1887

Abstract

Summary

Twelve Palyam serogroup orbiviruses isolated in Zimbabwe from aborted cattle foetuses, plus one isolated from the visceral organs of a cow and another from vulture faeces, were examined in comparison with known members of the serogroup by complement fixation, indirect immunofluorescence, fluorescent focus reduction neutralization tests and PAGE of the segmented, dsRNA genomes. The viruses were indistinguishable from known members of the serogroup by complement fixation and indirect immunofluorescence, but two novel viruses, for which the names Gweru and Marondera are proposed, and two previously described viruses, Nyabira and Abadina, were identified by neutralization tests. The dsRNA profiles of Abadina serotype isolates differed from that of the Abadina prototype virus, indicating that different electropherotypes may occur within serotypes.

Keyword(s): orbivirus , Palyam serogroup and serology
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1988-09-01
2022-01-21
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References

  1. ANONYMOUS 1973-1982 Annual Reports of the Veterinary Research Laboratory, Harare, Zimbabwe1973–1982
    [Google Scholar]
  2. BLACKBURN N. K., SWANEPOEL R. 1980; An investigation of flavivirus infections of cattle in Zimbabwe, Rhodesia with particular reference to Wesselsbron virus. Journal of Hygiene 85:1–33
    [Google Scholar]
  3. BLACKBURN N. K., SEARLE L., PHELPS R. J. 1985; Viruses isolated from Culicoides (Diptera: Ceratopogonidae) caught at the veterinary research farm, Mazowe, Zimbabwe. Journal of the Entomological Society of Southern Africa 48:331–336
    [Google Scholar]
  4. CLARKE D. H., CASALS J. 1958; Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  5. EAGLE H. 1959; Amino acid metabolism in mammalian cell cultures. Science 130:432–437
    [Google Scholar]
  6. EATON B. T., GOULD A. R. 1987; Isolation and characterization of Orbivirus genotypic variants. Virus Research 6:363–382
    [Google Scholar]
  7. GORMAN B. M. 1983; On the evolution of orbiviruses. Intervirology 20:169–180
    [Google Scholar]
  8. HUBBERT W. T., BOOTH G. D., BOLTON W. D., DUNNE H. W., MCENTEE K., SMITH R. E., TOURTELLOTTE M. E. 1973; Bovine abortions in five north-eastern states, 1960-1970: evaluation of diagnostic laboratory data. Cornell Veterinarian 63:291–316
    [Google Scholar]
  9. KARABATSOS N. 1985 International Catalogue of Arboviruses San Antonio: American Society of Tropical Medicine and Hygiene;
    [Google Scholar]
  10. KIRKBRIDE C A., BICKNELL E. J., REED D. E., ROBL M. G., KNUDSON W. U., WOHLGEMUTH K. 1973; A diagnostic survey of bovine abortion and stillbirth in the northern plains states. Journal of the American Veterinary Medical Association 162:556–560
    [Google Scholar]
  11. KNUDSON D. L., BUTTERFIELD W. K., SHOPE R. E., WALTON T. E., CAMPBELL C H. 1982; Electrophoretic comparison of the genomes of North American bluetongue viruses, one Australian bluetongue virus, and three other related orbiviruses. Veterinary Microbiology 7:285–293
    [Google Scholar]
  12. KNUDSON D. L., TESH R. B., MAIN A. J., ST GEORGE T. D., DIGOUTTE J. P. 1984; Characterization of the Palyam serogroup viruses (Reoviridae: Orbivirus). Intervirology 22:41–49
    [Google Scholar]
  13. LAEMMH U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. MUNDY P. J., FOGGIN C. M. 1981; Epileptiform seizures in captive African vultures. Journal of Wildlife Diseases 17:259–265
    [Google Scholar]
  15. OBERST R. D., SQUIRE K. R. E., STOTT J. L., CHUANG R. Y., OSBURN B. I. 1985; The coexistence of multiple bluetongue virus electropherotypes in individual cattle during natural infection. Journal of General Virology 66:1901–1909
    [Google Scholar]
  16. PEDLEY S., HUNDLEY F., CHRYSTIE I., MCCRAE M. A., DESSELBERGER U. 1984; The genomes of rotaviruses isolated from chronically infected immuhodeficient children. Journal of General Virology 65:1141–1150
    [Google Scholar]
  17. SAMAL S. K., EL-HUSSEIN A., HOLBROOK F. R., BEATY B. J., RAMIG R. F. 1987a; Mixed infection of Culkoides variipennis with bluetongue virus serotypes 10 and 17: evidence for high frequency assortment in the vector. Journal of General Virology 68:2319–2329
    [Google Scholar]
  18. SAMAL S. K., LIVINGSTON C. W., MCCONNELL S., RAMIG R. F. 1987b; Analysis of mixed infection of sheep with bluetongue virus serotypes 10 and 17: evidence for genetic reassortment in the vertebrate host. Journal of Virology 61:1086–1091
    [Google Scholar]
  19. SARTORELLI A. C, FISCHER D. S., DOWNS W. G. 1966; Use of sarcoma 180/TG to prepare hyperimmune ascitic fluid in the mouse. Journal of Immunology 96:676–682
    [Google Scholar]
  20. SQUIRE K. R. E., OSBURN B. I., CHUANG R. Y., DOI R. H. 1983; A survey of electropherotype relationships of bluetongue virus isolates from the western United States. Journal of General Virology 64:2103–2115
    [Google Scholar]
  21. STOTT I. L., OBERST R. D., CHANNELL M. B., OSBURN B. I. 1987; Genome segment reassortment between two serotypes of bluetongue virus in a natural host. Journal of Virology 61:2670–2674
    [Google Scholar]
  22. SWANEPOEL R., BLACKBURN N. K. 1976; A new member of the Palyam serogroup of orbiviruses. Veterinary Record 99:360
    [Google Scholar]
  23. SWANEPOEL R., BLACKBURN N. K., LANDER K. P., VICKERS D. B., LEWIS A. R. 1975; An investigation of infectious infertility and abortion of cattle. Rhodesian Veterinary Journal 6:42–55
    [Google Scholar]
  24. TRAVASSOS DA ROSA A. P. A., TESH R. B., PINHEIRO F. P., TRAVASSOS DA ROSA J. F. S., PERALTA P. H., KNUDSON D. L. 1984; Characterization of the Changuinola serogroup viruses (Reoviridae: Orbivirus). Interviroloey 21:38–49
    [Google Scholar]
  25. YASAMURA U., KAWAKITA Y. 1963; Studies on SV40 in relationship with tissue culture. Nippon Rinsho 21:1201–1209
    [Google Scholar]
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