., Officer J. E., Hitchings G. H. 1953; Effect of heterocyclic and other thiosemicarbazones on vaccinia infection in the mouse. Journal of Immunology 70:229
    [Google Scholar]
  19. Turner W., Bauer D. J., Nimmo-Smith R. H. 1962; Eczema vaccinatum treated with N-Methylisatin β-thiosemicarbazone. British Medical Journal 1:1317
    [Google Scholar]
  20. Warner A. H., Mcclean D. K. 1968; Studies on the biosynthesis and role of diguanosine tetraphosphate during growth and development of Artemia salina . Developmental Biology 18:287
    [Google Scholar]
  21. Woodson B. 1968; Recent progress in poxvirus research. Bacteriological Reviews 32:127
    [Google Scholar]
  22. Woodson B., Joklik W. K. 1965; The inhibition of vaccinia virus multiplication by isatin-/?-thiosemicarbazone. Proceedings of the National Academy of Sciences of the United States of America 54:946
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-7-1-55
Loading
/content/journal/jgv/10.1099/0022-1317-7-1-55
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error