1887

Abstract

Summary

Cytotoxic T lymphocytes (CTLs) were generated when spleen cells from mice infected with viruses causing haemorrhagic fever with renal syndrome were stimulated with syngeneic cells infected with viruses. These cytotoxic effector cells, with Lyt2 L3T4 markers on their surface, demonstrated H-2 restriction. CTLs induced by Hantaan virus (76–118 strain) or Seoul virus (B-1 strain) showed cross-reactivity with infected target cells. Hantaan virus infection induced a higher CTLs response than Seoul virus infection, although the antibody responses to these two viruses and the replication of the two virus strains in athymic nude mice were not significantly different. Viral antigen detected with a monoclonal antibody reacting with nucleocapsid antigen was observed mainly in the cytoplasm of macrophages infected with Hantaan virus, but in the nucleus of cells infected with Seoul virus. The major viral antigens recognized by CTLs are discussed on the basis of these findings.

Keyword(s): CTL , hantavirus , HV and Seoul virus
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-9-2179
1988-09-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/9/JV0690092179.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-9-2179&mimeType=html&fmt=ahah

References

  1. ASADA H., TAMURA M., KONDO K., OKUNO Y., TAKAHASHI Y., DOHI Y., NAGAI T., KURATA T., YAMANISHI K. 1987; Role of T lymphocyte subsets in protection and recovery from Hantaan virus infection in mice. Journal of General Virology 68:1961–1969
    [Google Scholar]
  2. DANTAS J. R. JR, OKUNO Y., ASADA H., TAMURA M., TAKAHASHI M., TANISHITA O., TAKAHASHI Y., KURATA T., YAMANISHI K. 1986; Characterization of glycoproteins of viruses causing hemorrhagic fever with renal syndrome (HFRS) using monoclonal antibodies. Virology 151:379–384
    [Google Scholar]
  3. DANTAS J. R. JR, OKUNO Y., TANISHITA O., TAKAHASHI Y., TAKAHASHI M., KURATA T., LEE H. W., YAMANISHI K. 1987; Viruses of hemorrhagic fever with renal syndrome (HFRS) grouped by immunoprecipitation and hemagglutination inhibition. Intervirology 27:161–165
    [Google Scholar]
  4. GAJDUSEK D. C. 1962; Virus hemorrhagic fever: special reference to hemorrhagic fever with renal syndrome (epidemic hemorrhagic fever). Journal of Pediatrics 60:841–857
    [Google Scholar]
  5. HUNG T., XIA S.-M., ZHAO T. X., ZHOU J. Y., SONG G., LIAO G. X. H., YE W. W., CHU Y. L., HANG C. S. 1983; Morphological evidence for identifying the viruses of hemorrhagic fever with renal syndrome as candidate members of the Bunyaviridae family. Archives of Virology 78:137–144
    [Google Scholar]
  6. HUNG T., CHOU Z., ZHAO T., XIA S., HANG C. 1985; Morphology and morphogenesis of viruses of hemorrhagic fever with renal syndrome (HFRS). Intervirology 23:97–108
    [Google Scholar]
  7. KIM G. R., MCKEE K. T. JR 1985; Pathogenesis of Hantaan virus infection in suckling mice: clinical, virologie, and serologic observations. American Journal of Tropical Medicine and Hygiene 34:388–395
    [Google Scholar]
  8. KITAMURA T., MORITA C, KOMATSU T., SUGIYAMA K., ARIKAWA J., SHIGA S., TAKEDA H., AKAO Y., IMAIZUMI K., OYA A., HASHIMOTO N., URASAWA S. 1983; Isolation of virus causing hemorrhagic fever with renal syndrome (HFRS) through a cell culture system. Japan Journal of Medical Science and Biology 36:17–25
    [Google Scholar]
  9. KURATA T., TSAI T. F., BAUER S. P., MCCORMICK J. B. 1983; Immunofluorescence studies of disseminated Hantaan virus infection of suckling mice. Infection and Immunity 41:391–398
    [Google Scholar]
  10. LÄHDEVIRTA J. 1982; Clinical features of HFRS in Scandinavia as compared with east Asia. Scandinavian Journal of Infectious Diseases supplement 36:93–95
    [Google Scholar]
  11. LEE H. W. 1978 Korean hemorrhagic fever. Ebola Virus Hemorrhagic Fever331–343 Edited by Pattyn S. R. New York & Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  12. LEE H. W., LEE P. W., JOHNSON K. M. 1978; Isolation of the etiologic agent of Korean hemorrhagic fever. Journal of Infectious Diseases 137:298–308
