Fluctuation of oligo-2′,5′-adenylate synthetase (2-5AS) activity was examined in K562 cells infected with vaccine strains of measles virus (strains AIK-C and CAM-70) and mumps virus (strains Torii and Miyahara). Persistent infection was easily established in the mumps virus-infected cells without significant cytolysis or cell killing. In contrast, most of the cells infected with measles virus were killed by extensive cytolysis within 3 to 4 days. The small number of cells that did survive became persistently infected. That these persistently infected cells carried a virus antigen was confirmed by fluorescein isothiocyanate-labelled anti-measles virus rabbit antiserum and anti-mumps virus rabbit antiserum. The cells produced infectious progeny virus as well as interferon (IFN). Little induction of 2-5AS activity by IFN was demonstrated during the early stages of infection by these viruses. Similar results were observed in some of the persistently infected cells but not, however, K-CMP cells (K562 cells persistently infected with CAM-70) or K-MMP cells (K562 cells persistently infected with Miyahara). Failure to induce 2-5AS activity was unchanged in cells cultured for more than 6 months. The decrease of 2-5AS activity observed in K-MTP cells (K562 cells persistently infected with Torii) was the result of suppression of transcription of 2-5AS mRNA. On the other hand, a normal level of mRNA was found in K-AKP cells (K562 cells persistently infected with AIK-C). Therefore, it is suggested that the decrease of 2-5AS activity in K-AKP cells may be due to a failure to translate 2-5AS mRNA.
BARRYD. W., SULLIVANJ. L., LUCASS. J., DUNLAPR. C., ALBRECHTP.1976; Acute and chronic infection of human lymphoblastoid cell lines with measles virus. Journal of Immunology 116:89–98
CARTERM. J., WILLCOCKSM. M., TER MEULENV.1983; Defective translation of measles virus matrix protein in a subacute sclerosing panencephalitis cell line. Nature, London 305:153–155
CRESPIM., CHIUM. N., SCHOUBB. D., LYONSS. F.1986; Effect of interferon on Vero cells persistently infected with SSPE virus and lytically infected with measles virus. Archives of Virology 90:87–96
FUJIIN., OGUMAK.1986; Induction of oligo-2′,5′-adenylate synthetase in human lymphoid cells treated with 5-azacytosine and 5-iododeoxyuridine. Journal of General Virology 67:2521–2526
FUJIIN., MINAGAWAT., NAKANAA., KATOF., οHNOS.1983; Spontaneous production of y-interferon in cultures of T lymphocytes obtained from patients with Behcet’s disease. Journal of Immunology 130:1683–1686
FUJIIN., OGUMAK., YAMASHITAT., FUJINAGAK., KAKINUMAM., YASHIKIT.1988; Decrease of oligo-2′,5′-adenylate synthetase activity in Balb3T3 cells persistently infected with Moloney murine leukemia virus. Virus Research in press
HALLW. W., CHOPPINP. W.1981; Measles virus protein in the brain tissue of patients with subacute sclerosing panencephalitis. New England Journal of Medicine 304:1152–1155
JACOBSONS., MCFARLANDH. F.1982; Measles virus persistence in human lymphocytes: a role for virus-induced interferon. Journal of General Virology 63:351–357
MENNAJ. H., COLLINSA. R., FLANAGANT. D.1975; Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BMG/MV cell line. Infection and Immunity 11:152–158
RAVEN., CRKVANJAKOVR., BOEDTKERH.1979; Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels. Nucleic Acids Research 6:3559–3565
SHIOJIRIS., FUKUNAGAR., ICHIIY., SOKAWAY.1986; Structure and expression of a cloned cDNA for human (2′-5′) oligoadenylate synthetase. Journal of Biochemistry 99:1455–1464
TRUANTA. L., HALLUMJ. V.1977; A persistent infection of baby hamster kidney-21 cells with mumps virus and the role of temperature-sensitive variants. Journal of Medical Virology 1:49–67