1887

Abstract

SUMMARY

Six different Rous mammalian tumour lines were studied for the occurrence of virus specific antigens, the presence of the viral genome and the synthesis of infectious and non-infectious particles. Four Rous tumour lines were induced in mice presumably free from latent murine leukemia virus (MLV), three of them by a cloned Schmidt-Ruppin strain of Rous sarcoma virus (-RSV) belonging to sub-group D, the fourth with an uncloned -RSV strain. In addition a Prague(P)-RSV induced mouse tumour line (RVP) and an -RSV induced hamster tumour line (RSH) and -RSV induced chicken tumours were likewise investigated. In all mammalian lines, except for RVP, avian leukosis group specific complementfixing antigen was found. Tumour specific transplantation antigen was demonstrated in all mammalian lines except RSH, whereas no infectious RSV could be detected in any of the mammalian tumours. Likewise electron microscopy revealed no virus particles in more than 9500 cell sections of RSV-stu tumours, but the viral genome was shown to be present by transfer of the tumour to chickens. In the RVP line virus particles were detected resembling typical murine leukemia virus while in the RSH line particles were found morphologically similar to the latent hamster virus described by Bernhard & Tournier (1964).

The interpretation and significance of the findings are discussed.

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1970-04-01
2022-01-20
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References

  1. Altaner C., Svec F. 1966; Virus production in rat tumors induced by chicken sarcoma virus. Journal of the National Cancer Institute 37:745
    [Google Scholar]
  2. Bauer H., Graf Th. 1969; Evidence for the possible existence of two envelope antigenic determinants and corresponding cell receptors for avian tumor viruses. Virology 37:157
    [Google Scholar]
  3. Bauer H., Janda H. G. 1967; Group specific antigen of avian leuksois viruses, virus specificity and relation to an antigen contained in Rous mammalian cells. Virology 33:483
    [Google Scholar]
  4. Bernhard W. 1960; The detection and study of tumor viruses with the electron microscope. Cancer Reseàrch 20:712
    [Google Scholar]
  5. Bernhard W., Oberling C., Vigier P. 1956; L’ultrastructure de particules de virus du sarcome de Rous et leur rapport avec le cytoplasme des cellules tumorales. Bulletin du Cancer 43:407
    [Google Scholar]
  6. Bernhard W., Tournier P. 1964; Infection virale inapparente de cellules de hamsters décelée par la microscopie électronique. Annales de l’Institut Pasteur 107:447
    [Google Scholar]
  7. Buberník J., Bauer H. 1967; Antigenic characteristics of the interaction between Rous sarcoma virus and mammalian cells. Complement-fixing and transplantation antigens. Virology 31:489
    [Google Scholar]
  8. Buberník J., Koldovský P., Svoboda J., Klement V., Dvořák R. 1967; Induction of tumours in mice with three variants of Rous sarcoma virus and studies on the immunobiology of these tumours. Folia Biologica 13:29
    [Google Scholar]
  9. Dalton A., De Harven E., Dmochowski L., Feldman D., Haguenau F., Harris W., Howatson A., Moore D., Pitelka D., Smith K., Uzman B., Zeigel R. 1966; Suggestions for the classification of oncogenic RNA viruses. Journal of the National Cancer Institute 37:395
    [Google Scholar]
  10. Dougherty R. M., Di Stefano H. S. 1965; Virus particles associated with ‘nonproducer’ Rous sarcoma cells. Virology 27:351
    [Google Scholar]
  11. Epstein M. A. 1957; Observations on the Rous virus; fine structure and relation to cytoplasmic vacuoles. British Journal of Cancer 11:268
    [Google Scholar]
  12. Fèbvre H., Rothschild L., Arnoult J., Haguenau F. 1964; In vitro malignant conversion of rat embryonic cell lines with the Bryan strain of Rous sarcoma virus. National Cancer Institute Monograph 17:459
    [Google Scholar]
  13. Goldé A., Latarjet R. 1966; Dissociation, par irradiation, des fonctions oncogènes et infectieuses du virus de Rous, souche Schmidt-Ruppin. Compte rendu hebdomadaire des séances de l’Académie des sciences 262:420 Série D
    [Google Scholar]
  14. Graf Th., Bauer H. 1969; Studies on the relative target size of various functions in the genome of avian tumor viruses. II. International Meeting on Tumor Viruses Royaumont 1969 (in the Press)
    [Google Scholar]
  15. Haguenau F., Beard J. W. 1962; The avian sarcomaleukosis complex; its biology and ultrastructure. In Ultrastructure in Biological Systems 1 pp 1–60 New York, London: Academic Press;
    [Google Scholar]
  16. Hall W. T., Andresen W. F., Sanford K. K., Evans V. J., Hartley J. W. 1967; Virus particles and murine leukemia virus complement-fixing antigen in neoplastic and non-neoplastic cell lines. Science; New York: 15685
