1887

Abstract

Summary

Persistent infections with St Louis encephalitis (SLE) virus were established in three mosquito cell lines ( and ) and were maintained for over 2 years. All three persistently infected cell cultures shared two features: (i) no overt cytopathic effect and (ii) a relatively high proportion of cells infected (41 to 85%). The persistently infected cultures were resistant to superinfection with the homologous virus but not heterologous viruses. Two significant differences were observed between the and persistently infected cell cultures: (i) viral titres in the and cell cultures decreased slowly over time (the decrease was particularly marked in the cell cultures), whereas titres in the cell cultures remained relatively constant and (ii) the addition of anti-SLE virus antibody led to decreased virus production in the cell cultures (one of two cultures was cured of infection), whereas antibody had no effect on the persistently infected cell cultures. These results suggest that there may be significant differences in the regulation of viral replication and the maintenance of flavivirus persistent infections in mosquito cell lines of different origins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-9-2189
1988-09-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/9/JV0690092189.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-9-2189&mimeType=html&fmt=ahah

References

  1. BRINTON M. A. 1982; Characterization of West Nile virus persistent infections in genetically resistant and susceptible mouse cells. I. Generation of defective nonplaquing virus particles. Virology 116:84–98
    [Google Scholar]
  2. BROWN D. T., CONDREAY L. D. 1986 Replication of alphaviruses in mosquito cells. The Togaviridae and Flaviviridae171–207 Edited by Schlesinger S., Schlesinger M. J. New York & London: Plenum Press;
    [Google Scholar]
  3. CAHOON B. E., HARDY J. L., REEVES W. C. 1978; Initiation and characterization of a diploid cell line from larval tissues of Aedes dorsalis (Meigen). In Vitro 14:255–260
    [Google Scholar]
  4. CAHOON B. E., HARDY J. L., REEVES W. C. 1979; Growth of California encephalitis and other viruses in Aedes dorsalis (Diptera: Culicoidae) cell cultures. Journal of Medical Entomology 16:104–111
    [Google Scholar]
  5. CHAO J., BALL G. H. 1976 A comparison of amino acid utilization by cell lines of Culex tarsalis and Culex pipiens. Invertebrate Tissue Culture: Applications in Medicine, Biology, and Agriculture263–266 Edited by Kurstak E., Maramorosch K. New York & London: Academic Press;
    [Google Scholar]
  6. HSU S. M., RAINE L., FANGER H. 1981; The use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques. A comparison between ABC and unlabeled antibody PAP procedures. Journal of Histochemistry and Cytochemistry 29:577–580
    [Google Scholar]
  7. IGARASHI A. 1978; Isolation of a Singh’s Aedes albopictus cell clone sensitive to dengue and Chikungunya viruses. Journal of General Virology 40:531–544
    [Google Scholar]
  8. IGARASHI A. 1979; Characteristics of Aedes albopictus cells persistently infected with dengue viruses. Nature, London 280:690–691
    [Google Scholar]
  9. IGARASHI A., KOO R., STOLLAR V. 1977; Evolution and properties of Aedes albopictus cell cultures persistently infected with Sindbis virus. Virology 82:69–83
    [Google Scholar]
  10. KATZ E., GOLDBLUM N. 1968; Establishment, steady state and cure of a chronic infection of LLC cells with West Nile virus. Archiv für die gesamte Virusforschung 25:69–82
    [Google Scholar]
  11. KUNO G. 1982; Persistent infection of a nonvector mosquito cell line (TRA-171) with dengue virus. Intervirology 18:45–55
    [Google Scholar]
  12. MONATH T. P. 1980 Epidemiology. St Louis Encephalitis239–312 Edited by Monath T. P. Washington, D.C.: American Public Health Association;
    [Google Scholar]
  13. NG M. L., WESTAWAY E. G. 1980 Establishment of persistent infections by flaviviruses in Aedes albopictus cells. Invertebrate Systems In Vitro389–402 Edited by Kurstak E., Maramorosch K., Dubendorfer A. Amsterdam: Elsevier;
    [Google Scholar]
  14. RANDOLPH V. B., HARDY J. L. 1988; Phenotypes of St Louis encephalitis virus mutants produced in persistently infected mosquito cell cultures. Journal of General Virology 69:2199–2207
    [Google Scholar]
  15. RIEDEL B., BROWN D. T. 1977; Role of extracellular virus in the maintenance of the persistent infection induced in Aedes albopictus (mosquito) cells by Sindbis virus. Journal of Virology 23:554–561
    [Google Scholar]
  16. SARTORELLI A. C, FISCHER D. S., DOWNS W. G. 1966; Use of sarcoma 180/TG to prepare hyperimmune ascitic fluid in the mouse. Journal of Immunology 96:676–682
    [Google Scholar]
  17. SCHMALJOHN C., BLAIR C. D. 1977; Persistent infection of cultured mammalian cells by Japanese encephalitis virus. Journal of Virology 24:580–589
    [Google Scholar]
  18. SINGH K. R. P. 1967; Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Current Science 36:506–508
    [Google Scholar]
  19. STOLLAR V. 1980 Togaviruses in cultured arthropod cells. The Togaviruses: Biology, Structure, Replication583–621 Edited by Schlesinger R. W. New York & London: Academic Press;
    [Google Scholar]
  20. VOLKMAN L. E., GOLDSMITH P. A. 1982; Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro. Applied and Environmental Microbiology 44:227–233
    [Google Scholar]
  21. WESTAWAY E. G., BRINTON M. A., GAIDAMOVICH S. YA., HORZINEK M. C, IGARASHI A., KÄÄRIÄINEN L., LVOV D. K., PORTERFIELD J. S., RUSSELL P. K., TRENT D. W. 1985; Togaviridae. Intervirology 24:125–139
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-9-2189
Loading
/content/journal/jgv/10.1099/0022-1317-69-9-2189
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error