    [Google Scholar]
  13. LEE H. W., FRENCH G. R., LEE P. W., BAEK L. J., TSUCHIYA K., FOULKE R. S. 1981a; Observation on natural and laboratory infection of rodents with the etiologic agent of Korean hemorrhagic fever. American Journal of Tropical Medicine and Hygiene 30:477–482
    [Google Scholar]
  14. LEE H. W., LEE P. W., BAEK L. J., SONG C. K., SEONG I. W. 1981b; Intraspecific transmission of Hantaan virus, etiologic agent of Korean hemorrhagic fever, in the rodent Apodemus agrarius. American Journal of Tropical Medicine and Hygiene 30:1106–1112
    [Google Scholar]
  15. LEE P. W., AMYX H. L., GIBBS C. J. JR, GAJDUSEK D. C., LEE H. W. 1981; Propagation of Korean hemorrhagic fever virus in laboratory rats. Infection and Immunity 31:334–338
    [Google Scholar]
  16. LEE P. W., AMYX H. L., GAJDUSEK D. C, YANAGIHARA R. T., GOLDGABER D., GIBBS C. J. 1982; New haemorrhagic fever with renal syndrome-related virus in indigenous wild rodents in the United States. Lancet ii:1405
    [Google Scholar]
  17. LEE P. W., GIBBS C. J., GAJDUSEK D. C., YANAGIHARA R. 1985; Serotypic classification of hantaviruses by indirect immunofluorescent antibody and plaque reduction neutralization tests. Journal of Clinical Microbiology 22:940–944
    [Google Scholar]
  18. MCCORMICK J. B., SASSO D. R., PALMER E. L., KILEY M. P. 1982; Morphological identification of the agent of Korean hemorrhagic fever (Hantaan virus) as a member of the Bunyaviridae. Lancet i:765–768
    [Google Scholar]
  19. NAGAI T., TANISHITA O., TAKAHASHI Y., YAMANOUCHI T., DOMAE K., KONDO K., DANTAS J. R. JR, TAKAHASHI M., YAMANISHI K. 1985; Isolation of haemorrhagic fever with renal syndrome virus from leukocytes of rats and virus replication in cultures of rat and human macrophages. Journal of General Virology 66:1271–1278
    [Google Scholar]
  20. NAKAMURA T., YANAGIHARA R., GIBBS C. J., AMYX H. L., GAJDUSEK D. C. 1985a; Differential susceptibility and resistance of immunocompetent and immunodeficient mice to fatal Hantaan virus infection. Archives of Virology 86:109–120
    [Google Scholar]
  21. NAKAMURA T., YANAGIHARA R., GIBBS C. J., GAJDUSEK D. C. 1985b; Immune spleen cell-mediated protection against fatal Hantaan virus infection in infant mice. Journal of Infectious Diseases 151:691–697
    [Google Scholar]
  22. SCHMALJOHN C. S., DALRYMPLE J. M. 1983; Analysis of Hantaan virus RNA: evidence for a new genus of Bunyaviridae. Virology 131:482–491
    [Google Scholar]
  23. SCHMALJOHN C. S., HASTY S. E., HARRISON S. A., DALRYMPLE J. M. 1983; Characterization of Hantaan virions, the prototype virus of hemorrhagic fever with renal syndrome. Journal of Infectious Diseases 148:1005–1012
    [Google Scholar]
  24. SCHMALJOHN C. S., HASTY S. E., DALRYMPLE J. M., LEDUC J. W., LEE H. W., VAHERI A., TSAI T. F., REGNERY H. L., GOLDGABER D., LEE P. W. 1985; Antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome. Science 227:1041–1044
    [Google Scholar]
  25. SONG G., HANG C, QUI C, NI D., LIAO H., GAO G., DU Y., XU J., WU Y., ZHAO J., KONG B., WANG Z., ZHANG Z., SHEN H., ZHOU N. 1983; Etiologic studies of epidemic hemorrhagic fever (hemorrhagic fever with renal syndrome). Journal of Infectious Diseases 147:654–659
    [Google Scholar]
  26. SUGIYAMA K., MORIKAWA S., MATSUURA Y., TKACHENKO E. A., MORITA C, KOMATSU T., AKAO Y., KITAMURA T. 1987; Four serotypes of haemorrhagic fever with renal syndrome viruses identified by polyclonal and monoclonal antibodies. Journal of General Virology 68:979–987
    [Google Scholar]
  27. SWAIN S. L. 1981; Significance of Lyt phenotypes: Lyt2 antibodies block activities of T cells that recognize class I major histocompatibility complex antigens regardless of their function. Proceedings of the National Academy of Sciences, U.S.A 78:7101–7105
    [Google Scholar]
