1887

Abstract

Summary

We have isolated two plaque size variants of Theiler’s murine encephalomyelitis virus (TMEV) strain DA. One variant, TMEV-C (C), produced large plaques, while the other, TMEV-D (D), produced small plaques in L-2 cells. The D variant yielded a lower titre in BHK cells and had a significantly slower growth rate when compared to C and DA. In contrast, D replicated to a higher titre in the central nervous system (CNS) of infected mice than the large plaque counterpart and DA. Furthermore, the D (but not C) variant was temperature-sensitive, replicating 130- to 500-fold more at 37 °C than at 39 °C. Although D, C and DA were able to establish persistent CNS infections in mice, only the D variant and DA induced demyelinating disease in SJL/J mice. Therefore persistence of TMEV in the CNS is not sufficient to produce demyelinating disease. These two variants of the DA strain of TMEV will be useful for study of the viral genetic elements important in the mechanism of virus-induced demyelination.

Keyword(s): demyelination , plaque variants and TMEV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-9-2413
1988-09-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/9/JV0690092413.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-9-2413&mimeType=html&fmt=ahah

References

  1. BRAHIC M., STROOP W. G., BARINGER J. R. 1981; Theiler’s virus persists in glial cells during demyelinating disease. Cell 26:123–128
    [Google Scholar]
  2. COLTER J. S., CAMPBELL J. B., HATCH L. R. 1964; The pathogenicity to mice of three variants of mengoencephalomyelitis virus. Journal of Cellular and Comparative Physiology 65:229–236
    [Google Scholar]
  3. DAL CANTO M. C., LIPTON H. L. 1975; Primary demyelination in Theiler’s virus infection. An ultrastructural study. Laboratory Investigation 33:626–637
    [Google Scholar]
  4. DAL CANTO M. C., LIPTON H. L. 1982; Ultrastructural immunohistochemical localization of virus in acute and chronic demyelinating Theiler’s virus infection. American Journal of Pathology 106:20–29
    [Google Scholar]
  5. DANIELS I. B., PAPPENHEIMER A. M., RICHARDSON S. 1952; Observations on encephalomyelitis of mice (DA strain). Journal of Experimental Medicine 96:517–530
    [Google Scholar]
  6. LEHRICH J. R., ARNASON B. G. W., HOCHBERG F. H. 1976; Demyelinative myelopathy in mice induced by the DA virus. Journal of Neurological Science 29:149–160
    [Google Scholar]
  7. LIPTON H. L. 1975; Theiler’s virus infection in mice: an unusual biphasic disease process leading to demyelination. Infection and Immunity 11:1147–1155
    [Google Scholar]
  8. LIPTON H. L. 1980; Persistent Theiler’s murine encephalomyelitis virus infection in mice depends on plaque size. Journal of General Virology 46:169–177
    [Google Scholar]
  9. LIPTON H. L., MILLER S., MELVOLD R., FUJINAMI R. 1986 Theiler’s murine encephalomyelitis virus (TMEV) infection in mice as a model for multiple sclerosis. Concepts in Viral Pathogenesis 2248–254 Edited by Notkins A. L., Oldstone M. B. A. Wien & New York: Springer-Verlag;
    [Google Scholar]
  10. NITAYAPHAN S., TOTH M. M., ROOS R. 1985; Localization of a neutralization site of Theiler’s murine encephalomyelitis viruses. Journal of Virology 56:887–895
    [Google Scholar]
  11. RODRIGUEZ M., LEIBOWITZ J. L., LAMPERT P. W. 1983a; Persistent infection of oligodendrocytes in Theiler’s virus-induced encephalomyelitis. Annals of Neurology 13:426–433
    [Google Scholar]
  12. RODRIGUEZ M., LEIBOWITZ J. L., POWELL H. C., LAMPERT P. W. 1983b; Neonatal infection with the Daniels strain of Theiler’s murine encephalomyelitis virus. Laboratory Investigation 49:672–679
    [Google Scholar]
  13. RODRIGUEZ M., LAFUSE W. P., LEIBOWITZ J., DAVID C. S. 1986; Partial suppression of Theiler’s virus-induced demyelination in vivo by administration of monoclonal antibodies to immune response gene products (Ia) antigens. Neurology 36:964–970
    [Google Scholar]
  14. RODRIGUEZ M., OLESZAK E., LEIBOWITZ J. 1987; Theiler’s murine encephalomyelitis: a model of demyelination and persistence of virus. CRC Critical Reviews in Immunology 7:325–365
    [Google Scholar]
  15. TAKEMORI N., NOMURA S., MORIOKA M. M., KITAOKA M. 1957; Minute plaque mutant of type 2 poliovirus. Science 126:924–925
    [Google Scholar]
  16. TAKEMOTO K. K. 1966; Plaque mutants of animal viruses. Progress in Medical Virology 8:314–348
    [Google Scholar]
  17. TAKEMOTO K. K., HABEL K. 1959; Virus-cell relationship in a carrier culture of HeLa cells and Coxsackievirus Ag virus. Virology 7:28–44
    [Google Scholar]
  18. THEILER M. 1937; Spontaneous encephalomyelitis of mice - a new disease. Journal of Experimental Medicine 65:705–719
    [Google Scholar]
  19. VOGT M., DULBECCO R., WENNER H. A. 1957; Mutants of poliomyelitis viruses with reduced efficiency of plating in acid medium and reduced neuropathogenicity. Virology 4:141–155
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-9-2413
Loading
/content/journal/jgv/10.1099/0022-1317-69-9-2413
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error