Persistent infections with St Louis encephalitis (SLE) virus were established in three mosquito cell lines ( and ) and were maintained for over 2 years. All three persistently infected cell cultures shared two features: (i) no overt cytopathic effect and (ii) a relatively high proportion of cells infected (41 to 85%). The persistently infected cultures were resistant to superinfection with the homologous virus but not heterologous viruses. Two significant differences were observed between the and persistently infected cell cultures: (i) viral titres in the and cell cultures decreased slowly over time (the decrease was particularly marked in the cell cultures), whereas titres in the cell cultures remained relatively constant and (ii) the addition of anti-SLE virus antibody led to decreased virus production in the cell cultures (one of two cultures was cured of infection), whereas antibody had no effect on the persistently infected cell cultures. These results suggest that there may be significant differences in the regulation of viral replication and the maintenance of flavivirus persistent infections in mosquito cell lines of different origins.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error