    [Google Scholar]
  17. Hanafusa H., Hanafusa T. 1968; Further studies on RSV production from transformed cells. Virology 34:630
    [Google Scholar]
  18. Hartley J. W., Rowe W. P., Capps W. i., Huebner R. J. 1969; Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. Journal of Virology 3:126
    [Google Scholar]
  19. De Harven E. 1964; Virus particles in the thymus of conventional and germ-free mice. Journal of Experimental Medicine 120:857
    [Google Scholar]
  20. Kajima M., Pollard M. 1968; Wide distribution of leukaemia virus in strains of laboratory mice. Nature; London: 218188
    [Google Scholar]
  21. Klement V., Veselý P. 1965; Tumour induction with the Rous sarcoma virus in hamsters and production of infectious Rous sarcoma virus in a heterologous host. Neoplasma 12:147
    [Google Scholar]
  22. Lindberg L. G. 1968; Virus-like particles in Rous rate sarcoma. Acta pathologica et microbiologica scandi-navica 74:189
    [Google Scholar]
  23. Luft H. 1961; Improvements in epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology 9:409
    [Google Scholar]
  24. Millonig G. 1961; Advantages of a phosphate buffer of OsO3 solution in fixation. Proceedings of the 19th Annual Meeting Electron Microscope Society of America p:15
    [Google Scholar]
  25. Rabotti G. F., Bucciarelli E., Dalton A. J. 1966; Presence of particles with the morphology of viruses of the avian leukosis complex in meningeal tumors induced in dogs by Rous sarcoma virus. Virology 29:684
    [Google Scholar]
  26. Rowe W. P., Hartley D. W., Capps W. I. 1966; Tissue culture and serologic studies of mouse leukemia viruses. National Cancer Institute Monograph 22:15
    [Google Scholar]
  27. Schäfer W., Seifert E. 1968; Production of a potent complement-fixing murine leukemia virus antiserum from the rabbit and its reactions with various types of tissue culture cells. Virology 35:323
    [Google Scholar]
  28. Sjögren H. O., Jonsson N. 1963; Resistance against isotransplantation of mouse tumors induced by Rous sarcoma virus. Experimental Cell Research 32:618
    [Google Scholar]
  29. Stenback W., Van Hoosier G.JUN Trentin J. 1966; Virus particles in hamster tumors as revealed by electron microscopy. Proceedings of the Society for Experimental Biology and Medicine 122:1219
    [Google Scholar]
  30. Svec F., Altaner C., Hlavay E. 1966; Pathogenicity for rats of a strain of chicken sarcoma virus. Journal of the National Cancer Institute 36:389
    [Google Scholar]
  31. Svoboda J. 1960; Presence of chicken tumour virus in the sarcoma of the adult rat inoculated after birth with Rous sarcoma tissue. Nature; London: 186980
    [Google Scholar]
  32. Svoboda J. 1964; Malignant interaction of Rous virus with mammalian cells in vivo and in vitro. National Cancer Institute Monograph 17:277
    [Google Scholar]
  33. Svoboda J. 1966; Basic aspects of the interaction of oncogenic viruses with heterologous cells. International Review of Experimental Pathology 5:25
    [Google Scholar]
  34. Svoboda J., Klement V. 1963; Formation of delayed tumours in hamsters inoculated with Rous virus after birth and finding of infectious Rous virus in induced tumour P1. Folia Biologica 9:403
    [Google Scholar]
  35. De-Thé G., O’Connor T. 1966; Structure of a murine leukemia virus after disruption with tween-ether and comparison with two myxoviruses. Virology 28:713
    [Google Scholar]
  36. Thomas J. A., Delain E., Hollande E. 1967; Morphogenèse d’un virus du hamster associé A la souche BHK ou à des tumeurs. Compte rendu hebdomadaire des séances de l’Académie des sciences 264:785
    [Google Scholar]
  37. Valentine A. F., Bader J. P. 1968; Production of virus by mammalian cells transformed by Rous sarcoma and murine sarcoma viruses. Journal of Virology 2:224
    [Google Scholar]
  38. Venable J. H., Coggeshall R. 1965; A simplified lead citrate stain for use in electron microscopy. Journal of Cell Biology 25:407
    [Google Scholar]
  39. Veselý P., Svoboda J., Donner L. 1966; Line of hamster cells transformed BY the Schmidt-Ruppin strain of Rous virus in vitro. Folia Biologica 12:81
    [Google Scholar]
  40. Vogt P. 1965; Avian tumor viruses. Advances in Virus Research 11:293
    [Google Scholar]
  41. Vogt P. K. 1967; A virus released by ‘nonproducing’ Rous sarcoma cells. Proceedings of the National Academy of Sciences of the United States of America 58:801
    [Google Scholar]
  42. Weiss R. 1967; Spontaneous virus production from ‘non-virus-producing’ Rous sarcoma cells. Virology 32:719
    [Google Scholar]
  43. Weiss R. 1969; Biological properties of Bryan strain Rous sarcoma virus synthesized in the absence of helper virus. Journal of General Virology 5:511
    [Google Scholar]
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