Viral mutants that appeared during long-term persistent infections of mosquito cell cultures ( and ) with St Louis encephalitis virus were characterized. Evidence was obtained for the presence of temperature-sensitive mutants in the and persistently infected cultures, and small plaque mutants were predominant in all cultures except one of two cell cultures of . Virus from persistently infected cell cultures was growth-restricted in Vero and cells. One of two persistently infected cell cultures also produced viral mutants that were growth-restricted in cells. Further, Western blots of persistently infected cell extracts showed an overproduction of capsid (C) and envelope (E) structural proteins and reduced production of an 27K protein (p27) which was immunologically related to the E protein. In contrast, the production of E and C proteins in persistently infected cultures was consistent with the amount of infectious virus present, whereas p27 was relatively overproduced. These observations suggest that the host cell has an important influence on both the types and relative quantities of viral mutants that accumulate during long-term persistent infections.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error