  28. SWAIN S. L. 1983; T cell subsets and the recognition of MHC class. Immunological Reviews 74:129–142
    [Google Scholar]
  29. TANISHITA O., TAKAHASHI Y., OKUNO Y., YAMANISHI K., TAKAHASHI M. 1984; Evaluation of focus reduction neutralization test with peroxidase-antiperoxidase staining technique for hemorrhagic fever with renal syndrome virus. Journal of Clinical Microbiology 20:1213–1215
    [Google Scholar]
  30. TANISHITA O., TAKAHASHI Y., OKUNO Y., TAMURA M., ASADA H., DANTAS I. R. JR, YAMANOUCHI T., DOMAE K., KURATA T., YAMANISHI K. 1986; Persistent infection of rats with haemorrhagic fever with renal syndrome virus and their antibody responses. Journal of General Virology 67:2819–2824
    [Google Scholar]
  31. TOWNSEND A. R. M., SKEHEL J. J. 1984; The influenza A virus nucleoprotein gene controls the induction of both subtype specific and cross-reactive cytotoxic T cells. Journal of Experimental Medicine 160:552–563
    [Google Scholar]
  32. TOWNSEND A. R. M., MCMICHAEL A. I., CARTER N. P., HUDDLESTON J. A., BROWNLEE G. G. 1984; Cytotoxic T Cell recognition of the influenza nucleoprotein and hemagglutinin expressed in transfected mouse L cells. Cell 39:13–25
    [Google Scholar]
  33. TOWNSEND A. R. M., GOTCH F. M., DAVEY J. 1985; Cytotoxic T cells recognize fragments of the influenza nucleoprotein. Cell 42:457–467
    [Google Scholar]
  34. TSAI T. F., BAUER S., MCCORMICK J. B., KURATA T. 1982; Intracerebral inoculation of suckling mice with Hantaan virus. Lancet ii:503–504
    [Google Scholar]
  35. WHITE J. D., SHIREY F. G., FRENCH G. R., HUGGINS J. W., BRAND O. M., LEE H. W. 1982; Hantaan virus, aetiological agent of Korean haemorrhagic fever, has Bunyaviridae-like morphology. Lancet i:768–771
    [Google Scholar]
  36. YAMANISHI K., DANTAS J. R. JR, TAKAHASHI M., YAMANOUCHI T., DOMAE K., KAWAMATA J., KURATA T. 1983; Isolation of hemorrhagic fever with renal syndrome (HFRS) virus from a tumor specimen in a rat. Biken Journal 26:155–160
    [Google Scholar]
  37. YAMANISHI K., DANTAS J. R. JR, TAKAHASHI M., YAMANOUCHI T., DOMAE K., TAKAHASHI Y., TANISHITA O. 1984; Antigenic differences between two viruses, isolated in Japan and Korea, that cause hemorrhagic fever with renal syndrome. Journal of Virology 52:231–237
    [Google Scholar]
  38. YAMANOUCHI T., DOMAE K., TANISHITA O., TAKAHASHI Y., YAMANISHI K., TAKAHASHI M., KURATA T. 1984; Experimental infection in newborn mice and rats by hemorrhagic fever with renal syndrome (HFRS) virus. Microbiology and Immunology 28:1345–1353
    [Google Scholar]
  39. YANAGIHARA R., SVEDMYR A., AMYX H. L., LEE P. W., GOLDGABER D., GAJDUSEK D. C, GIBBS C. J., NYSTRÖM K. 1984; Isolation and propagation of nephropathia epidemica virus in bank voles. Scandinavian Journal of Infectious Diseases 16:225–228
    [Google Scholar]
  40. YANAGIHARA R., GOLDGABER D., GAJDUSEK D. C. 1985a; Propagation of nephropathia epidemica virus in Mongolian gerbils. Journal of Virology 53:973–975
    [Google Scholar]
  41. YANAGIHARA R., AMYX H. L., GAJDUSEK D. C. 1985b; Experimental infection with Puumala virus, the etiologic agent of nephropathia epidemica, in bank voles (Clethrionomys glareolus). Journal of Virology 55:34–38
    [Google Scholar]
  42. YEWDELL J. W., BENNINK J. R., SMITH G. L., MOSS B. 1985; Influenza A virus nucleoprotein is a major target antigen for class-reactive anti-influenza A virus cytotoxic T lymphocytes. Proceedings of the National Academy of Sciences, U.S.A 82:1785–1789
    [Google Scholar]
  43. YEWDELL J. W., BENNINK J. R., MACKETT M., LEFRANCOIS L., LYLES D. S., MOSS B. 1986; Recognition of cloned vesicular stomatitis virus internal and external gene products by cytotoxic T lymphocytes. Journal of Experimental Medicine 163:1529–1538
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-9-2179
Loading
/content/journal/jgv/10.1099/0022-1317-69-9-2179
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error