Respiratory Syncytial Virus: Heterogeneity of Subgroup B Strains Free

Abstract

Summary

In order to investigate further possible structural differences among the two subgroups of respiratory syncytial virus (RSV), we analysed the antigenic characteristics and size of structural proteins of 20 subgroup A and 43 subgroup B strains by their reactions with monoclonal antibodies (MAbs) directed against the proteins of RSV using immunofluorescence, ELISA and radioimmunoprecipitation assays. The latter test also enabled determination of the size of different structural components. The 37 MAbs employed were generated by immunization with both subgroup A and B strains. They represented specificities for distinct epitopes on five different structural proteins. The subgroup A strains proved to be relatively uniform. The fusion (F) protein, nucleoprotein (NP) and matrix (M) proteins of all strains tested had the same and all except one strain had a phosphoprotein (P protein) of the same . The F and P proteins were lower in in B strains compared to A strains, which confirmed previous findings. The of the large surface glycoprotein (G protein) of subgroup A strains varied slightly, probably on the basis of differing glycosylation. By contrast, the subgroup B strains exhibited substantial variation in the of the G and also the P proteins and in reactivity with MAbs directed against the G and F proteins. Three size classes of the P protein were identified in B strains: 33K to 34K, 32K to 33K, and 31K to 32K. Twenty-seven subgroup B strains failed to react with four anti-G MAbs representing a single epitope, G2; the remaining 16 strains reacted with these MAbs. We designated these two sets of variants of B strains B1, which lacked the epitope, and B2, which had the epitope. The B1 strains also varied in the size of the G and P proteins. In contrast, all B2 strains had large G proteins and all except two strains had relatively large P proteins (33K to 34K). All subgroup B1 and B2 strains exhibited the same sizes of NP, F and M proteins. We conclude that the subgroup B strains of RSV include two variants, B1 and B2, and that the major difference between them resides in the G and P proteins.

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1988-09-01
2024-03-29
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References

  1. ÅKERLIND B., NORRBY E. 1986; Occurrence of respiratory syncytial virus subtypes A and B strains in Sweden. Journal of Medical Virology 19:241–249
    [Google Scholar]
  2. ANDERSON L. J., HIERHOLZER J. C, TSOU C, HENDRY R. M., FERNIE B. F., STONE Y., MCINTOSH K. 1985; Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. Journal of Infectious Diseases 151:626–633
    [Google Scholar]
  3. BELSHE R. B., BERNSTEIN J. M., DANSBY K. N. 1984 Respiratory syncytial virus. Textbook of Human Virology361–384 Edited by Belshe R. B. Littleton: PSG Publishing Co;
    [Google Scholar]
  4. CHANOCK R. M., ROIZMAN B., MYERS R. 1957; Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent. I. Isolation, properties and characterization. American Journal of Hygiene 66:281–290
    [Google Scholar]
  5. COATES H. V., KENDRICK L., CHANOCK R. M. 1963; Antigenic differences between two strains of respiratory syncytial virus. Proceedings of the Society for Experimental Biology and Medicine 112:958–964
    [Google Scholar]
  6. DOGGETT J. E., TAYLOR-ROBINSON D. 1964; Serological studies with respiratory syncytial virus. Archiv für die gesamte Virusforschung 15:601–608
    [Google Scholar]
  7. GIMENEZ H. B., CASH P., MELVIN W. T. 1984; Monoclonal antibodies to human respiratory syncytial virus and their use in comparison of different virus isolates. Journal of General Virology 65:963–971
    [Google Scholar]
  8. GIMENEZ H. B., HARDMAN N., KEIR H. M., CASH P. 1986; Antigenic variation between human respiratory syncytial virus isolates. Journal of General Virology 67:863–870
    [Google Scholar]
  9. HENDERSON F. W., COLLIER A. M., CLYDE W. A. JR, DENNY F. W. 1979; Respiratory syncytial virus infections, reinfections and immunity. A prospective longitudinal study in young children. New England Journal of Medicine 300:530–534
    [Google Scholar]
  10. HENDRY R. M., TALIS A. L., GODFREY E., ANDERSON L. J., FERNIE B. F., MCINTOSH K. 1986; Concurrent circulation of antigenically distinct strains of respiratory syncytial virus during community outbreaks. Journal of Infectious Diseases 153:291–297
    [Google Scholar]
  11. JOHNSON P. R. JR, OLMSTED R. A., PRINCE G. A., MURPHY B. R., ALLING D. W., WALSH E. E., COLLINS P. L. 1987; Antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups A and B: evaluation of the contributions of F and G glycoproteins to immunity. Journal of Virology 61:3163–3166
    [Google Scholar]
  12. MORGAN L. A., ROUTLEDGE E. G., WILLCOCKS M. M., SAMSON A. C. R., SCOTT R., TOMS G. L. 1987; Strain variation of respiratory syncytial virus. Journal of General Virology 68:2781–2788
    [Google Scholar]
  13. MUFSON M. A., ÖRVELL C, RAFNAR B., NORRBY E. 1985; Two distinct subtypes of human respiratory syncytial virus. Journal of General Virology 66:2111–2124
    [Google Scholar]
  14. MUFSON M. A., BELSHE R. B., ÖRVELL C., NORRBY E. 1987; Subgroup characteristics of respiratory syncytial virus strains recovered from children with two consecutive infections. Journal of Clinical Microbiology 25:1535–1539
    [Google Scholar]
  15. MUFSON M. A., BELSHE R. B, ÖRVELL C., NORRBY E. 1988; Respiratory syncytial virus epidemics: variable dominance of subgroups A and B strains among children, 1981-1986. Journal of Infectious Diseases 157:143–148
    [Google Scholar]
  16. NORRBY E., CHEN S. N., TOGASHI T., SHESHBERADARAN H., JOHNSON K. P. 1982; Five measles virus antigens demonstrated by use of mouse hybridoma antibodies in productively infected tissue culture cells. Archives of Virology 71:1–11
    [Google Scholar]
  17. NORRBY E., MUFSON M.A., SHESHBERADARAN H. 1986; Structural differences between subtype A and B strains of respiratory syncytial virus. Journal of General Virology 67:2721–2729
    [Google Scholar]
  18. ÖRVELL C., NORRBY E. 1980; Immunological relationships between homologous structural polypeptides of measles and canine distemper virus. Journal of General Virology 50:231–245
    [Google Scholar]
  19. ÖRVELL C, NORRBY E., MUFSON M. A. 1987; Preparation and characterization of monoclonal antibodies directed against five structural components of human respiratory syncytial virus subgroup B. Journal of General Virology 68:3125–3135
    [Google Scholar]
  20. SHESHBERADARAN H., CHEN S. N., NORRBY E. 1983; Monoclonal antibodies against five structural components of measles virus. I. Characterization of antigenic determinants on nine strains of measles virus. Virology 129:341–353
    [Google Scholar]
  21. STORCH G. A., PARK C. S. 1987; Monoclonal antibodies demonstrate heterogeneity in the G glycoprotein of prototype strains and clinical isolates of respiratory syncytial virus. Journal of Medical Virology 22:345–356
    [Google Scholar]
  22. WULFF H., WENNER H. A. 1964; Respiratory syncytial virus: observations on antigenic heterogeneity. Proceedings of the Society for Experimental Biology and Medicine 115:240–243
    [Google Scholar]
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