1887

Abstract

Summary

Viral mutants that appeared during long-term persistent infections of mosquito cell cultures ( and ) with St Louis encephalitis virus were characterized. Evidence was obtained for the presence of temperature-sensitive mutants in the and persistently infected cultures, and small plaque mutants were predominant in all cultures except one of two cell cultures of . Virus from persistently infected cell cultures was growth-restricted in Vero and cells. One of two persistently infected cell cultures also produced viral mutants that were growth-restricted in cells. Further, Western blots of persistently infected cell extracts showed an overproduction of capsid (C) and envelope (E) structural proteins and reduced production of an 27K protein (p27) which was immunologically related to the E protein. In contrast, the production of E and C proteins in persistently infected cultures was consistent with the amount of infectious virus present, whereas p27 was relatively overproduced. These observations suggest that the host cell has an important influence on both the types and relative quantities of viral mutants that accumulate during long-term persistent infections.

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/content/journal/jgv/10.1099/0022-1317-69-9-2199
1988-09-01
2021-10-28
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References

  1. BRINTON M. A. 1982; Characterization of West Nile virus persistent infections in genetically resistant and susceptible mouse cells. I. Generation of defective nonplaquing virus particles. Virology 116:84–98
    [Google Scholar]
  2. BRINTON M. A. 1983; Analysis of extracellular West Nile virus particles produced by cell cultures from genetically resistant and susceptible mice indicates enhanced amplification of defective interfering particles by resistant cultures. Journal of Virology 46:860–870
    [Google Scholar]
  3. BRINTON M. A. 1987 Replication of flaviviruses. The Togaviridae and Flaviviridae327–373 Edited by Schlesinger S., Schlesinger M. J. New York & London: Plenum Press;
    [Google Scholar]
  4. BRINTON M. A., DAVIS J., SCHAEFER D. 1985; Characterization of West Nile virus persistent infections in genetically resistant and susceptible mouse cells. II. Generation of temperature-sensitive mutants. Virology 140:152–158
    [Google Scholar]
  5. BROWN D. T., CONDREAY L. D. 1986 Replication of alphaviruses in mosquito cells. The Togaviridae and Flaviviridae171–207 Edited by Schlesinger S., Schlesinger M. J. New York & London: Plenum Press;
    [Google Scholar]
  6. DURBIN R. K., STOLLAR V. 1984; A mutant of Sindbis virus with a host-dependent defect in maturation associated with hyperglycosylation of E2. Virology 135:331–344
    [Google Scholar]
  7. EATON B. T. 1981; Viral interference and persistence in Sindbis virus infected Aedes albopictus cells. Canadian Journal of Microbiology 27:563–567
    [Google Scholar]
  8. ELLIOTT R. M., WILKIE M. L. 1986; Persistent infection of Aedes albopictus C6/36 cells by Bunyamwera virus. Virology 150:21–32
    [Google Scholar]
  9. GOULD E. A., BUCKLEY A., CAMMACK N., BARRETT A. D. T., CLEGG J. C. S., ISHAK R., VARMA M. G. R. 1985; Examination of the immunological relationships between flaviviruses using yellow fever virus monoclonal antibodies. Journal of General Virology 66:1369–1382
    [Google Scholar]
  10. GROVE N. E. 1985 Characterization of monoclonal antibodies to Saint Louis encephalitis virus M.A. thesis, University of California, Berkeley
    [Google Scholar]
  11. HOLLAND J., SPINDLER K., HORODYSKI F., GRABAU E., NICHOL S., VANDEPOL S. 1982; Rapid evolution of RNA genomes. Science 215:1577–1585
    [Google Scholar]
  12. IGARASHI A. 1979; Characteristics of Aedes albopictus cells persistently infected with dengue viruses. Nature, London 280:690–691
    [Google Scholar]
  13. IGARASHI A., KOO R., STOLLAR V. 1977; Evolution and properties of Aedes albopictus cell cultures persistently infected with Sindbis virus. Virology 82:69–83
    [Google Scholar]
  14. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. MCSHARRY J., BENZINGER R. 1970; Concentration and purification of vesicular stomatitis virus by polyethylene glycol “precipitation”. Virology 40:745–746
    [Google Scholar]
  16. MAEDA S., HASHIMOTO K., SIMIZU B. 1979; Complementation between temperature-sensitive mutants isolated from Aedes albopictus cells persistently infected with western equine encephalitis virus. Virology 92:532–541
    [Google Scholar]
  17. NEWTON S. E., SHORT N. J., DALGARNO L. 1981; Bunyamwera virus replication in cultured Aedes albopictus (mosquito) cells: establishment of a persistent viral infection. Journal of Virology 38:1015–1024
    [Google Scholar]
  18. NG M. L., WESTAWAY E. G. 1980 Establishment of persistent infections by flaviviruses in Aedes albopictus cells. Invertebrate Systems In Vitro389–402 Edited by Kurstak E., Maramorosch K., Dubendorfer A. Amsterdam: Elsevier;
    [Google Scholar]
  19. RANDOLPH V. B., HARDY J. L. 1988; Establishment and characterization of St Louis encephalitis virus persistent infections in Aedes and Culex mosquito cell lines. Journal of General Virology 69:2189–2198
    [Google Scholar]
  20. RIEDEL B., BROWN D. T. 1977; Role of extracellular virus in the maintenance of the persistent infection induced in Aedes albopictus (mosquito) cells by Sindbis virus. Journal of Virology 23:554–561
    [Google Scholar]
  21. SARTORELLI A. C, FISCHER D. S., DOWNS W. G. 1966; Use of sarcoma 180/TG to prepare hyperimmune ascitic fluid in the mouse. Journal of Immunology 96:676–682
    [Google Scholar]
  22. SCHMALJOHN C., BLAIR C. D. 1977; Persistent infection of cultured mammalian cells by Japanese encephalitis virus. Journal of Virology 24:580–589
    [Google Scholar]
  23. SIMIZU B., MAEDA S. 1981; Growth patterns of temperature-sensitive mutants of Western equine encephalitis virus in cultured Aedes albopictus (mosquito) cells. Journal of General Virology 56:349–361
    [Google Scholar]
  24. STALDER J., REIGEL F., KOBLET H. 1983; Defective viral RNAs in Aedes albopictus C6/36 cells persistently infected with Semliki Forest virus. Virology 129:247–254
    [Google Scholar]
  25. STEACIE A. D., EATON B. T. 1984; Properties of defective interfering particles of Sindbis virus generated in vertebrate and mosquito cells. Journal of General Virology 65:333–341
    [Google Scholar]
  26. STOLLAR V. 1980 Togaviruses in cultured arthropod cells. The Togaviruses: Biology, Structure, Replication583–621 Edited by Schlesinger R. W. New York & London: Academic Press;
    [Google Scholar]
  27. TOOKER P., KENNEDY S. I. T. 1981; Semliki Forest virus multiplication in clones of Aedes albopictus cells. Journal of Virology 37:589–600
    [Google Scholar]
  28. WESTAWAY E. G., MCKLMM J. L., MCLEOD L. G. 1977; Heterogeneity among flavivirus proteins separated in slab gels. Archives of Virology 53:305–312
    [Google Scholar]
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