1887

Abstract

Summary

Complementary DNA libraries were synthesized using the genomic RNAs of potato leafroll virus (PLRV) and beet western yellows virus (BWYV), by random as well as oligo(dT) priming of polyadenylated RNA, and cloned into pBR322. Two restriction endonuclease maps were constructed, extending to 6·1 kbp and 5·5 kbp for the PLRV and BWYV genomes respectively. The 3′ ends of genomic RNAs were verified by sequence analysis of oligo(dT)-primed clones; 5′-terminal sequences of PLRV and BWYV were not detected among the cDNA fragments mapped. Selected clones were used to demonstrate specific virus detection in total nucleic acid preparations of PLRV- or BWYV-infected Rydb. One PLRV-derived clone showed cross-hybridization with purified BWYV RNA.

Keyword(s): BWYV , luteoviruses and PLRV
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/content/journal/jgv/10.1099/0022-1317-69-9-2397
1988-09-01
2024-11-12
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References

  1. BARBARA D. J., KAWATA E. E., UENG P. P., LISTER R. M., LARKINS B. A. 1987; Production of cDNA clones from the MAV isolate of barley yellow dwarf virus. Journal of General Virology 68:2419–2427
    [Google Scholar]
  2. BERK A. J., SHARP P. A. 1977; Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12:721–732
    [Google Scholar]
  3. BERK A. J., SHARP P. A. 1978; Spliced early mRNAs of simian virus 40. Proceedings of the National Academy of Sciences, U.S.A 75:1274–1278
    [Google Scholar]
  4. BIRNBOIM H. C., DOLY J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  5. CARRINGTON J. C., MORRIS T. J. 1984; Complementary DNA cloning and analysis of carnation mottle virus RNA. Virology 139:22–31
    [Google Scholar]
  6. CASPER R. 1988 Luteoviruses. The Plant Viruses: Polyhedral Virions with Monopartite RNA Genomes 3235–258 Edited by Koenig R. New York: Plenum Press;
    [Google Scholar]
  7. CHANSILPA N. 1987 Serological and epidemiological investigations of potato leafroll virus and beet western yellows virus Ph.D. thesis, University of Göttingen
    [Google Scholar]
  8. FEINBERG A. P., VOGELSTEIN B. 1983; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  9. FEINBERG A. P., VOGELSTEIN B. 1984; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Addendum. Analytical Biochemistry 137:266–267
    [Google Scholar]
  10. GRUNSTEIN M., HOGNESS D. S. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences, U.S.A 72:3961–3965
    [Google Scholar]
  11. GUBLER V., HOFFMAN B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  12. HANAHAN D. 1983; Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:557–580
    [Google Scholar]
  13. HENIKOFF S. 1984; Unidirectional digestion with exonuclease III creates target breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  14. HEWINGS A. D., D’ARCY C. J. 1983; Characterization of two luteoviruses. Phytopathology 73:789–790
    [Google Scholar]
  15. JAYASENA K. W., RANDLES I. W., BARNETT O. W. 1984; Synthesis of a complementary DNA probe specific for detecting subterranean clover red leaf virus in plants and aphids. Journal of General Virology 65:109–117
    [Google Scholar]
  16. MCMASTER G. K., CARMICHAEL G. G. 1977; Analysis of single- and double-stranded nucleic acids on Polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U.S.A 74:4835–4838
    [Google Scholar]
  17. MAISS E., BREYEL E., CASPER R., LESEMANN D. E. 1987; Detection of plum pox virus by isolation of double-stranded ribonucleic acid (dsRNA). Bulletin Organization Européenne et Méditerranéene pour la Protection des Plantes/European and Mediterranean Plant Protection Organization Bulletin 17:91–95
    [Google Scholar]
  18. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor laboratory;
    [Google Scholar]
  19. MATTHEWS R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  20. MAULE A. J., HULL R., DONSON J. 1983; The application of spot hybridization to the detection of DNA and RNA viruses in plant tissues. Journal of Viroiogical Methods 6:215–224
    [Google Scholar]
  21. MAYO M. A., BARKER H., ROBINSON D. J., TAMADA T., HARRISON B. D. 1982; Evidence that potato leafroll virus RNA is positive-stranded, is linked to a small protein and does not contain polyadenylate. Journal of General Virology 59:163–167
    [Google Scholar]
  22. MORRIS T. J., DODDS J. A. 1979; Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 65:854–858
    [Google Scholar]
  23. RIGBY P. W. J., DIECKMANN M., RHODES C., BERG P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  24. ROCHOW W. F., DUFFUS I. E. 1981 Luteovirus and yellow diseases. Handbook of Plant Virus Infections and Comparative Diagnosis147–170 Edited by Kurstak E. Amsterdam, New York & Oxford: Elsevier/North-Holland;
    [Google Scholar]
  25. ROWHANI A., STACE-SMITH R. 1979; Purification and characterization of potato leafroll virus. Virology 98:45–54
    [Google Scholar]
  26. SANGER F., NICKLEN S., COULSON A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  27. SCHLEICHER & SCHUELL 1981 Methods for the Transfer of DNA, RNA and Protein to Nitrocellulose and Diazotized Paper Solid Supports Keene: Schleicher & Schuell;
    [Google Scholar]
  28. SOUTHERN E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  29. TAKANAMI Y., KUBO S. 1979; Enzyme-assisted purification of two phloem-limited plant viruses: tobacco necrotic dwarf and potato leafroll. Journal of General Virology 44:153–159
    [Google Scholar]
  30. WATERHOUSE P. M., GERLACH W. L., MILLER W. A. 1986; Serotype-specific and general luteovirus probes from cloned cDNA sequences of barley yellow dwarf virus. Journal of General Virology 67:1273–1281
    [Google Scholar]
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